VOLUME iATIONAL INSTITUTES OF HEALTH v: OFFICE OF THE DIRECTOR Animal Research Facilities Uling Static Microisolators VENTILATION DESIGN HANDBOOK ON National Institutes of Health Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators VOLUME II Farhad Memarzadeh Principal Investigator Division of Engineering Services Office of Research Services Office of the Director National Institutes of Health Bethesda, Maryland September 1998 1 VOLUME II: TABLE OF CONTENTS 1. INTRODUCTION ii-i 1.1 Outline of CFD Baseline Model II - 1 1.2 Whole Room Configurations II - 8 2. SUMMARY OF RESULTS OF WHOLE ROOM RUNS ii 29 3. RANKINGS OF RESULTS OF WHOLE ROOM RUNS ii 37 3.1 Introduction II - 37 4. WHOLE ROOM SIMULATION DATABASE ii 55 4.1 Description of Simulation Data Entry 11-55 4.2 Simulation Data Entries 11-57 n ) 3 0 . 3 5 " Volume II - Section I - Introduction Page II - 1 1. INTRODUCTION This volume contains a database of the results of the computational fluid dynamics (CFD) simulations for the research done on the National Institutes of Health Ventilation Design Handbook on Animal Research Facilities using Static Microisolators. Section 1 contains an overview of the basic room configuration studied and the variations considered. It is impossible to present all the data generated by the CFD simulations. The analysis of all the results produces an enormous quantity of data that would be difficult to present and interpret in its entirety. This volume contains summaries of the data in several forms. Section 2 contains histograms of mean values for each room considered, as they apply to both the scientists’ breathing zone and the cages, for temperature, relative humidity, and CO2 and NH3 concentrations. Section 3 contains rankings of the rooms based on different variables, for example, the selection of the room configuration with the lowest NH3 concentrations in the cages. Section 4 contains a two page summary of each room simulation, including a description of the room, mean values and standard deviations for all parameters, and histograms showing the distribution of values in the cages. 1.1 Outline of CFD Baseline Model A typical animal research facility of average size, air change rate, rack layout, mouse population, pressurization, and so on, was modeled as the baseline model for the CFD simulations. The general features of the room are shown in figure 1.01 and are listed below. Volume 11-2 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Figure 1.01 Overall Layout of Animal Room Basecase Description in brief The general features of the basecase room model were: Room-. 6.10m x 3.60m x 4.22m (20’ 0” x 12’ 0” x 9’ 0”) Door in short wall Sink in comer Laminar flow change station 5 racks Cages: Microisolator (with filter top) mouse cage 5 mice per cage Rack: Static system 6 shelves per rack 7 cages per shelf (42 cages per rack) Volume II - Section I - Introduction Paee II - 3 Supply. 2 radial supplies each providing 270cfm (0. 13m^/s) for a total of 15 ACH Supply discharge temperature of 18.8 °C (66 °F), set such that the exhaust air temperature was 22.2 °C (72.0 °F) 61 percent relative humidity (to provide 50 percent RH at 22.2 °C (72.0 °F) Exhausts’. 2 ceiling level exhausts removing 220cfm (0.1 mVs) each Makeup Air. lOOcfm (0.047m /s) coming from around the door Overall Geometry In the majority of the cases considered, the animal room occupied a floor area of 6.10m (20’ 0”) X 3.66m (12’ 0”). In some cases that were considered, the width of the room was increased from 3.66m to 4.22m (14’ 0”). The ceiling height in all cases was 2.74m (9’ 0”). There was only one door in the room, mounted centrally on one of the short walls. In all the displacement ventilation systems considered in this project, air was introduced through ceiling mounted diffusers. All devices were mounted flush with the ceiling surface; there was no ductwork present within the upper room volume. The various diffuser types considered in this project were all modeled using a combination of several boundary conditions that were validated previously (see volume I, section 4.2.2). All the air exited through general exhausts. The number and locations of the exhausts were varied. In line with common practice, there was an imbalance between the amount of air supplied to the room and exhausted from the room. This leads to an overall pressurization of the room relative to the rooms or corridors surrounding the room. The relative level of pressurization was a parameter considered in this study. The makeup air to compensate for the supply/exhaust imbalance was allowed to enter or leave the room through 6.35e-3m (0.25”) gaps on three sides of the door. The rooms considered in this project all contained five animal cage racks. The rooms also contained one of two alternative design change stations. A fuller description of these items is given below. The only other item within the room was a sink of 0.61m (24”) x 0.61m (24”) x 0.81m (32”) that was situated in one of the comers to the room. In all cases, the room was considered under dark period conditions, i.e., the lights were off and produced no additional heat load to the room. Dark period conditions were chosen because early experimental work had indicated that heat, CO2, and NH3 generation were higher in the dark period compared with periods in which the lights were on. Rack Model The overall dimensions of the rack were 1.52m (60”) x 0.61m (24”) x 1.83m (72”) high, as shown in figure 1.02. There were six shelves in the rack. The spacing of the shelves was 0.32m (12.75”) from top surface to top surface, with the lowest shelf at a height of 0.21m (8.25”) above the floor. The shelves were modeled as thin rectangular blocks. Details, such as the connecting ties between the shelves and the rollers on which the racks move, were not modeled because their effect on the overall flow field and gas concentration distributions was considered insignificant. Volume II - 4 Ventilation Design Handbook on Animal Research Facilities Usin£ Static Microisolators Figure 1.02 CFD Model of Cage Rack Located on the shelves of the racks were representations of the animal cages, shown in figure 1.02. The dimensions of the cage were 0.27m (10.7”) x 0.16m (6.38”) x 0.21m (8.39”) high, which maintained the volume of the original cage that had sloped sides. The sides of the cage were modeled as thin plates, with the thickness and conductivity of the plates set to those of the physical cage polycarbonate. The water bottle and food normally found in a cage were modeled as a single block in order to reduce the computational overhead. The volume of the block was the same as that of the bottle and food combined. The bedding of the cage was included as a rectangular block of 0.27m (10.7”) x 0.16m (6.38”) x 1.27e-2m (0.5”). The mice were modeled as a block of 0.11m (4.25”) x 8.57e-2m (3.38”) x 0.22m (0.88”), the same representation that was used in the experimental cage wind tunnel tests (see volume I, section 4.1.1). This simulated the effect of ‘huddling’ by the mice. The surface temperature of the block was fixed at 30.0 °C (86.0 °F), which is agreed to be a typical mouse body surface temperature. Surrounding this block, a source of CO 2 was defined at 2.12e-7 kg/s (0.76g/hr), which was based on the generation rate indicated for the dark period in the early tests on the effect of the Volume II - Section I - Introduction Pa 2 e II - 5 photoperiod on the mice. The supply air was assumed to have a zero concentration of CO 2 . This source allowed the additional concentration of CO 2 in the air to be calculated in the simulation. It also allowed the concentration of NH 3 , among other things, to be calculated by scaling even though it has a different molecular weight from both air and CO 2 . This was possible as the magnitude of the source was very small and the resulting concentrations were so low as to have a negligible effect on the density of the mixture of air, CO 2 , and NH 3 . In effect, the CO 2 and NH 3 are intimately mixed with, and flow with, the air. Experimental data later showed the generation rate of CO 2 was actually higher than the source used in the CFD simulations at 0.90 g/hr/lOOg mouse body weight. This means the concentration of CO 2 in the room and cages was derived from the simulated concentration multiplied by a scaling factor (0.90/0.76). The concentrations of NH3 in both the cages and the room were also derived by scaling the concentration with a factor specified in the post-processing of the data. This factor was assumed to vary according to the number of days that passed since the bedding in the cage was changed, along with the average relative humidity in the cages. See volume I, section 4.2. 1.2 for the experimental determination of the factors. Background levels of CO 2 and NH 3 were assumed to be zero. This means that all values quoted in the CFD section of the report are relative to the background level. If an absolute value for CO 2 is required, an additional amount in the range of SOOppm to 700ppm should be added for most locations. The remaining cage boundary conditions are associated with the transfer mechanisms for air/gases to enter/leave the cage. The cracks at the side of the cage were modeled as 6.35e-3m (0.25”) high planar resistances, with the loss coefficient for these resistances having been determined from the cage wind tunnel CFD simulations (see volume I section 4.2. 1.2). The top of the cage, which was filtered, was defined as a combination of a planar resistance and a planar source. The determination of the loss coefficient for the resistance, and the coefficient for the source has been outlined (see volume I, section 4.2. 1.1). The spacing of the cages on the shelves was dependent on whether the racks were single density (7 cages per shelf), or double density (14 cages per shelf). In the single density cases, the cages were centrally located in the short dimension, and equally spaced in the long dimension. The spacing was 4.88e-2m (1.92”) from comer of cage to comer of adjacent cage. In the double density racks, the cages were equally spaced in both the long and short dimensions. The spacing was 2.20e-2m (0.87”) and 4.88e-2m (1.92”), respectively. Change Station Model Two alternative change stations were considered in this project. Both stations were constmcted primarily from rectangular blocks and triangular prisms. The internal stmcture and flow field were of no concern in this project. It was only the effect of the station on the room airflow that is of importance. The first design is shown in figure 1.03. The station had overall dimensions of 1.32m (52”) x 0.86m (34”) x 1.83m (72”). This design was effectively passive in terms of direct flow field interaction. In particular, the station internally recirculated a flow of 350 cfm (1.65e-l m^ /s). Volume II - 6 Ventilation Desien Handbook on Animal Research Facilities Usin 2 Static Microisolators with only 10 percent leakage defined at the sash opening. The makeup air intake for this leakage was mounted at the side of the station. The station dissipated heat that was expected to affect the room’s overall flow field. In particular, the station contributed a load of 720W to the room. This heat was mostly confined to the lower portion of the station where the motor was located. Figure 1.03 Original Change Station Design Due to concerns raised over the relative passiveness of the first station design, a second design was considered, as is displayed in figure 1 .04. The station had overall dimensions of 1 .50m (59”) X 0.86m (34”) x 1.93m (76”) high. This station also recirculated air, at 300 cfm (1.42e-l m^ /s), but discharged a much higher percentage than the first design. In particular, 200 cfm (9.44e-2 m^/ s) was discharged through grilles at the top of the station. The air makeup to compensate for this discharge was mounted at the front sill at the opening to the station. The station dissipated 660W, and this heat was considered to be added to the air discharge at the top of the station. Volume II - Section I - Introduction Page II - 7 Figure 1.04 Alternative Change Station Design Modeling Assumptions • Solar load was not modeled through the walls of the room. • The floor, ceiling, and walls were assumed to have no heat transfer, i.e., the surrounding areas were assumed to be at the same temperatures. • All surfaces were treated as smooth when calculating surface friction. • The sink in the animal room was modeled as a single rectangular block. The recess formed by the shape of the sink was not modeled because the effect of the recess would be negligible on the overall flow field within the room. • No lighting was assumed in the animal room simulations. The reason for this was that higher CO 2 and NH3 emissions occur during dark (scotophase) conditions (see volume I, section 4. 1.2.8. 1 and appendix I, sections 3.4. 1.9 and 3.4.1.10). Volume II - 8 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators • The animal room was intended to be kept at 22.2 °C (72.0 °F), with temperature control performed on the exhaust air temperature. • No leakage occurred into or out of the animal room other than that specified though the cracks around the door. • Air density variations due to temperature were negligible. Density variation was therefore ignored in all terms apart from in the momentum term for the vertical velocity component. This is known as the Boussinesq approximation. • The levels of CO 2 and NH3 were so diluted in the whole room simulations, even at their source, that the variation of the mixture density due to differing molecular weights was negligible. 1.2 Whole Room Configurations To investigate a range of parameters, the basic model described in section 1.1 was modified. Wherever possible, only one parameter was varied at a time to fully assess the effect. The list of room runs as they were considered is given in table 1.01. Parameters considered in this report are as follows: • Supply Diffuser Type: Three different diffuser types were considered in this project: radial diffusers, low induction diffusers, and slot diffusers. While all these diffuser types are ceiling mounted diffusers, the flow patterns resulting from them are fundamentally different. See volume I, section 4.2.2 and figures 1.05 to 1.07). Volume II - Section I - Introduction Page II - 9 Figure 1.05 Radial Dijfuser Figure 1.06 Slot Dijfuser Volume II - 10 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Figure 1.07 Low Induction Dijfuser • Exhaust Location and Number. Four different exhaust locations were considered: ceiling; high level, low level on the long walls, and low level on the door wall. The number of exhausts used in each of these locations was also considered as a parameter. See figures 1 .08 to 1.11. Volume II - Section I - Introduction Paee II - 11 Figure 1.08 Ceiling Level Exhausts Figure 1.09 High Level Exhausts Volume II - 12 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Figure 1.10 Low Level Exhausts Figure 1.11 Low Level Exhausts on the Door Wall • Room Air Change Rate: addition to the basecase ACH of 15, the following three other ACH values were considered; 5, 10, and 20 ACH. Volume II - Section 1 - Introduction Page II - 13 • Change Station Design and Status: Two different change station designs were considered, as outlined above. The two designs were intended to present both a passive and intrusive influence on the room volume. In the case of the former design, the station was also considered switched off. • Pressurization of Room Relative to Corridor: The pressurization of a room is dependent on the difference between the supply and exhaust flow rates. A higher exhaust rate than supply leads to negative pressurization of the room; hence, makeup air is supplied to the room via available transfer mechanisms, namely door cracks. In this project, the amount of makeup air allowed through the door cracks was varied between 100 cfm (4.72e-l mVs) into the room to 100 cfm (4.72e-l mVs) out of the room. • Orientation of Cage Racks in Room: The racks were considered both parallel to the long walls, and perpendicular to them. See figures 1.12 and 1.13 Figure 1.12 Racks Parallel to Side Walls Volume II - 14 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Figure 1.13 Racks Perpendicular to Side Walls • Side Cracks of Cage Sealed Instead of Open: A series of cases was considered in which the side cracks of the cage were sealed, leaving the cage filter top as the only transfer mechanism. The main objective was to establish the relative increase in cage NH3 and CO 2 levels when the secondary transfer mechanism was removed. • Density of Cages: The number of cages per rack was considered as a parameter. In addition to the basecase value of 42 cages per rack, a double density 84 cages per rack was also considered, as was a reduced density of 28 cages per rack. See figures 1.14 and 1.15. Volume II - Section I - Introduction Page II - 15 Figure 1.14 Double Density Rack Figure 1.15 Reduced Density Rack • Location of Change Station: The location of the change station was swapped with each of the racks in the room. See figures 1.16 to 1.20. Volume II - 16 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Figure 1.17 Change Station Swapped with Rack 2 Volume II - Section I - Introduction Page II - 17 Figure 1.18 Change Station Swapped with Rack 3 Figure 1.19 Change Station Swapped with Rack 4 Volume II - 18 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Figure 1.20 Change Station swapped with Rack 5 • Rack Grouping: In some series, the racks were considered to be along one wall only, rather than spread out through the room. See figure 1.21. Figure 1.21 Racks on One Wall Only • Room Width: Three runs were performed with the room width increased to 4.26m (14’ 0”). Volume II - Section 1 - Introduction Page II - 19 • Supply Air Temperature: In a few runs the supply air temperature was raised to 22.2 °C (72.0 °F), at 49 percent RH which would cause the room and exhaust temperatures to be higher than the design value of the baseline case. Volume II - 20 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 1.01 List of All Whole Room Runs Case Name Supply Diffuser Type Exhaust Location and Number Change Station (Design/ Status) Rack orientation Rack density Pressure of Room to Corridor Supply Temperature °C (“F) Supply ACH Basecase Radial Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 18.8 (65.8) 15 Case 02 Radial High (x4) Thoren/ ON Parallel Single Neg, lOOcfm 18.8(65.8) 15 Case 03 Radial Low (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 04 Slot Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 05 Slot High (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 06 Slot Low (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 07 Low Ind Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 08 Low Ind High (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 09 Low Ind Low (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 10 Radial Ceiling (x2) Thoren/ OFF Parallel Single Neg. lOOcfm 20.7 (69.3) 15 Case 11 Radial Low (x4) Thoren/ OFF Parallel Single Neg. lOOcfm 20.7 (69.3) 15 Case 12 Slot Ceiling (x2) Thoren/ OFF Parallel Single Neg. lOOcfm 20.7 (69.3) 15 Case 13 Slot Low (x4) Thoren/ OFF Parallel Single Neg. lOOcfm 20.7 (69.3) 15 Case 14 Low Ind Ceiling (x2) Thoren/ OFF Parallel Single Neg. lOOcfm 20.7 (69.3) 15 Case 15 Low Ind Low (x4) Thoren/ OFF Parallel Single Neg. lOOcfm 20.7 (69.3) 15 Case 16 Radial Ceiling (x2) Thoren/ ON Parallel Single Pos. lOOcfm 18.8(65.8) 15 Case 17 Slot Ceiling (x2) Thoren/ ON Parallel Single Pos. lOOcfm 18.8(65.8) 15 Case 18 Low Ind Ceiling (x2) Thoren/ ON Parallel Single Pos. lOOcfm 18.8(65.8) 15 Case 19 Radial Ceiling (x2) Thoren/ ON Perpendicular Single Neg. lOOcfm 18.8(65.8) 15 Case 20 Slot Ceiling (x2) Thoren/ ON Perpendicular Single Neg. lOOcfm 18.8(65.8) 15 Case 21 Low Ind Ceiling (x2) Thoren/ ON Perpendicular Single Neg. lOOcfm 18.8(65.8) 15 Case 22 Radial Low (x4) Thoren/ ON Perpendicular Single Neg. lOOcfm 18.8(65.8) 15 Case 23 Slot Low (x4) Thoren/ ON Perpendicular Single Neg. lOOcfm 18.8(65.8) 15 Case 24 Low Ind Low (x4) Thoren/ ON Perpendicular Single Neg. lOOcfm 18.8(65.8) 15 Case 25 * Radial Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 26 * Slot Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 18.8 (65.8) 15 Case 27 * Low Ind Ceiling (x2) Thoren/ ON Parallel Single Neg. KXlcfm 18.8 (65.8) 15 Case 28 Low Ind Low (x4) Thoren/ ON Parallel Single Neg. lOOcfm 16.8(62.2) 10 Case 29 Low Ind Low (x4) Thoren/ ON Parallel Single Neg. lOOcfm 11.0(51.8) 5 Case 30 Low Ind Low (x4) Thoren/ ON Parallel Single Neg. KXlcfm 19.8(67.6) 20 Case 31 Radial Low (x4) Thoren/ ON Perpendicular Double Neg. lOOcfm 17.5 (63.5) 15 Case 32 Slot Low (x4) Thoren/ ON Perpendicular Double Neg. lOOcfm 17.5(63.5) 15 Case 33 Low Ind Low (x4) Thoren/ ON Perpendicular Double Neg. KXlcfm 17.5(63.5) 15 Case 34 Radial Ceiling (x2) Thoren/ ON Perpendicular Double Neg. lOOcfm 17.5(63.5) 15 Case 35 Slot Ceiling (x2) Thoren/ ON Perpendicular Double Neg. lOOcfm 17.5(63.5) 15 Case 36 Low Ind Ceiling (x2) Thoren/ ON Perpendicular Double Neg. lOOcfm 17.5 (63.5) 15 Case 37 Radial Ceiling (xl) / Low (x4) (Mass flow in 50/30 split) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 38 Slot Ceiling (xl) / Low (x4) (Mass flow in 50/50 split) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 39 Low Ind Ceiling (xl) / Low (x4) (Mass flow in 50/50 split) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 40 Radial Ceiling (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 41 Slot Ceiling (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 42 Low Ind Ceiling (x4) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Volume II - Section I - Introduction Page n - 21 Case Name Supply Diffuser T)rpe Exhaust Location and Number Change Station (Design/ Status) Rack orientation Rack density Pressure of Room to Corridor Supply Temperature °C (T) Supply ACH Case 43 Lx)w Ind (rotated by 90°) Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 18,8 (6S.8) 15 Case 44 Radial (rotated by 90°) Ceiling (x2) Tboren/ ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 45 Radial Ceiling (x2) Thoren/ ON Parallel Single Neg, SOcfm 18.8 (6S.8) 15 Case 46 Radial Ceiling (x2) Thoren/ ON Parallel Single Neutral 18.8 (6S.8) 15 Case 47 Radial Ceiling (x2) Thoren/ ON Parallel Single Pos. SOcfm 18.8 (6S.8) 15 Case 48 Radial Ceiling (x2) Thoren/ ON (swapped with rackl ) Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 49 Radial Ceiling (x2) Thoren/ ON (swapped with rack2) Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 50 Radial Ceiling (x2) Thoren/ ON (swapped with rack3) Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 51 Radial Ceiling (x2) Thoren/ ON (swapped with rack4) Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 52 Radial Ceiling (x2) Thoren/ ON (swapped with racks) Parallel Single Neg, lOOcfm 18.8 (6S.8) 15 Case 53 Radial Ceiling (x2) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 54 Slot Ceiling (x2) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 55 Low Ind Ceiling (x2) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 56 Radial High (x4) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 57 Radial Low (x4) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 58 Slot High (x4) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 59 Slot Low (x4) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8 (6S.8) 15 Case 60 Low Ind High (x4) Lab. Ihod. / ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 61 Low Ind Low (x4) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 62 Low Ind Low (x4) Thoren/ ON Perpendicular Double Neg. lOOcfm 6.6 (43.9) 5 Case 63 Low Ind Low (x4) Thoren/ ON Perpendicular Double Neg. lOOcfm 14.8(58.6) 10 Case 64 Low Ind Low (x4) Thoren/ ON Perpendicular Double Neg. lOOcfm 18.9 (66.0) 20 Case 65 Low Ind Low (x4) Lab. Prod. / ON Perjjendicular Double Neg. lOOcfm 17.5 (63.5) 15 Case 66 Low Ind Low (x4) Lab. Prod. / ON Perpendicular Double Neg. lOOcfm 6.6 (43.9) 5 Case 67 Low Ind Low (x4) Lab. Prod. / ON Perpendicular Double Neg. lOOcfm 14.8(58.6) 10 Case 68 Low Ind Low (x4) Lab. Prod. / ON Perpendicular Double Neg. lOOcfm 18.9 (66.0) 20 Case 69 Radial High (x4) / Low (x4) (Mass flow split evenly amongst exhausts) Thoren/ ON Parallel Single Neg. l(X)cfm 18.8(65.8) 15 Voiume II - 22 Ventilation Design Handbook on Animal Research Facilities Using Static Microisoiators Case Name Supply Diffuser Type Exhaust Location and Number Change Station (Design/ Status) Rack orientation Rack density Pressure of Room to Corridor Supply Temperature °C(T) Supply ACH Case 70 Slot High (x4) / Low (x4) (Mass flow split evenly amongst exhausts) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 71 Low Ind High (x4) / Low (x4) (Mass flow split evenly amongst exhausts) Thoren/ ON Parallel Single Neg. KXlcfrn 18.8(65.8) 15 Case 72 Radial High (x4) / Low (x2) (Mass flow split evenly amongst exhausts) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 73 Slot High (x4) / Low (x2) (Mass flow split evenly amongst exhausts) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 74 Low Ind High (x4) / Low (x2) (Mass flow split evenly amongst exhausts) Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 75 Radial Ceiling (xl) / Low (x4) (Mass flow in 50/50 split) Lab. Prod. / ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 76 ** Radial Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 19.2 (66.6) 15 Case 77 ** Slot Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 19.2(66.5) 15 Case 78 Low Ind Ceiling (x2) Thoren/ ON Parallel Single Neg. lOOcfm 19.2(66.6) 15 Case 79 Radial 2 Door exhausts Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 80 Slot 2 Door exhausts Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 81 Low Ind 2 Door exhausts Tlioren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 82 Radial 2 Door exhausts Thoren/ ON Parallel Single Neg. lOOcfm 18.8(65.8) 15 Case 83 Slot 2 Door exhausts Thoren/ ON Perpendicular Double Neg. lOOcfm 17.5 (63.5) 15 Case 84 Low Ind 2 Door exhausts Thoren/ ON Perpendicular Double Neg. lOOcfm 17.5 (63.5) 15 Case 85 Radial Low (x4) Lab. Prod. / ON Perpendicular Double Neg. lOOcfm 17.5 (63.5) 15 Case 86 Slot Low (x4) Lab. Prod. / ON Perpendicular Double Neg. lOOcfm 17.5(63,5) 15 Case 87 Radial Low (x4) Thoren/ ON Parallel Reduced Neg. lOOcfm 19.2(66.6) 15 Case 88 Slot Low (x4) Thoren/ ON Parallel Reduced Neg. lOOcfm 19.2(66.6) 15 Case 89 Low Ind Low (x4) Thoren/ ON Parallel Reduced Neg, lOOcfm 19.2(66.6) 15 Case 90 Radial Ceiling (x2) Thoren/ ON Perpendicular all 5 on 1 wall Single Neg. lOOcfm 18.8(65.8) 15 Case 91 Slot Ceiling (x2) Thoren/ ON Perpendicular all 5 on 1 wall Single Neg. lOOcfm 18.8(65.8) 15 Case 92 Low Ind Ceiling (x2) Thoren/ ON Perpendicular all 5 on 1 wall Single Neg. lOOcfm 18.8(65.8) 15 Volume II - Section I - Introduction Page n - 23 Case Name Supply Diffuser Type Exhaust Location and Number Change Station (Design/ Status) Rack orientation Rack density Pressure of Room to Corridor Supply Temperature °C(“F) Supply ACH Case 93 Radial Ceiling (x2) Thoren/ ON Perpendicular all 5 on 1 wall Double Neg. lOOcfm 17.5(63.5) 15 Case 94 Slot Ceiling (x2) Thoren/ ON Perpendicular all 5 on 1 wall Double Neg. lOOcfm 17.5(63.5) 15 Case 95 Lx)w Ind Ceiling (x2) Thoren/ ON Perpendicular all 5 on 1 wall Double Neg, lOOcfm 17.5 (63.5) 15 Case 96 Radial Ceiling (x2) Lab, Prod. / ON Perpendicular all 5 on 1 wall Double Neg. lOOcfm 17.5 (63.5) 15 Case 97 Slot Ceiling (x2) Lab. Prod. / ON Perpendicular all 5 on 1 wall Double Neg. lOOcfm 17.5(63.5) 15 Case 98 Low Ind Ceiling (x2) Lab. Prod. / ON Perpendicular all 5 on 1 wall Double Neg. lOOcfm 17.5(63.5) 15 Case 99 *** Radial Ceiling (x2) Thoren/ ON Parallel Single Neg. l(X)cfm 22.2 (72,0) 15 Case 100 *** Radial Low (x4) Thoren/ ON Parallel Single Neg. lOOcfm 22.2 (72.0) 15 Case 101 *** Low Ind Low (x4) Lab. Prod. / ON Perjjendicular Double Neg. lOOcfm 22.2 (72.0) 5 * Sealed cages instead of open cages ** Room 4.26m (14’ 0”) wide instead of 3.66m (12’ 0”) *** Supply air temperature fixed at 22.2 °C (72.0 °F). In all other cases, the exhaust temperature was set to be 22.2 °C (72.0 °F) by setting the supply air temperature appropriately. Volume 11-24 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 1.02 Cases Cross-Referenced Against Supply Diffuser Type Supply Diffuser Type Case Number Radial Basecase, 02-03, 10-11, 16, 19, 22, 25, 31, 34, 37, 40, 44*, 45-53, 56-57, 69, 72, 75- 76, 79, 82, 85, 87, 90, 93, 96, 99-100 Slot 04-06,12-13, 17, 20, 23, 26, 32, 35, 38, 41, 54, 58-59, 70, 73, 77, 80, 83, 86, 88, 91, 94, 97 Low Induction 07-09, 14-15, 18, 21, 24, 27-30, 33, 36, 39, 42, 43*, 55, 60-68, 71, 74, 78, 81, 84, 89, 92, 95,98, 101 * Indicates that diffuser rotated by 90° Table 1.03 Cases Cross-Referenced Against Exhaust Location and Number Exhaust Location and Number Case Number Ceiling (x2) Basecase, 04, 07, 10, 12, 14, 16-21, 25-27, 34-36, 43-55, 76-78, 90-99 High (x4) 02, 05, 08, 56, 58, 60 Low (x4) 03, 06, 09, 1 1, 13, 15, 22-24, 28-33, 57, 59, 61-68, 85-89, 100-101 Ceiling (xl) / Low (x4) (Mass flow in 50/50 split) 37-39, 75 Ceiling (x4) 40-42 High (x4)/ Low (x4) (Mass flow split evenly amongst exhausts) 69-71 High (x4)/ Low (x2) (Mass flow split evenly amongst exhausts) 72-74 2 Door Exhausts 79-84 Volume II - Section I - Introduction Page II - 25 Table 1.04 Cases Cross-Referenced Against Change Station Design and Status Change Station Case Number ON* Basecase-09, 16-101 OFF* 10-15 Thoren Design Basecase -52**, 62-64, 69-74, 76-84, 87- 95,99-100 Laboratory Products Design 53-61, 65-68, 75, 85-86, 96-98, 101 ^Change in status was only considered for Thoren design change station. **Cases 10 to 15 had Thoren design change station switched off. Table 1.05 Cases Cross-Referenced Against Pressurization of Room Relative to Corridor Pressurization of Room to Corridor Case Number Neg. 100 cfm basecase -15, 19-44,48-101 Pos. 100 cfm 16-18 Neg. 50cfm 45 Neutral 46 Pos. 50 cfm 47 Table 1.06 Cases Cross-Referenced Against Pressurization of Room Relative to Corridor Rack Orientation Case Number Parallel basecase - 18, 25-30, 37-61***, 69-82, 87- 89, 99-100 Perpendicular 19-24, 31-36, 62-68, 83-86,101 Perpendicular All 5 on 1 Wall 90-98 *** Cases 48 to 52 had change station swapped with each of five racks. Table 1.07 Cases Cross-Referenced Against Status of Cage Side Cracks Status of Cage Side Cracks Case Number Open basecase - 24, 28-101 Sealed 25-27 Volume II - 26 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 1.08 Cases Cross-Referenced Against Density of Cages in Rack Density of Cages in Rack Case Number Single basecase - 30, 37-61, 69-82, 90-92, 99-100 Double 31-36, 62-68, 83-86, 93-98, 101 Reduced 87-89 Table 1.09 Cases Cross-Referenced Against Room Air Change Rate Room Air Change Rate (ACH) Case Number 5 29, 62, 66, 101 10 28, 63, 67 15 basecase - 27, 31-61, 65, 69-100 20 30, 64, 68 Table 1.10 Cases Cross-referenced Against Room Width Room Width (ft) Case Number 12 basecase -75, 79-101 14 76-78 Table 1.11 Cases Cross-Referenced Against Supply Temperature (1) Supply Temperature Case Number Supply Temperature set such that air is at 22.22°C (72°F) at Exhaust basecase - 98 22.22°C 99-101 Volume II - Section I - Introduction Page II - 27 Table 1.12 Cases Cross-Referenced Against Supply Temperature (2) Supply Temperature Case Number 6.60 62, 66 11.00 29 14.80 63,67 16.80 28 17.50 31-36, 65,83-86,93-98 18.80 basecase -09, 16-27, 37-61, 69-75, 79-82, 90-92 18.90 64, 68 19.20 76-78, 87-89 19.80 30 20.70 10-15 22.22**** 99-101 **** In these cases, the supply air temperature was set to 22.22 °C (72 °F). In all other cases, the exhaust air temperature was set to be 22.22 °C (72 °F) by setting the supply air temperature appropriately. Volume II - Section II - Summary of Results of Whole Room Runs Page II - 29 2. SUMMARY OF RESULTS OF WHOLE ROOM RUNS Discussion of these figures and tables can be found in volume I, section 3.1. Figure 2.01 Mean Room Breathing Zone Temperature Figure 2.02 Mean Cage Temperatures Volume II -30 Ventilation Desien Handbook on Animal Research Facilities Using Static Microisolators Figure 2.03 Mean Room Breathing Zone Relative Humidity (RH) Figure 2.04 Mean Cage Relative Humidity (RH) Volume II - Section II - Summary of Results of Whole Room Runs Page II ■ 31 Figure 2.05 Mean Room Breathing Zone CO 2 Concentration E 2000 : Q. Q. o o 1500 j Figure 2.06 Mean Cage CO 2 Concentrations Volume II -32 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Figure 2.07 Mean Room Breathing Zone NHj Concentration on Day 4 Figure 2.08 Mean Cage NH 3 Concentration on Day 4 Volume II - Section II - Summary of Results of Whole Room Runs Page II - 33 Base case Case 02 Case 03 Case 04 Case 05 Case 06 Case 07 Case Od Case 00 Case 10 Case 11 Case 12 Case 13 Case 14 Case 15 Case 16 Case 17 Case 18 Case 10 Case 20 Case 21 Case 22 Case 23 Case 24 Case 25 Case 26 Case 27 Case 28 Case 20 Case 30 Case 31 Case 32 Case 33 Case 34 Case 36 Case 36 Case 37 Case 38 Case 39 Case 40 Case 41 Case 42 Case 43 Case 44 Case 45 Case 46 Case 47 Case 48 Case 49 Case 50 Case 51 Case 52 Case S3 Case 54 Case 55 Case 56 Case 67 Case 56 Case 59 Case 60 Casa 61 Case 62 Case 63 Case 64 Case 65 Case 66 Case 67 Case 68 Case 69 Case 70 Case 71 Case 72 Case 73 Case 74 Case 75 Case 76 Case 77 Case 78 Case 76 Case 80 Case 81 Case 82 Case 83 Case 84 Case 85 Case 86 Case 67 Case 88 Case 86 Case 90 Case 91 Case 92 Case 93 Case 94 Case 95 Case 96 Case 67 Case 96 Case 99 Case 100 Case 101 1 2 0.02 0.04 0.03 0.07 0.03 0.06 0.03 0.06 003 0.05 0.04 0.07 0.03 0.06 0.03 0.06 0.05 0.06 0.02 0.03 0.03 0.05 0.01 0.02 0.02 0.04 0.03 0.05 0.03 0.05 0.02 0.04 0.04 0.08 0.04 0.08 0.03 006 0.04 0.08 0.03 0.06 0.03 0.06 0.03 0.06 0.04 0.08 0.03 0.06 0.02 0.04 0.03 0.06 0.06 0.11 0.07 0.14 0.03 0.06 0.06 0.11 0.06 0.13 0.07 0.14 0,07 0,14 0.06 0.12 0.07 0.14 0.03 0.06 0.03 0.06 0.04 0.07 001 0.02 0.03 0.05 0.03 0.06 0.03 0.07 0.04 0.07 0.03 0.05 0.02 0.04 0.02 0.04 0.03 0.07 0.03 0.05 0.03 0.06 0.04 0.07 0.03 0.07 0.03 0.06 003 0.06 0.03 0.06 0.04 0.07 0.03 0.06 0.04 0.07 0.01 0.01 0.03 0.06 0.04 0.07 0.16 0.35 0.11 0.22 0.05 0.09 0.07 0.14 0.17 0.32 0.11 0,20 0.05 0.09 0.03 0.05 0.01 0.02 0.03 0.05 0.03 0.05 0.02 0.04 0.02 0.05 0.03 0.05 0.03 0.06 0-03 0.05 0.03 0.06 004 008 0.05 0.09 0.05 0.09 0.(X 0.12 0,09 0.17 0.06 0.15 0.07 0.13 0.06 0.13 0.02 0.05 0.02 0.05 0.03 0.06 0.04 0.07 0.03 0.06 0.02 0.04 0.05 0.09 0.06 0.12 0 04 0.07 0.05 0.10 0.06 0.11 0.04 006 0.01 0.03 0.02 0.04 0 15 0.29 Day by day room breathing zona average NH3 3 4 5 6 7 6 9 0.06 0.10 0.10 0.10 0.08 0.11 0.09 0.10 0.14 0.05 0.07 0.03 0.08 0.06 0.07 0 06 0,12 0.12 0.00 0.12 0.10 0.10 0.10 0.13 0.09 0.05 0.10 0.18 0.22 0.10 0.17 0.10 0.22 0.21 0.19 0.21 0.09 0.10 0.11 0.03 0.08 0.10 0.10 0.11 0.06 0.07 0.06 0.10 0.06 0.09 0.10 0.10 0.09 0.09 009 0.11 0.09 0.11 0.02 0.09 0.11 0.53 0.34 0.15 0.21 0.50 0,31 0.15 0.08 0.04 0.08 0.07 0.06 0.07 0.07 0.09 0.08 0.09 0.13 0.14 0.14 0.18 0.26 0.24 0.21 0.19 0.07 0.07 0.10 0.11 0.09 0.06 0.13 0.18 0.11 0 16 0.17 0.13 0.04 0.06 0.44 0.11 0.15 0.14 0.15 0.12 0.16 0.16 0.16 0.20 0.07 0.12 0.04 0.11 0.14 0.11 0.10 0.20 0.20 0.16 0.18 0.16 0.14 0.14 0.16 0.22 0.16 0.24 0.25 0.32 0.14 0.24 0.28 0.32 0.41 0.34 0.39 0.14 0.16 0.16 0.06 0.12 0.16 0.17 0.21 0.14 0.12 0.11 0.16 0.14 0.14 0 18 0 18 0.18 0.17 0.17 0 18 0.13 0.16 0.04 0.18 0.16 0.77 0.58 0.21 033 0.72 0.57 0.21 0,12 0.06 0.12 0.11 0.09 0.11 0.13 0.14 0.13 0.15 0.18 0.20 0.20 0.27 0.49 0.34 0.33 0.29 0.10 0.11 0.14 0.18 0.14 0.10 0.24 0.31 0.18 0.28 0.29 0.23 0.06 0.09 0.64 0.18 0.23 0.20 023 0.16 0.23 0.27 0 26 0.28 0,10 0 19 0.07 0.18 0.21 0.16 0.15 0.32 0.32 0.25 0.27 0.26 0.20 0.20 0.26 0.41 0.32 0.45 0.36 0.44 0.20 0.35 0.39 0.45 0.69 0.56 0.65 0.21 0.22 0.27 0.09 0.16 0.23 0.26 0.35 0.23 0.19 0.16 0.30 0.23 0.22 0.30 0.28 0.32 0.28 0.30 0.29 0.16 0.22 0.07 0.32 0.23 1.08 0.94 0.29 0.49 1.01 0.93 0.29 0.16 0.08 0.17 0.17 0,14 0.17 0.21 0.23 0.21 024 026 0.29 0.28 0.40 0.82 0-48 0.49 0.43 0.14 0.16 0.20 0.26 0.22 0.15 0.36 0 49 0.29 0 46 0.46 0 39 0.09 0.13 0.90 0.26 0.32 0.26 0.32 0.25 0.31 0.41 0.37 0.36 0.14 0.27 0.10 0.26 0.31 0.23 0 22 046 0.46 0.36 0.39 0.37 0.27 0.28 036 0.63 0.51 0.70 0.50 062 0.28 0.49 0.55 0 63 1.03 0.82 0.96 0.30 0.32 0.38 0.13 0.26 0.33 0.40 0.61 0.33 0.28 0.26 0.45 0.34 0.32 0.44 0.41 0.49 0.42 0.45 0.42 0.25 0.31 0.11 048 0.32 1.50 1.37 0.41 0.70 1.40 1.37 0.41 0.22 0,11 0,24 0.23 0.20 0.24 0.30 0.33 0.30 0.35 0.36 0.40 0.39 0.66 1.22 0,67 0,70 0.61 0.20 0.21 0.27 0.40 0.32 0.22 0.66 0.71 0.41 0.68 0.57 0.57 0.12 0.18 1.25 0.40 0 45 0.38 0.46 0.38 0.43 0.61 0.54 0.53 0.19 0.39 0.14 0.39 0.45 0.32 0.31 0.66 0.87 0.54 0,55 0.54 0.37 0.38 0.49 0.99 0.81 1.10 0.66 0.94 0.38 0.68 0.75 0.86 1.55 1.21 1.44 0.42 0.45 0.65 0.20 0.36 0.46 0.59 0.76 0.49 0.42 0.38 0.67 0.50 0.46 0,65 0.60 0.74 0.62 0.63 0.62 034 0.43 0.16 0.74 0.45 2.05 2.02 0^8 0.99 1.91 2.04 0.56 0.31 0.16 0.34 0.33 0.28 0.34 0.45 0.48 0.44 0.51 0.49 0.54 0,54 0.79 1.84 0.91 1.00 0,85 0.27 0.29 0.37 0.57 0.48 0.31 0.82 1.04 0.60 1.01 0.98 0.35 0.16 0.24 1.71 0.49 0.59 0.52 0.60 0.47 0.53 0.76 0.69 0.71 0.26 0.51 0.18 0.49 0.58 0.42 0.40 0.85 0.85 0.68 0.72 0.69 0.50 0.51 0.66 1.19 0.9S 1.31 0.92 1.14 0.52 0.90 1.01 1.16 1.92 1.52 1.79 0.55 0.59 0.71 0.25 0.47 0.62 0.75 0.95 0.62 0.53 0.48 0.S4 0.63 0.59 0.82 0.76 0.91 0.78 0.84 0.79 0.46 0.58 0.20 0.90 0.59 2.77 2.55 0.75 1.30 2.58 2.55 0.75 0.41 0.21 0.45 0.43 0.37 0.45 0,57 0.61 0.56 0.64 0.66 0.73 0.72 1.04 2.28 1.23 1.30 1.13 0.36 0.39 0.50 0.74 0.59 0.41 1.04 1.33 0.77 1.27 1.25 1.06 0.22 0.32 2.30 0.59 0.76 0.69 0.77 0.60 0.77 0.91 0.86 0.94 0.33 0.63 0,23 0.59 0.72 0.55 0.50 1.06 1.06 0.84 0.92 0.85 0.66 0.60 0.88 1.36 1.05 1.40 1.22 1,51 0.68 1.19 1.34 1.54 2.29 1.85 2.15 0.71 0.75 0.90 0.31 0.81 0.79 0.93 1.15 0,76 0.64 0.58 1.01 0.77 0.74 1.00 0.94 1.07 0.94 0.99 0.98 0.62 0.76 0.24 1.05 0.78 3.66 3.13 1.00 1.65 3.42 3.10 1.00 0.55 0.28 0.58 0.56 0.47 0.57 0.69 0.76 0.70 0.79 0.87 0.97 0.96 1.35 2.73 1.64 1.65 1.46 0.46 0.51 0.67 0.93 0.75 0.52 1.27 1.64 0.96 1.54 1.54 1.29 0.29 0.43 3.06 10 0.63 0.95 0.90 0.94 0.74 1.01 0.99 1.01 1.23 0.42 0.75 0.27 0.64 0.83 0.70 0.58 1.23 1.25 0.96 1.13 0.99 0.66 0.89 1.15 1.30 0.92 1.39 1.59 1.97 0.89 1.52 1.75 2.01 2.44 2.06 2.34 0.07 0.93 1.08 0.35 0.75 0.96 1.06 1.27 0.86 0.72 0.66 1.11 0.86 0.68 1.12 1.07 1.10 1.03 1.04 1.12 0.61 0.97 0.26 1.08 0.09 4.81 3.54 1.31 2.01 4.47 3.42 1.31 0.72 0.35 0.74 0.70 0.56 0.71 0.79 0.68 0.81 0.90 1.14 1.27 1.25 1.70 2.94 2.14 2.00 1.82 0.63 0.67 0.87 1.11 0.69 0.63 1.43 1.89 1.11 1.70 1,79 1.42 0.38 0.56 4.00 1 2 0-07 0.14 0.09 0.18 0.09 0.17 0.09 0.17 0.06 0.16 0.08 0.16 0.20 0.38 0.12 0.23 0.14 0.28 0.08 0.16 0.12 0.23 0.12 0.23 0.15 0.28 0.12 0.22 0.16 0.31 0.13 0.25 0.10 0.18 0.10 0.18 0.12 0.22 0.09 0.16 0.10 0.19 O.n 0.22 0.09 0.16 0.22 0.43 0.13 024 0.08 0.16 0.13 0.24 0.16 0.31 0.14 0.27 0.13 0.25 0.16 0.32 0.18 0.34 022 0.44 0.17 0.32 0.15 0.29 0.16 0.31 0 09 0.18 0.10 0.20 0.13 0.25 0.05 0.09 0.07 0.14 0,10 0.19 009 0,17 0.13 0.24 0.13 0.25 0.11 0.20 0.09 0.18 0.16 0.31 0.13 0.25 0.10 0.20 0.12 0.23 0.19 0.37 0.09 0.17 0.15 0.29 0.13 0.24 0.08 0.16 0.15 0.26 0.13 0.26 0.05 0.09 0.09 0.16 0.16 0.31 0.47 0.91 0.34 0.66 0.17 0.33 0,17 0.32 0.43 0.83 0.33 0.63 0.14 0,26 0.14 0.26 0 06 0.12 0.16 0.31 0.13 0.26 0-08 0.15 0.13 0.25 0.13 0.25 0.12 0.24 0.10 0.19 0.11 0.21 0.10 0.20 0.11 0.21 0.14 0.28 0.17 0.34 0.21 0-41 0.20 0.38 0.23 0.45 0.20 0.38 0.07 0.14 0.04 0.09 0.11 0.22 0.15 0.28 0.10 0.19 0 06 0 IS 0.19 0.37 0.17 0.33 0.15 0.28 0.15 0.28 0.15 0-30 0.18 034 0,06 0.12 0.10 0.19 0.41 0.60 Day by day room breathing zone maximum NH3 3 4 5 6 7 8 9 0.21 0.26 0.27 0.26 0.24 0.25 0.58 0.35 0.43 0.25 0.35 0.36 0.44 0.34 0.48 0.39 0.28 0.28 0,35 0.27 0.29 0.34 0.28 0.67 0.36 0.23 0.37 0.46 0.42 0.39 0.49 0.53 0.67 0.50 0.45 0 47 0.28 0.31 0.39 0.13 0.22 0.30 0.26 0.37 0.36 0.31 0.27 0,47 0.38 0.31 0.38 0.S7 0.26 0.44 0.36 0.26 0.44 0.40 0,14 0.27 0.48 1.40 1.01 0.51 0.50 1.28 0.97 0.41 0.41 0.19 0.48 0 40 0.23 0.39 0.39 0.37 0.30 0.33 0.30 0.32 0.43 0.52 0.63 0.59 0.69 0.58 0.21 0.13 0.34 0.44 0.29 0.24 0.56 0.51 0.44 0.44 0.46 0.52 0.19 0.30 1.24 0.40 0,43 0.38 0.42 0.37 0.35 1.07 0.53 062 0 37 0.57 0.59 0,81 0.58 0.71 0.63 0.47 0.47 0.59 0.43 0.49 0.49 0.41 0.96 0.36 0.69 0.93 0.69 0.60 0,57 0.72 0.76 0,97 0.95 0.81 0.38 0.43 0.47 0.62 0.23 0.34 0.47 0.44 0.67 0,67 0.55 048 0.86 0.68 0.51 0,63 0.98 0.53 0.81 0,74 0.42 0.63 0.58 0.27 0.56 0.72 2.02 1.76 0.73 0.79 1.84 1.75 0.59 0.59 0.29 0.71 0.60 0.36 0.59 0.67 062 0,50 0.57 0.44 0.47 0.63 0.78 1.18 0.85 1.10 0.89 0.30 0.19 0.49 0.70 0.46 0.37 0.98 0.87 0.74 0,79 0.77 0.94 0.28 0.43 1.78 0.63 0.63 0,54 0.62 0.56 0.50 1.79 0.91 0.38 0.53 0.38 0.92 1.36 0 91 1.03 1.02 0.73 0.73 0.95 0.64 0,77 0 69 0.57 1.35 1 60 1.39 1.77 0,97 0.35 0.80 1.04 1.07 1.37 1.61 1.32 1.47 0.64 0.70 0,94 0.37 0.50 0.70 0.70 1.11 1.07 0.89 0.78 1.42 1.10 0.79 1.03 1.57 0.93 1.33 1.26 0,66 088 0.84 0.45 0.99 1.03 2.64 2.83 1.02 1.18 2.59 2.86 0.83 0.32 0.42 1.02 0.87 0.55 0.88 1.08 0.97 0.79 0.91 0.62 065 0.68 1.14 1.99 1.20 1.66 1.30 0.42 0.27 0.69 1.08 0.71 0.66 1.59 1.38 1.16 1.30 1.22 1.54 0.39 0,61 2.51 1.01 0.88 0.75 0.89 0.79 0.69 2.65 1.31 1.21 0.75 1.27 1.32 2.01 1.32 1.45 1.48 1.06 1.05 1.38 0.91 1.11 0.97 0.79 1.87 245 2.16 2-72 1.35 1.17 1.10 1.45 1 48 1.90 2.40 1.95 2.18 0.90 1.00 1.34 0.54 0.70 1.00 1.01 1.65 1.56 1.31 1.14 2.10 1.61 1.14 1.51 2.29 1.40 1.97 1.89 0.96 1.22 1.17 0.67 1.48 1.45 3.94 4.14 1.42 1.68 3.59 4.25 1.15 1-14 0.58 1.43 1.23 0.78 1.25 1.57 1.40 1.14 1.33 0.86 0.91 1.22 1.61 2.95 1.67 2.36 1.83 0.69 0.38 0.95 1.55 1.03 0.60 2.33 2.01 1.68 1.91 1.77 2.28 0.54 0.84 348 1.51 1.24 1.02 1.27 1.12 0.94 3.97 1.90 1.66 1.05 1.34 1.92 3.02 1.93 2.02 2.16 1.54 1.53 2.03 1.29 1.62 1.33 1.08 2.56 3-83 3.50 4.29 1.84 1.60 1.51 2.00 2.02 2.60 3.63 2.69 3.26 1.28 1.41 1.91 0.79 0.99 1.43 1.49 2.46 2.30 1.94 1.66 3.14 2.36 1.6S 2.23 3.37 2.14 2.94 2.86 1.41 1,68' 1.62 1.01 2.27 2.01 5.39 6.10 1.94 2.39 4.91 6.32 1.57 1.56 0.82 1.99 1.72 1.12 1.77 2.32 2.04 1.67 1.95 1.17 1.24 1.67 2.25 4.44 2.28 3.37 2.57 0.81 0.51 1.30 2.24 1.48 1.14 3.43 2.94 2.44 2.85 2.59 3.39 0.74 1.16 4.76 1.88 1.64 1.38 1.65 1.47 1.27 4.94 2.43 2.24 1.39 2.36 2.46 3.75 2.45 2.69 2.76 1.97 1.96 2.57 1.68 2.06 1.78 1.46 3.46 4-59 4.10 5.11 2.48 2.18 2.04 2.68 2-73 350 4.49 3.63 4.06 1.66 1,85 2.48 1.00 1.30 1.05 1.69 3.07 2.91 2.44 2.12 3.92 3.00 2.12 2.81 4.26 2.62 3.67 3.53 1.79 2.26 2,16 1.25 2.77 2.68 7.27 7.71 2.62 3.12 6.63 7.92 2-12 2.11 1.08 2.65 2.27 1.45 2.32 2.93 2,61 2.13 2.47 1.56 1.68 2.25 2.97 5.51 3.07 4.38 3.39 1.09 0.69 1.75 2.89 1.90 1.48 4.33 3.73 3.12 3.56 3.29 4.24 1.00 1.56 6.42 2 25 2.12 1.83 2,10 1.88 1.69 5.94 3.04 2.98 1.81 2.96 3.08 4.51 3.04 3.50 3.43 2.46 2.44 3.16 2.15 2.56 2.36 1.94 4.59 5.23 4.52 5.77 3.30 2.66 2.71 3.51 3.63 4.65 5.35 4.41 4.86 2.15 2 38 3.15 1.23 1.68 2.36 2.33 3.71 3.57 2.98 2.59 4,73 3.66 2.65 3.42 5.24 3.08 4.43 4.18 2.21 3.00 2.83 1.49 3.25 3.50 9.65 9.46 3.48 3.97 8.81 9.60 2.81 2.80 1.41 3.46 2.95 1.84 2.96 3.60 3.24 2.64 3.04 2.10 2.23 2.99 3.86 6.60 4.08 6.57 4.39 1.44 0.92 2.33 3.63 2.39 1.88 5.30 4.61 3.87 4.32 4.08 5.14 1.33 2.07 8.53 10 2.43 2.65 2.39 2.56 2.30 2.21 6.46 3.56 3.89 2.29 3.49 3.63 4.90 3.52 4.43 4.00 2.87 2.86 3.62 2.62 2.98 3.06 2.64 6.00 5.01 3.96 5.40 4.31 3.76 3.54 4.51 4.74 6.08 5.70 4.91 5.30 2.66 2.93 3.80 1.40 2.08 2.87 2.66 4.07 4.05 3.34 2.92 5,19 4.11 3.11 3.83 5.95 3.18 4.87 4.40 2.54 3.62 3 63 1.60 3.33 4.47 12.61 10.71 4.55 4.85 11.51 10.01 368 3.66 1.78 440 3.70 2.22 3.68 4.07 3.77 3.06 3.46 2.74 2.91 3.91 4.86 7.11 5.34 6.75 5.49 1.69 1.21 3.04 4.33 2.85 2.29 5.98 5.31 4.49 4.77 4.72 5.68 1.73 2.71 11.15 Table 2.01 Room Breathing Zone NH 3 Mean and Maximum Concentrations for 10 Days Volume II -34 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Basocase Casa 02 Case 03 Case 04 Casa 05 Casa 06 Case 07 Casa 08 Case 09 Case 10 Casa 11 Casa 12 Casa 13 Casa 14 Casa 15 Case 16 Casa 17 Casa 18 Casa 19 Casa 20 Casa 21 Casa 22 Casa 23 Casa 24 Cass 25 Casa 26 Case 27 Casa 25 Casa 29 Case 30 Casa 31 Casa 32 Casa 33 Casa 34 Casa 35 Casa 36 Casa 37 Case 38 Casa 39 Casa 40 Casa 41 Casa 42 Casa 43 Case 44 Casa 45 Casa 46 Casa 47 Casa 48 Case 49 Casa 50 Casa 51 CasaS2 Casa 53 Casa 54 Casa 55 Case 56 Case 57 Cose 56 Casa 59 Case 60 Casa 61 Casa 62 Casa 63 Case 64 Case 65 Casa 66 Casa 67 Casa 66 Casa 69 Case 70 Case 71 Casa 72 Casa 73 Casa 74 Casa 75 Casa 76 Casa 77 Case 76 Case 79 Case 80 Case 81 Casa 62 Case 83 Case 84 Case 85 Case 86 Caoa 67 Case 88 Case 89 Casa 90 Casa 91 Casa 92 Case 93 Casa 94 Casa 95 Casa 96 Casa 97 Casa 98 Casa 99 Casa 100 Casa 101 1.15 2.18 0.89 1.72 0.82 1,59 0.90 1.73 0.91 1.76 0.86 1.67 1.24 2.35 0.96 1.89 0.90 1.75 1.11 2.14 1.11 2.14 1.16 2,23 1.33 2.52 1.17 2.24 1.11 2.14 1.17 2.24 0.96 1.84 0.94 1.81 0.91 1.75 0.87 1.68 0.95 1.63 0.87 1.69 0.86 1.67 0.98 1.91 1.64 3.04 2.25 4.02 1.75 3.21 0.91 1.77 0.92 1.79 0.87 1.69 1.01 1.96 0.91 1.77 1.03 2.00 1.01 1.92 0.93 1.70 1.00 1.90 0.90 1.74 0.93 1.80 0.97 1,87 1.04 2.00 0.88 1.71 0.91 1.75 0.98 1.88 1.13 2.14 1.02 1.95 1.03 1.97 1.04 1.98 1.12 2.12 1.07 2.05 0.97 1,86 1.08 2-07 0.68 1.69 1.19 2.24 0.99 1.89 1.09 2.06 1.00 1.91 0.96 1.66 1.04 2.01 1.15 2.19 1.27 2.38 1.06 2.07 1.21 2.35 1.10 2.11 0.94 1.83 1 .05 2.03 1.21 2.35 1.14 2.17 0.96 1.66 0.91 1.77 1.02 1.98 1.03 1.99 0.93 1.81 1.03 1.99 0.99 1.91 0.97 1 86 0.98 1.86 0.99 1.90 1.01 1.94 0-86 1.67 0,89 1.73 0.91 1.76 0.87 1.69 0.94 1.78 0.96 1.88 1.01 1.95 1 .00 1 .94 0.72 1.41 0.81 ^St 0.65 1.66 0 68 1 69 0.90 1.73 0 86 1.65 0 96 1.84 0.93 1.78 0.96 1.84 1.04 1.98 0.91 1.75 1.04 1.98 1.03 2.01 0-90 1.75 1.25 243 3 3.37 2.66 2.45 2.67 2.72 2.56 2.69 3.31 3.30 3.46 3.89 3.46 3.31 3.45 2.83 2.79 2.69 2.58 2.82 2.60 2.SB 2.94 4.69 6.20 4.95 2.73 2.75 2.60 3.02 2.73 3.08 2.96 2.75 2.93 2.68 2.77 2.88 3.08 2.64 2.70 2.90 3.31 3.01 3.03 3.06 3.28 3.16 2.87 3.19 2.60 346 2.92 3.16 2.94 2.89 3.10 3.37 3.67 3.19 3.63 3.2S 2.82 3.12 3.62 3.34 2.67 2.73 3.05 3.07 2.79 3.07 294 2.87 2.90 2.93 2.99 2.57 2.66 2-72 2.60 2.74 2.89 3.01 2.99 2.17 2.42 2.55 2.61 2.67 Z.5S 2.84 2-74 2.84 3.05 2.69 3.05 3.10 2.70 3.74 Day by day cage average NH3 4 S 6 7 8 9 10 6.33 4.03 3.53 4.18 4.23 3.72 6.71 4.83 3.88 4.94 5.42 5.66 7.21 S.B2 4.94 5.76 4.71 4.62 4.61 4.04 4.73 3.77 3.71 4.24 11.05 16.42 12.31 3.93 3.97 3.75 4.45 3.93 4.44 5.66 4.90 5.44 4.13 4.26 4.58 5.33 4 06 4.25 4.96 6.03 5.22 5.35 536 6.98 5.56 4.73 5.64 4.49 7.02 5.32 6.22 5.01 4.17 4.58 6.38 7.56 4.73 5i3 5 64 4.07 4.90 5.21 6.03 4.13 3.94 4.58 4.56 4.22 4.87 4.53 4.96 4.85 4.94 5.16 3.70 3.84 3.92 3.92 5.15 4.17 4.76 4.54 3.13 3.49 3.68 4.22 433 4.02 4.97 4 66 4.77 531 4.54 5.50 4 46 3,89 5.40 10.66 5.92 4.96 6.29 6.32 5.22 1131 7.53 5.46 7.17 8.40 6.78 12.05 9.17 7.17 9.02 7.38 7.19 7.36 6.06 7.44 534 5.22 5.96 20 64 37.17 23 47 5.53 5.58 537 6.37 5.53 6.24 9.63 8.01 9.09 6.14 6.36 6.94 655 6.01 6.41 7-90 997 6 40 8.66 867 9.91 9.02 736 9.17 730 12.29 8.60 10.69 7.95 5,07 6.56 10.78 1331 6 81 736 9.06 5.72 736 733 9.62 5.81 5.54 6 66 6.56 6.15 739 6.72 0.01 7.61 7.78 835 531 5.40 531 5.72 B.S7 5. 86 7.19 6 66 4.40 4.90 5.17 6.48 6.65 6.06 8.02 730 7.50 9.06 7.16 9.05 638 S.47 7.59 15.82 8.35 6.88 8.96 8.99 7.24 16.60 10.88 7.57 10.06 12.10 12.68 17.85 1330 10.06 13.05 10.67 10.39 10.72 8.62 10 79 7.42 734 8.26 31.63 5831 36.19 7.67 7.74 731 8.89 7.66 6.66 14.34 11.77 13-47 0.70 9.02 9.92 12.46 8.51 9.14 1130 14.71 1237 12.72 12.68 14.62 1331 10.62 13.45 1031 1848 12.98 15.97 11.56 8.14 9.16 16.02 20.05 934 1030 1336 7.93 10.48 10.17 14,61 8.06 7.68 9.36 932 8.67 10.57 933 11- 70 11.03 11.29 12.03 732 7.49 7.64 8.06 12- 07 8.13 1036 939 6.10 6.80 7.17 932 937 8 65 11.74 10.73 10 87 13- » 1039 1333 8 70 739 10.53 23.70 11.72 9.41 12.75 12.‘^ 9.92 24.07 16.79 10.36 14.W 1731 26.75 19.40 14.03 16.99 15.52 15.07 15.74 12.26 16.72 10.20 9.91 1130 49.49 93.38 57.03 10.50 10.59 10.01 1230 10.49 11.85 21.65 17.46 20.19 12.31 12.76 14.18 1636 12.02 13.03 16.87 21 96 18.08 18.82 10.73 21.83 1934 15.40 19.61 15-47 28.24 1937 2432 16.93 11.13 12.68 24.13 30.71 1336 13.96 19.55 10.85 14.92 13.91 21.75 11.03 1031 13.08 12.82 12.14 16.12 13-46 17.24 18.07 16.47 17.67 9.68 10.24 10.45 11.28 1034 11.12 14.64 13.19 835 930 9.82 13 41 13.79 12.33 1732 15.71 15.84 19.87 15.18 19.85 11.91 1038 2931 15.45 12.70 16.61 16.66 13.37 30.95 20.22 13.97 18-61 22.48 23.52 3339 24.73 18.61 24.26 19.64 19.30 19.95 15.98 20.05 13.71 13.37 15.25 59.31 109.47 67.01 14.16 14.28 13.50 1644 14.15 16.98 26.77 21.62 25.12 16.12 16.71 1639 2332 15.77 16.94 21.40 27.43 22.64 23.69 23.62 27.26 24.61 10.74 25.05 19.57 34.54 24 30 29 63 2130 16.02 16.03 29.90 37.49 17.64 1633 24 60 14.64 19.44 18.77 27.23 14.89 14.17 17.32 17.06 16.04 10.61 17.te 21.79 2031 20.08 22.38 1333 13 82 14.10 14.91 24.01 1500 19.02 1739 1136 12.55 1335 1739 17.76 1604 21.85 19.97 2031 24 88 19.33 24.85 1607 14 01 16.43 35.35 19.99 16.87 21.17 21.31 17.76 37.22 25.23 18.55 24.25 28.16 29.43 40.02 30.67 24.25 30.19 24.70 24.10 24.59 20.40 24.90 18.13 17.76 20.25 67.69 120.68 76.69 18.81 18.97 17.93 21.59 10.79 21.23 31.90 26.67 30.19 20.70 21.45 23.33 26.53 20.28 21.56 26.38 33.16 28.03 26.92 28.89 32.92 30.06 24.67 30.66 24.04 40.56 29.24 36.36 26.67 19.95 22.23 35.76 43.90 23.00 25.01 30.29 19.44 24.79 24.92 33.01 19.77 18.82 22.53 22.30 20.81 24 66 22 68 26.73 25.49 26.03 27.64 17.71 18.35 18.72 19.35 20.75 19.93 24.18 22.50 14.96 16.67 17.59 21.77 2233 2039 26.75 24.68 26.09 30.15 23.96 30.12 2134 10.61 26.81 38.09 25,01 22.05 25.80 26.14 23.22 40.46 29.56 24.25 30.72 33.21 34.67 43.47 35.51 30.72 35.20 28.62 28.27 26.11 24.94 28.90 23.55 23.22 26.47 64.80 105.66 71.78 24.59 24.80 23.44 27.73 24.57 27.75 34.00 29.69 32.78 25.65 26.46 28.19 32.42 25.10 26.19 30.19 36.40 31.77 32.44 32.55 36.12 33.76 28.99 34.22 27.33 41.81 32.12 37.24 30.53 26.08 26.50 30.37 44.97 29.42 32.69 34.30 25.42 30.23 32.56 36.49 25.84 24.61 28.44 28.39 26.16 30.01 28.03 30.30 26.63 30.16 31.38 2315 23 99 24.47 24.34 30.99 26.05 29.32 26.16 19.55 21.78 23.00 25.93 26.55 24.75 30.10 28.40 29.12 33.32 27.70 33.29 27.89 24.32 33.74 Pay by day cage maximum NH3 1 2 3 4 5 6 7 6 1.67 1.29 1.34 1.20 1.22 1.38 1.80 1.39 1.52 1.67 1.53 1.59 1.60 1.38 1.67 1.65 1.30 1.26 1.24 1.23 1.35 1.37 1.33 1.57 2.15 2.73 2.51 1.41 1.33 1.34 1.46 1.45 1.63 1.44 1.43 1.38 1.17 1.36 1.41 1.47 1.25 1.33 1.28 1.45 1.42 1.45 1.48 1.44 1.42 1.36 1.44 1.39 1.52 1.42 1.40 1.36 1.43 1.50 1.46 1.58 1.47 1.76 1.60 1-36 1.65 1.60 1.81 1.51 134 1.43 1.40 1.39 1.30 1.39 138 1.40 1.39 132 1>40 1>41 1.46 1.61 1.54 1.49 1.70 137 1.31 1.35 132 139 136 1>47 1>44 1.47 1.47 132 1.44 1.64 1.72 1.83 3.17 2.61 2.61 2.32 236 2.68 3.43 2.67 2.97 334 2.94 3.06 3.04 2.65 3.24 3.16 2.46 2.47 2.36 236 2.59 2.66 238 3.06 3.96 4.90 4.61 2.74 2.69 2.61 2.87 2.82 3.17 2.72 2.72 2.63 2.27 2.70 2.72 2.61 2.41 237 2.46 2.77 2.71 2.78 2.64 2.75 2.72 2.63 2.74 2.66 2.86 2.71 2.66 2.60 2.77 2.90 2.77 2.96 2.66 3.43 344 ^65 3.19 331 3.45 2.93 2.61 2.77 2.72 2.69 2.73 2.66 2.65 2.46 2.68 2.66 238 2.72 2.75 2.83 3.06 3.00 2.07 3.29 2.67 235 2.63 2.54 266 2.43 2.81 2.77 2.61 2.80 2.92 2.74 3.00 335 336 4.89 387 4.03 338 3.64 4 13 5.28 4.12 4.57 5.00 4.53 4.72 4.66 4.09 5.00 4.68 384 3.80 3.67 3.67 3.99 4.09 3.98 4 72 6.15 7.55 7.11 4.22 3.99 4.02 4.43 4.35 4.89 430 4.20 4.05 330 4.16 4 19 434 3.71 3.96 3.79 4 26 4.18 4 28 436 4.24 4.19 4.05 433 4.09 4.41 4.18 4.09 401 4.26 4.46 4.27 4.57 4.41 539 5.31 4.00 4.91 5>41 531 4.52 4 03 438 4.19 4.15 4.22 4.11 4.09 3.80 4 14 4.10 3.67 4.19 4.24 4.36 4.72 4.62 4.43 5.07 4.12 3.93 4.05 3.92 4.12 3.7S 4.34 437 434 4.31 4.50 433 4.62 5.16 5.50 0.19 5.07 5.81 5.62 5.67 5.95 9 79 6.81 6.59 7.47 7.43 7.7S 8.69 6.86 7.47 8.13 6.40 6.28 6.29 5.74 6.69 5.94 5 74 6 80 14.47 22 42 17.70 6.08 5.75 5.79 6.52 637 7.05 B.03 7.49 731 5.41 6.42 6.65 7.50 5.72 6.23 6 49 7 77 736 736 7.67 7.73 737 6.67 7.40 7.06 6.96 7.61 8.00 6.82 6.17 6.60 6.09 9.40 6.54 7.62 9.23 6.69 7.70 7.60 936 6.52 5.61 642 632 637 6.70 6.33 7.10 636 6.96 7.05 5.30 6.05 6.12 638 8.87 6 67 7.00 7.70 6.93 6.66 5.85 6.34 6 69 5.92 7.58 734 738 7.78 7.59 7.64 6.66 743 7.92 15.47 8.61 8.16 8.45 6.46 837 16.35 10.62 9.27 10.83 11.51 12.02 14.52 10.84 10.83 12.74 10.01 9,78 10.04 8.00 10.52 6.41 8.07 9.56 27.02 45.23 33.74 8.56 8.08 8.15 9.33 8.62 9.91 13.66 12.24 12.56 8.03 9.54 10 08 12-02 8.47 9.40 10.34 12.66 11.68 12.26 1240 12.81 11.95 1037 12.16 1135 16.70 12.60 13.76 10.84 8.67 9.45 13.66 16.57 9.42 10.72 14.84 636 1137 10.97 15.76 9.17 8.17 6.33 8.97 9.16 10.16 9.39 11-42 9.98 10.97 1138 7.45 8.60 8.60 9 59 14.92 9.37 1037 1138 0.34 7.96 832 9.73 10.29 8.93 1234 11>49 11,44 12.80 11.98 1236 9.36 10.46 11.14 22.96 12.14 11.32 12.03 12.03 11.60 24.20 15 34 12.86 15.20 16.59 17.33 21 .50 15.72 15.20 18.43 14.46 14.12 14.63 12.25 15.24 11.69 11.16 13.26 41.42 70.83 52.03 11.86 11.21 11.30 13.03 12.23 13.74 2034 17.99 16 60 1138 13.54 14.40 17.55 11.99 1340 15-06 18.96 17 06 17.07 18.15 18.91 1731 14.97 17.82 16.56 23 61 1839 20.55 16.76 12.02 13.21 20.30 24.95 1330 14.66 21 69 11.48 16.49 15.21 23.20 12.71 1133 13.12 1238 12.90 14.53 1331 1669 14.46 15.91 16.45 1033 11.79 11.93 1331 22.16 13.00 15.09 15.92 1137 11.04 11>40 13.98 14.76 12.74 17.91 16 70 1639 ie.BS 1739 16.50 12.08 1430 15.45 34.63 17.05 16.50 17.13 17.07 15.86 36.26 22.26 17.60 21.20 24.01 2S.Q9 32.23 22.94 21.20 26.82 21.07 20.48 21.46 17.41 22 22 16.06 15.32 18.14 64.81 113.63 61.98 16.24 15.34 15.47 18.02 16.74 18.61 30.71 26.70 27,88 16.10 19.15 20.59 25.62 16.93 19.11 22.09 28.33 26.13 26.60 26.80 26.23 25.69 21.70 26.39 24.37 36.08 27.76 31.16 23.07 1646 18.29 30.67 38.22 18.33 2034 31.98 15.71 23 46 2032 34.63 1740 15.50 1833 17.46 18.06 20.78 18.81 24.58 21.07 23.22 24.17 14.13 15.14 16.33 18.91 33.31 17.76 21.54 22.35 15.84 15.10 15.60 20.13 2132 16.16 26.44 24.44 24.18 28.06 25.38 27.64 17.76 19.84 21.14 42.64 22.48 20.90 22.31 22.30 21.42 45.13 28.50 23.74 28.12 30.62 32.20 40.10 29.23 28.12 34.26 26.93 26.24 27.21 22.70 28.34 21.59 20.67 24.47 77.66 13331 97.63 21 90 20.70 20.B6 24.08 22.59 25.37 37.98 33.61 34.69 21.09 25.06 26.72 32.66 2231 24.86 28.02 35.38 31.76 33.48 33.79 35.25 32.60 27.81 33.19 30.83 44.13 34.66 38.38 29.31 22.20 24 42 37.69 46.66 24.40 27-44 40.37 21.20 30.67 28.08 43.24 23.47 20.91 2438 23.26 23.87 26.95 24 66 31.06 26.89 29.59 30.61 19.07 21.77 22 03 25.00 41.33 24 00 27.99 29.47 21.36 2037 21 04 26.96 27.47 23 63 33.36 31.06 30.64 35.12 32.33 34.48 23.96 26.77 28.51 Table 2.02 Cage NH 3 Mean and Maximum Concentrations for 10 Days 9 51.32 29.08 27.76 28.44 28.53 28.44 54.28 35.57 31.53 36.65 38.60 40.30 48.21 36.26 36.65 42.65 33.54 32.77 33.53 28.98 35.20 28.57 27.45 32.50 8B.S1 146.85 110.26 29.09 27.49 27.71 31.63 30.00 33.69 45.24 40.70 41.70 27.08 32.19 33.89 40.12 28.56 31.63 34.54 42.75 38.96 40.87 41.34 42.58 39.82 34.76 40.49 37.80 51.62 41.68 45.49 36.21 29.48 32.05 45.30 64.63 31 .92 36.44 49.52 26.15 38.97 37.30 52.41 31.17 27.77 31.58 30.40 30.96 34.17 31.69 38,11 33.42 36.70 37.68 25.32 26 91 29.25 32.45 49.60 31.87 35.68 38.13 28.37 27.06 27.95 32.66 34.54 30.06 40.83 36.40 38.30 42.57 40.07 41.79 31.82 35.55 37.87 10 55.30 36.39 36.29 34.67 34.99 37.18 59.00 41.66 41.21 46.42 45.52 47.47 52.37 41.99 46.42 49.71 39.13 36.44 38.34 35.42 40.85 37.11 35.88 42.49 84.85 126.57 103.19 38.02 35.93 36.22 40.61 39.21 44.04 48.23 45.36 45.28 33.42 39,73 40.94 45.60 35.36 36.42 30.52 46.95 44.16 46.64 46.57 46.71 44.72 40.85 45.34 43.05 63.41 46.00 47.92 41.01 38.54 41.10 48.62 55.05 40.69 47.64 56.09 36.80 47.53 46.76 57.95 40.76 36.30 39.88 30.70 38.92 41.25 39.17 43.20 36.85 42.61 42.02 33.10 37.79 38.24 40.61 53.35 41.66 43.14 47.71 37.09 36.37 36.53 38.92 41.07 36.48 46.07 44.18 44.45 47.05 46.34 46.19 41.60 46.47 49.50 Volume II - Section II - Summary of Results of Whole Room Runs Page II - 35 Cas> Nama 1 Basacasa 0.02 Caaa02 0.03 Casa 03 0.03 Casa 04 0.03 Case 05 0.03 Casa 06 0.04 Casa 07 0.03 Casa 08 0.03 Casa 09 0.05 Casa 18 0.02 Casa 11 0.02 Casa 12 0.01 Casa 13 0.02 Casa 14 0.03 Casa 15 0.02 Casa 16 0.02 Casa 17 0.04 Casa 18 0.04 Casa 19 0.03 Casa 20 0.04 Casa 21 0.03 Casa 22 0.03 Casa 23 0.03 Casa 24 0.04 Casa 25 0.03 Casa 26 0.02 Casa 27 0.03 Casa 26 0.06 Casa 29 0.07 Casa 30 0.03 Casa 31 0 06 Case 32 0.06 Casa 33 0.07 Casa 34 0.07 Case 35 0.06 Casa 36 0.07 Casa 37 0.03 Casa 38 0.03 Cass 39 0.04 Casa 40 0.01 Casa 41 0.03 Casa 42 0.03 Casa 43 0.03 Casa 44 0.04 Casa 45 0.03 Casa 46 0.02 Case 47 0 02 Casa 48 0.03 Casa 49 0.03 Casa 50 0.03 Casa 51 0.03 Casa 52 0.03 Casa S3 0.03 Casa 54 0.03 Casa 55 0.03 Casa 56 0 04 Casa 57 0.03 Casa 58 0.04 Casa 59 O.Oi Casa 60 0.03 Casa 61 0.04 Casa 62 0.16 Casa 63 0.11 Casa 64 0.05 Casa 65 0.07 Casa 66 0.17 Casa 67 0.10 Casa 68 0.05 Casa 69 0.03 Casa 70 0 01 Casa 71 0.03 Casa 72 0.02 Casa 73 0.02 Casa 74 0.02 Casa 75 0.02 Casa 76 0.03 Case 77 0.03 Casa 76 0.03 Casa 79 0.04 Casa 80 0.05 Casa 81 0.05 Casa 82 0.06 Casa 63 0.09 Casa 64 O.OS Casa 65 0.07 Casa 86 0.06 Casa 87 0.02 Casa 88 0.02 Casa 89 0.03 Casa 90 0.04 Casa 91 0.03 Casa 92 0.02 Casa 93 0.04 Casa 94 0.06 Case 95 0.04 Case 96 O.OS Case 97 0.06 Casa 98 0.04 Case 99 0.01 CasalOO 0.02 Casa 101 0.15 2 0 04 0.07 0.06 0 06 0.05 0.07 0.06 006 0.09 0.03 O.OS 0.02 0.04 O.OS 005 0.04 O.OS 0.08 0.06 o.oe 0.06 0.06 0.06 0.06 006 0 04 0.06 0.11 0.14 0.06 0.11 0.13 0.14 0.14 0.12 0.14 0.06 0.06 0.07 0.02 0.05 0.06 0.07 0.07 0.05 0.04 0.04 0.07 0.05 0.06 0.07 0.07 0.06 0.06 0.06 0.07 0.06 0.07 0.01 0.06 0.07 0.35 0.22 0.09 0.14 0.32 0.20 0.09 O.OS 0.02 0.05 O.OS 0.04 0.05 0.05 0.06 0.^ 0.06 0.08 0.09 0.09 0.12 0-17 0.15 0.13 0.13 0.05 0.05 0.06 0.07 0.06 0.04 0.09 0.12 0.07 0.10 0.11 0.08 0.03 0.04 0.29 Day by day room breathing 2 ona average NH3 3 4 6 6 7 s'" 0.06 008 0.10 0.15 0.10 0.14 0.10 0.14 0.06 0.11 0.11 0.16 0.09 0.13 0.10 0.14 0.14 0.20 0.05 0.07 0.07 0.11 0.03 0.04 0.06 0.06 0.06 0.12 0.07 0,11 0.06 o.oe 0.12 0.17 0.12 0.18 0.09 0.13 0.12 0.17 0.10 0.14 0.10 0.14 0.10 0.14 0.13 0.16 0.09 0.14 O.OS 0.10 0.10 0.15 0.18 0.25 0.22 0.32 0.10 0.14 0.17 0.24 0.19 0.28 0.22 0.32 021 0.31 0.19 0.27 0.21 0.30 0.09 0.13 0.10 0.14 O.n 016 0.03 0.05 0.08 0.11 0.10 0.14 0-10 0.15 0.11 0.17 0.06 0.12 0.07 0.10 0.06 0.09 0.10 0.15 0.06 0.12 0.09 0.12 0.10 0.15 0.10 0.15 0.09 0.13 0.09 0.13 009 0.13 0.11 0.16 0.09 0.13 0.11 0.15 0.02 0.03 0.09 0.13 0.11 0.15 053 0.77 0.34 0.46 O.IS 0.21 0.21 0.30 0.50 0.72 0.31 045 0.15 0.21 0.08 0.12 0.04 0.05 0.08 0.12 0.07 0.11 0.06 0.08 0.07 0.11 0.07 0.11 0.09 0.12 0.08 0.11 0.09 0.12 0.13 0.18 0.14 0.20 0-14 0.20 0.18 026 0.26 0.38 0.24 0.34 021 0.30 0.19 028 0.07 0.10 0.07 0.11 0.10 0.14 0.11 0.16 0.09 0.13 0.06 0.09 0.13 0.19 0.18 026 0.11 0.16 0.16 0.22 0.17 0.25 0.13 0.19 0.04 0.06 0.06 0.09 0 44 0 .&4 0.11 0.16 021 0.28 0.20 0.26 0.20 027 0.16 0.22 0.23 0.31 0.16 0.25 0.20 0.28 0.28 0.36 0.09 0.13 0.15 021 0.05 007 0.12 0.16 0.16 0.23 0 IS 0.21 0.12 0.16 0.25 0.34 025 0.35 0.19 0.26 0.24 0.33 0-20 0.27 019 0.27 0.20 028 026 0.36 0.19 0.26 0.18 0.26 0.21 0.30 0.36 0.50 0.44 0.62 0.20 0.26 0.34 0.47 0.39 0.6S 0.45 0.63 0.43 0.60 0.39 D.54 0.42 0.59 0.19 026 0.20 0.27 0.22 0.31 0.07 0.09 0.16 022 0.20 0.28 0.21 0.29 0 23 0.32 0.17 0.23 0.14 0.19 013 0.17 0.20 0.28 0.16 0.23 0.17 0.24 0.21 0.29 0-21 0-28 0.18 026 0.19 0.26 0.18 025 022 0.30 0.18 0.25 021 0.30 0.05 0.06 0.18 0.25 022 0.30 1.08 1.50 0.63 0.94 0.29 0.41 0.42 0.59 1.01 1.40 0.64 0.83 029 0.41 0.16 022 O.Oa 0.10 0.16 0.23 0-15 0.21 0.12 0.16 015 0.21 0.15 021 0.17 0.24 0.16 022 0.17 0.24 0.26 0.36 0.29 0.40 0.28 0.39 037 031 033 0.73 0.48 0.67 0.42 D.53 0.39 035 0.14 0.20 0.15 0.21 020 0.27 0.23 031 0.18 0.25 0.13 0.1B 027 038 0.37 031 022 0.30 0.32 0.44 0.35 0.49 0.26 037 0 09 0.12 0.13 0-18 O.SO 1 .25 0.22 0.29 039 0.53 0.38 0.52 0.37 0.50 0.30 0.40 0.43 0.53 0.34 0.46 0.38 0.52 0.53 0.71 0.16 0.24 0.29 0.39 0.10 0.14 0.22 0.30 0.31 0.42 0.29 0.39 0.22 0.30 0.47 0.63 0.47 0.64 0.35 0.48 0.45 0.61 0.37 O.SO 0.37 0-49 0.38 0.51 0.49 0.66 0.36 0.49 0.40 0.49 0.42 0.56 0.68 0.92 0.84 1.14 0.36 0.52 0.64 0.86 0.7S 1.01 0.86 1.16 0.82 1.10 0.73 0.59 0.60 1.08 0.35 0,47 0.37 0.60 0.43 038 0.13 0.17 0.30 0.41 0.38 031 0.39 0.63 0.44 0.60 0.31 0.42 0.26 0.35 0.24 0.32 0.39 0.52 0.31 0.42 0.33 0.44 0.40 0.S4 0.39 0.53 0.35 0.47 0.36 0.49 034 0.46 0.42 0.55 0.34 0.46 0.41 0.55 0.09 0.12 0.34 0.46 0.41 0.56 2.05 2.77 1.29 1.74 0.56 0.75 0.60 1.08 1.91 2.58 1.21 1.63 0.56 0.75 0.31 0.41 0.14 0.19 0.31 0.42 0.29 039 032 0.30 039 0.39 0.29 0.39 033 0.45 0.30 0.41 033 0.45 0.49 0.66 0.54 0-73 034 0.72 0.70 0-64 1.00 1.36 D-Sl 1.23 0-79 1.07 0.75 1.01 037 036 0.29 039 0-37 030 0.43 0.55 0.34 0.46 0.25 0.33 032 0-70 0.70 0.65 0.42 0 35 0.60 D.B1 0.67 030 030 0-68 0.16 032 034 032 1.71 2.30 9 0 39 0.70 0.69 0.67 0.53 0.77 0.61 0.68 0.G4 0.31 0.51 0.16 0.39 0.56 0.52 0.39 0.83 0.67 0.65 0.68 0.83 0.65 0.59 0.72 1.22 1.S1 0.68 I.IS 1.34 1.54 1.46 1.31 1-44 0.63 0.67 0.76 033 0.SS 0.68 0.70 0.79 0-47 0.43 0.69 0.S6 0.59 0.72 0.70 0.53 0.84 0.61 0.74 0.62 0.73 0.16 0.61 0. 74 3.66 2.31 1.00 1.44 3.42 2.16 1. C-0 035 0.26 0.55 032 0.40 031 0.51 039 0.S4 939 D-S7 037 0-95 1.25 1.79 1.64 1.42 1.34 0.48 031 0.67 0.77 •0-61 0.44 ■0.93 1.26 0.73 1.07 1.20 030 0.29 0.43 3.06 10 0.51 0.91 0.90 0.87 0.69 1,01 0.80 0.89 1.23 0.41 0.67 0.24 0.52 0.73 0.67 0.52 1.09 1.11 0.83 1.05 0.87 0.86 0.89 1.1S 0.85 0.62 O.SO 1.59 1.97 0.89 1.50 1.76 2.01 1.91 1,72 1.88 0.82 0.88 1.00 0.30 0.71 0-89 031 1.04 0.74 0.61 0.56 0.91 0.94 031 0.82 0.84 0.80 0.S7 0.81 0.85 0.21 0.8 D 0.97 4.81 3.02 1.31 1.88 4.47 2.63 1.31 0.72 0.68 032 0.67 0.67 0.78 0.71 0.78 1.14 1.27 1.25 1.63 2.34 2.14 1.86 1.76 0.87 0.87 1.01 0.80 1.21 1.64 0.58 1.40 1.56 1.17 036 036 4.00 Day by day room breathing zone maximum NH3 1 2 3 4 5 6 7 6 " 0.07 0.09 0.09 0.09 0 06 0.08 0.19 0.12 0.14 0.08 0.12 0.12 0.16 0.11 0.16 0.13 0.09 0.09 0.12 0.09 0.1C 0.11 0 09 0.22 0.12 0.06 0.12 0.16 0.14 0.13 0.16 0.18 0.22 0.17 0.15 0.16 0 09 0.10 0.13 0.04 0.07 0.10 0.09 0.12 0.13 0.10 0.09 0.16 0.13 0.10 0.12 0.10 0.09 0.15 0.13 0.08 0.15 0.13 0.05 0.09 0.16 0.47 0.34 0.17 0.17 0.43 0.32 0.14 0.14 0.06 0.16 0.13 0.08 0.13 0.13 0.12 0.10 0.11 0.10 0.11 0.14 0.17 031 030 0.23 0.19 0.07 0.04 0.11 0.15 0.10 0.06 0.19 0.17 0.15 0.15 0.15 0.17 0.06 0.10 0.41 0.14 0.18 0.17 0.17 0.16 0.16 0.36 0.23 0.28 0.16 0.23 033 0.28 0.22 0.31 0.25 0.18 0.18 0.22 0.18 0.19 0.22 0.18 0.43 0.24 0.15 0.24 0.31 0.27 0.25 0.32 0.34 0.44 0.32 039 0.31 0.18 030 035 0.09 0.14 0.19 0.17 0.24 0.25 0.20 0.18 0.31 0.25 030 033 0.37 0.17 0.29 0.24 0.16 0.28 036 0.09 0.18 0.31 0.91 0.66 0.33 0.32 0.83 0.63 0.26 036 0.12 031 036 0.15 035 035 0.24 0.19 031 0.20 031 038 0.34 0.41 0.38 0.4S 0.38 0.14 0.09 032 0.28 0.19 0.15 0-37 0.33 038 0.28 030 D.34 0.12 D.19 O.BD 0.21 0.28 0.27 036 0.24 0.25 0.5B 0.35 0.43 0.25 0.35 0.36 0.44 0.34 0.48 0.39 0.26 0.28 0.35 0.27 0.29 0.34 0.28 0.67 0.36 0.23 037 0.48 0.42 0.39 0.49 0.53 0.67 0.50 0.45 0.47 0.28 0.31 0.39 0.13 0.22 0.30 036 0.37 0.38 0.31 0.27 0.47 0.38 0.31 0.36 037 036 0.44 0.38 0.25 0-44 0.40 0.14 0.27 0.48 1.40 1.01 031 030 1.28 0.37 0.41 0.41 0.19 0.48 0.40 0.23 0.39 039 0.37 030 0.33 030 032 0-43 032 0.53 038 0.39 038 0.21 0.13 0.34 0.44 0.29 0.24 036 031 0.44 0.44 0.46 032 0.19 0-30 1.24 0.31 0.41 0.38 0.36 0.35 0.35 0.83 0.51 0.62 0.36 0.50 0.52 0.63 0.49 0.89 0.S7 0.41 0.41 0.50 0.39 0.42 0.49 0.41 0.86 0.S2 0.45 0.57 0.69 0.60 0.57 0.71 0.76 0.97 0.71 0.S5 0.58 0.40 0.44 038 0.19 0.31 0.43 0.37 0-53 0.55 0.45 0.40 0.58 035 0.44 032 0.32 038 0.54 034 0.35 0.53 037 0.20 0.39 0.70 2.02 1.46 0.73 0.72 1.84 1.40 039 039 037 0.59 037 0.33 036 036 033 0.43 0.48 0.44 0.47 0-S3 0-75 0.91 C. 35 1.00 0-W 0.30 0.19 0.49 0-S3 0.41 034 0.51 0.74 D. S3 O.S3 0..S6 0.73 0.2B 0.43 1.78 0.43 0.57 0.54 0.54 0.49 O.SO 1.17 0.71 0.80 O.SO 0.70 0.73 0.89 0.70 0.97 0.30 0.57 0.57 0.70 0.55 0.59 0.59 0.57 1.35 0.74 0.76 0.83 0.97 0.85 O.SO 1.00 1.07 137 1.01 0.92 0.96 0.56 0.62 0.79 0.27 0.44 050 0.52 0.75 0.78 0.83 036 0.9S 0.78 0.62 0.72 1.15 0.53 0.90 0.76 030 0 88 0.30 039 0.5S 0.9B 2.84 2.06 1.02 1.02 2.59 1.97 G.83 0.82 0.39 0.97 O.SO 0.46 0.73 C.78 0.75 0.60 037 0-32 0.35 0-58 1.05 1.27 1.20 1.41 1.18 0.42 0.27 0 59 0-5B D3B 0.48 1.14 1.04 C'.SS 039 0.93 1.06 0-39 0-51 2.S1 0.50 0.79 0.75 0.74 0.67 0.69 1.63 0.99 1.21 0.69 0.9S 1.01 1.23 0.96 1.34 1.10 0.79 O.SO 0.97 0.76 0.S2 0.95 0.79 1.87 1.02 1.13 1.18 1.35 1.17 1.10 1 38 1.48 1.00 1.39 1.26 1.33 0.78 0.86 1.09 0.37 0.51 0.83 0.72 1.04 1.0B 0.66 0.77 1.32 1.08 0.87 1.00 1.59 0.74 1.24 1.06 0.59 1.22 1.11 0.40 0.76 1.36 3.94 2.85 1.42 1.41 3.59 2.73 1.15 1.14 034 1.34 1.11 0-B4 1.08 1.08 1.04 0.54 033 0.56 031 1.22 1.46 1.77 1,67 1.95 1.64 039 0-38 0.95 1.22 D.3D 0 55 1.50 1.44 1.23 1.23 1.29 1.46 034 0-54 3.48 0.83 1.08 1.02 1.02 0.92 0.94 2.23 1.35 1.66 0.95 1,34 1.39 1.69 1.32 1.63 1.51 1.08 1.09 1.33 1.05 1,12 1.30 1.06 2.56 1.40 1.71 1.65 1.84 1.60 1.61 1.69 2.02 2.60 1.91 1.75 1.62 1.07 1.18 1.50 031 0.84 1.14 0-98 1.42 1.48 1.20 1.06 1.81 1.48 1.19 1.37 2.18 1.01 1.70 1.45 0.94 1.68 1.52 0-S4 1.05 1.86 5.39 3.90 1.94 1.93 4.91 3.74 1.57 1.56 0.73 1.83 1.52 0.37 1.46 1.48 1.42 1.14 1.27 1.17 1.24 1.67 2.00 2.42 2.28 2.67 2.24 0.31 0.S1 1.30 1.68 1.10 0-90 2.17 1.97 1.69 1.68 1.76 2.00 0.74 1.16 4.76 1.11 1.46 1.38 1.37 1.24 1.27 3.00 1.82 2.24 1.28 1.80 1.87 2.27 1.78 2.48 2.04 1.46 1.47 1.60 1.41 1.61 1.75 1.46 3.46 1.88 2,12 2.18 2.48 2.16 2.04 2.55 2.73 3.50 2.57 2.36 2.46 1.44 1.59 2.02 0.59 1.13 1.53 1.32 1.92 1.99 1,62 1.43 2.44 2.00 1.60 1.85 2.94 1.36 2.30 1.95 1.27 2.26 2.05 0.73 1.41 2.61 7.27 5.26 2.62 2.60 6.63 5.05 2.12 2,11 0.99 2.47 2.05 1,18 2.00 2.00 1.91 1.54 1.71 1.58 1.68 2.25 2.69 3.26 3.07 3.60 3.03 1.09 0-99 1.75 2.26 1.48 1.22 2.92 2.66 2.27 2.27 2.38 2.70 HjOD 1.56 6.42 9 1.40 1.94 1.83 1.83 1.65 1.69 3.99 2.42 2.98 1.70 2.30 2.48 3.02 2.36 3.29 2.70 1.94 1.95 2.39 1.87 2.00 2.33 1.94 4.59 2.60 2.52 2.81 3.30 2.68 2.71 3.39 3.63 4.65 3.42 3.13 3.26 1,92 2.12 2.68 0.92 1.50 2.03 1.76 2.55 2.64 2.15 1.89 3.25 2.65 2.12 2.46 3.90 1,81 3.05 2.59 1.69 3.00 2.73 0.97 1.87 3.33 9.65 6.99 3.46 3.45 8.81 6.70 2.61 2.60 1.31 3.28 2.73 1.57 2.66 2.66 2.54 2.05 2 28 2.10 2.23 2.99 3.58 4.33 4.08 4.78 4-02 1.44 0.92 3.00 1.97 1.62 3.86 3,63 3.02 3.01 3.16 3.59 1.33 2.07 8.53 10 1.93 2.53 2.39 2.39 2.16 2.21 5.21 3.16 3.89 2.22 3.13 3.26 3.95 3.09 4.30 3.53 2.54 2.55 3.12 2.45 2.62 3.04 2 54 6.00 3.27 2.66 3.61 4.31 3.76 3.54 4.43 4.74 6.08 4.47 4.09 4.27 2.51 2.77 3.61 1.20 1.97 2.66 2.30 3.33 3.46 2.81 2.48 4.24 3.47 2.78 3.22 5.10 2.36 3.98 3.39 2.21 3.92 3.57 1.27 2.45 4.36 12.61 9.13 4.55 4.51 11.51 8.76 3.68 3.66 1.72 4.29 3.56 2.05 3.48 3.46 3.32 2.68 2.98 2.74 2.91 3.91 4.67 5.67 5.34 6.25 5.26 1.89 1.21 3.04 3.92 2.67 2.12 5.06 4.62 3.95 3.94 4.13 4.69 1.73 2.71 11.15 Table 2.03 Room NH 3 Concentrations for 10 Days with Increased Cage Temperature Volume II -36 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Cosa Name 1 Basecaso 1.12 Case 02 0.89 Case 03 0.82 Case 04 0.89 Case 05 0.91 Case 06 0.86 Case 07 1.21 Case 08 0.97 Case 09 0.90 Case 10 1.10 Case 11 1.10 Cose 12 1.15 Case 13 1.30 Case 14 1.16 Case 15 1.10 Case 16 1.16 Case 17 0.94 Cose 16 0.93 Cose 19 0.90 Case 20 0 66 Case 21 0.94 Cose 22 0.67 Case 23 0.66 Case 24 0.98 Case 25 1.56 Case 26 2.12 Case 27 1.66 Case 28 0.91 Case 29 0 92 Case 30 0.87 Case 31 1.01 Case 32 0.91 Case 33 1.03 Case 34 0.99 Case 35 0.92 Case 36 0.98 Case 37 0.89 Case 36 0.92 Case 39 0.96 Case 40 1.03 Case 41 0.86 Cose 42 0.90 Cose 43 0.97 Case44 1.10 Case 45 1.00 Case 46 1.01 Cose 47 1.02 Case 46 109 Case 49 1.05 Case 60 0.96 Case 51 1.06 Case 52 0.87 Case 53 1.16 Case 64 0.97 Case 55 1.06 Case 66 0 98 Cose 67 0.96 Cose 56 1.03 Case 59 1.12 Cose 60 1 .22 Cose 61 1.06 Cose 62 1.21 Case 63 1.06 Case 64 0.94 Case 65 1.04 Cose 66 1.21 Case 67 1.11 Case 66 0.96 Cose 69 0.91 Case 70 1.02 Case 71 1.02 Cose 72 0.93 Cose 73 1.02 Case 74 0.98 Case 75 0.96 Cose 76 0.97 Cose 77 0.96 Cose 70 1.00 Case 79 0.86 Case 80 0.89 Cose 61 0.91 Cose 02 0.87 Case 83 0.91 Case 84 0.96 Cose 65 1.00 Cose 86 1.00 Case 87 0.72 Case 68 0.81 Case 89 0.85 Case 90 0.87 Casa 91 0.69 Cose 92 0.66 Case 93 0.95 Case 94 0.91 Cose 95 0.05 Case 96 1.02 Case 97 0.60 Case 96 1.02 Case 99 103 Cose 100 0.90 Case 101 1.26 2 2.18 1.72 1.59 1.73 1.76 1.67 2.35 1.89 1.75 2.14 2.14 2.23 2.52 2.24 2.14 2.24 1.84 1.81 1.76 1.66 1.83 1.69 1.67 1.91 3.04 4 02 3.21 1.77 1.79 1.69 1.96 1.77 2.00 1.92 1.70 1.90 1.74 1.60 1.87 2.00 1.71 1.76 1.86 2.14 1.95 1.97 1.98 2.12 2.05 1.86 2.07 1.69 2.24 1.89 2.06 1.91 1.88 2.01 2.19 2.36 2.07 2.35 2.11 1.63 2.03 2.35 2.17 1.66 1.77 1.96 1.99 1.81 1.99 1.91 1.90 1.94 1.67 1.73 1.76 1.69 1.78 1.66 1.95 1.94 1.41 1.67 1.73 1.66 1.84 1,70 1.84 1.98 1.75 1.98 2.01 1.75 2,43 Doy fay day cage average NH3 3 4 5 6 7 8 9 10 3.37 2.66 2.45 2.67 2.72 2.58 3.62 2.92 2.69 3.31 3.30 3.45 3.69 3.46 3.31 3.45 2.63 2,79 2.69 2.56 2.62 2 60 2.56 2.94 4.69 6.20 4.95 2.73 2.76 2.60 3.02 2.73 3.06 2.96 2.76 2.93 2 68 2.77 2.66 3.06 264 2.70 2.90 3.31 3.01 3.03 3.06 3.26 3.16 2.67 3.19 2.60 3 45 2 92 3.16 2.94 2.69 3.10 3.37 3.67 3.19 3.63 3.26 2.62 3.12 3.62 3.34 2.07 2.73 3.05 3.07 2.70 3.07 2 94 2.07 2 90 2.03 2.99 2.57 2.66 2.72 2.60 2.74 2.89 3.01 2.90 2.17 2 42 2.65 2.61 2 67 2.55 2.64 2.74 2.64 3.05 2.69 3.05 3.10 2.70 3.74 4.85 3.83 3.53 3.64 3.02 3.72 5.22 4.21 3.66 4,77 4.76 4.07 5.60 4 98 4.77 4.98 4.08 4.03 3.88 3.72 4.07 3.75 3.71 4.24 6.77 11.92 7.53 3.93 3.97 3.76 4.36 3.93 4 44 4 26 3.06 4.22 3.86 3.99 4.16 4 44 3.60 3.69 4.17 4.76 4 34 4 37 4.41 4.72 4 55 4.14 4.60 3.75 4 97 4.20 4 50 4 24 4.17 4.47 4.86 5.29 4.69 6.23 4.68 4.07 4.S0 521 4.62 4.13 3.94 4.40 4.43 4.03 4 42 4 24 4.14 4.18 4 23 4 32 3.70 3.64 3.92 3.76 3 96 4,17 4 34 4.31 3.13 3.49 3.66 3.76 3.64 3.67 4.10 3.95 4.10 4.40 3 88 4 40 4 46 3.89 5.40 6.63 5.39 4.96 6.40 5.51 5.22 7.34 5.92 5.46 6.70 6.70 6.96 7,68 7.01 6.70 7.00 5.74 6.67 5.46 5.24 5.72 6.27 6.22 5.96 9.51 20.30 11.08 5.53 5.58 5.27 6.13 6.53 6.24 6.00 5.67 5 94 6-43 6.62 5.65 6.25 5 35 5.47 5.07 6 70 6.10 6.14 6.20 664 6.41 5.82 6.47 5.27 7.00 S.91 6.45 5.96 6 67 6.29 6.64 7.45 6.46 7,36 6.S6 5.72 6.33 7.33 6,78 6.81 6.64 6.16 6 22 5.66 6.22 5.96 5.62 6.87 5.95 6.07 6.21 5.40 5.51 5.27 S.5S 5.86 6.11 6.06 4 40 4.90 5.17 6.20 5.41 5.16 5.76 6.66 5.76 6.19 5,40 6.16 6.26 5 47 7.59 9 47 7.47 6.86 7.49 7.64 7.24 10,18 6.21 7.57 9.29 9.20 9.66 10.93 9.72 0.29 0.71 7.98 7.86 7.57 7.26 7.93 7.30 7.24 6.26 13.10 30.25 15.62 7.67 7.74 7.31 ,6.60 7.M 8.66 8.31 7.72 6.24 7.52 7.79 0.11 6.66 7.41 7.60 8.14 0.29 6.46 6.62 6.60 9.21 8.66 6.07 6.97 7.30 9.70 8.20 8.04 8.27 6.14 0.73 9.46 10.32 6.96 10.20 9.13 7.93 6.76 10.17 9.40 6.06 7.60 6.58 6.63 7,66 8.62 8.27 6.07 8.15 8.26 8.42 7.22 7.49 7.64 7.31 7.70 8.13 8.47 8.41 6.10 6.60 7.17 7.34 7.60 7.10 7.99 7.71 7.90 8.58 7.57 8.57 8,70 7,69 10.53 12.96 10.22 9.41 10.26 10.46 9.92 13.94 11.23 10.38 12.72 12.71 13.25 14 95 13.30 12.72 13.29 10.69 10,75 10.36 9.94 10 65 10.00 6.91 11.30 18.05 45.71 21 95 10.50 10.59 10,01 11.63 10.46 11.65 11.36 10.67 11.27 10.30 10.66 11,09 11.66 10.16 10 37 11.14 12.71 11.56 11.66 11.77 12.60 12.16 11.05 12.27 10.00 13.26 11.22 12.24 11.32 11.13 11.94 12.07 14.13 12.26 13.96 12.40 10.65 12.02 13.01 12.67 11.03 10.61 11.74 11.01 10.74 11.60 11.31 11.04 11,16 11.29 11,52 9.66 10.24 10.45 10.00 10.64 11,12 11.60 11.61 6.35 9.30 9.62 10.04 10,26 9.79 10.93 10.65 10.03 11.74 10.35 11.73 11.01 10.38 14 40 17.40 13.79 12.70 13.64 14.11 13.37 18.80 15.15 13.97 17.16 17.15 17.86 20.17 17.94 17.16 17.92 14.69 14.50 13.07 13.41 14.64 13 48 13.37 15.25 24.35 56.46 28.91 14.16 14.26 13.50 16.69 14.16 16.98 15.35 14.26 15.20 13.69 14.38 14.96 16.00 13.69 13.09 15.02 17.15 15.62 15,73 15.68 17.00 16.40 14.90 16.56 13.46 17.91 15.14 16.51 15.27 15.02 16.11 17.50 19.06 16.54 18.63 16.65 14 64 16.21 18.77 17.36 14.60 14.17 15.63 16.93 14.40 15.92 15.26 14.69 16.04 15.23 15.54 13.33 13.82 14.10 13.50 14.22 15.00 15.63 15.62 11.26 12.65 13.26 13.65 13.84 13.21 14.75 14.23 14.75 16.84 13.97 16.83 16.07 14.01 19.43 23 21 18.31 16.87 18 38 18.74 17.76 24.97 20.12 18.55 22.78 22 78 23.75 26.79 23 62 22.70 23.80 19.52 19.26 18.56 17.81 19.44 17.91 17.76 20.25 32.35 67.24 37.36 18.81 18.97 17.03 20.84 10,79 21.23 20 39 18.03 20.19 18.46 10.10 19.88 21.24 16.18 16.58 19.95 22.78 20.74 20.60 21 09 22.67 21.78 19.70 21.99 17.01 23.70 20.10 21.03 20.26 10.95 21.40 23.24 26.32 21.96 26.01 22.38 19 44 21.53 24.92 23.05 19.77 16.82 21.03 21.10 10.25 21.14 20.27 10.70 19.97 20.23 20.64 17.71 16.35 10.72 17.92 18 88 19.03 20.76 20 62 14 96 16.67 17.50 17.90 18.36 17.54 19.69 18 00 19.69 21.04 16.65 21 02 21.34 18.61 25.81 30.34 23.94 22.05 24.02 24.50 23.22 32.63 26.30 24 25 29.78 29.77 31.04 35.02 31.14 29.78 31.11 25.61 26.16 24.26 23.28 26.42 23.41 23.22 26.47 42.28 71.62 46.66 24.60 24.80 23 44 27.26 24.57 27.75 26 65 24.76 26.40 24.11 24.96 26.68 27.77 23.76 24.20 26.08 29.77 27.11 27.31 27.56 26.61 26.47 25,87 28.75 23.41 31.09 26.28 28.67 26.61 26 06 27.97 30.39 33.09 28,71 32.60 29.25 25.42 26.15 32.se 30.13 26.64 24.61 27.49 27.66 25.16 27.63 26.49 25.85 26.11 26 44 26.08 23.15 23.99 24.47 23.43 24.68 26.05 27.14 26.95 19.55 21.78 23.00 23-52 24.02 22.93 25.61 24.70 26.61 27,61 24.25 27.48 27.89 24.32 33.74 1 2 3 1.63 3.17 4.89 1.26 2.51 3.87 1.34 2.61 4.03 1.19 2.32 3.58 1.21 2.36 3,64 1.38 2.68 4.13 1.76 3,43 6,28 1.37 2.67 4.12 1.52 2.67 4.57 1.67 3,24 5.00 1.51 2,04 4.53 1.57 3.06 4 72 1.56 3.04 4.66 1.36 2.65 4.09 1.67 3.24 5.00 1.63 3.16 4.68 1.26 2.49 3 64 1.27 2.47 3.80 1.22 2.36 3.67 1.22 2.38 3,67 1.33 2.69 3.99 1.36 2.66 4.09 1.33 2.56 3.66 1.57 3.06 4 72 2.05 3.96 6.15 2.58 4.90 7.65 2.36 4.61 7.11 1.41 2.74 4.22 1.33 2.59 3.96 1.34 2.61 4.02 1.48 2.07 4.43 1.45 2.62 4.35 1.63 3.17 4.89 1.40 2.72 4,20 1.40 2.72 4.20 1.35 2.63 4.05 1.17 2.27 3.60 1.39 2.70 4.16 1.40 2.72 4.10 1.45 2.81 4.34 1,24 2.41 3.71 1.32 2.57 3.96 1.26 2.46 3.79 1.42 2.77 4.26 1.36 2.71 4.10 1.43 2.78 4,28 1.46 2.84 4.38 1.41 2.75 4.24 1.40 2.72 4.19 1.35 2.63 4.05 1.41 2.74 4.23 1.36 2.66 4.09 1.47 2.86 4.41 1.36 2.71 4.18 1.36 2.66 4.00 1.34 2.60 4.01 1.43 2.77 4.26 1.49 2.00 4.48 1.42 2.77 4.27 1.52 2.96 4.67 1.47 2.86 4.41 1.76 3.43 5.20 1.77 3.44 5.31 1.36 2.65 4.09 1.64 3.19 4.91 1.80 3.61 S.41 1.77 3.45 5.31 1.61 2.63 4.52 1.34 2.61 4.03 1.43 2,77 4.28 1.40 2.72 4 10 1.36 2.69 4 16 1.41 2,73 4,22 1.37 2.66 4.11 1.36 2.65 4 09 1.27 2.46 3.80 1.38 2.68 4.14 1.37 2-66 4.10 1.22 2,38 3.67 1.40 2.72 4.19 1.41 2.75 4.24 1.45 2. 63 4.36 1.67 3.06 4.72 1.54 3.00 4.62 1.48 2.67 4.43 1.69 3.29 5.07 1.37 2.67 4.12 1.31 2.55 3.03 1.35 2,63 4,05 1.31 2.54 3.62 1.37 2.60 4.12 1.26 2.43 3.76 1.45 2.81 4.34 1-42 2.77 4.27 1.45 2.81 4.34 1.44 2.60 4.31 1.50 2.62 4.60 1.41 2.74 4.23 1.54 3.00 4 62 1.72 3,35 5.16 1.63 3.66 6.50 Day by day cage maximum NH3 4 5 6 7 6 9 10* 7.05 5.57 5,81 5.16 5.25 6.95 7.61 5.93 6.59 7.20 6.53 6.60 6.75 5.69 7.20 7.03 5.54 5.46 5.29 5.29 5.75 5.60 5.74 6.80 6.86 14.51 10.62 6.08 5.75 5.79 6.36 6,27 7.05 6.05 e.os 5-63 6.05 6.00 6.04 6.25 5.35 5.70 5.46 6.15 6.03 6.18 6.31 6.11 6.03 6.63 6.09 5.90 6 36 6.02 6.00 5.78 6.17 6.45 6.16 6.59 6.35 7.62 7.65 5.89 7.08 7.80 7.66 6.52 5.81 6.16 6.03 6.99 6.08 6.92 5.90 5.46 6.97 6.00 5.30 6.05 6.12 6.26 6.60 8.67 6.39 7.31 5.63 5.66 5.65 6.66 5.96 6.41 6.25 6.15 6.26 6 21 6.49 6.10 6.66 7.43 7.92 9.91 7.04 6.16 7.26 7.38 8.37 10.71 8.34 9.27 10.13 9.18 9.56 9.46 6.26 10.13 6.69 7.79 7.70 7.44 7.44 e.oe 8.30 8.07 9.56 12.46 24.70 15.90 6.66 8.06 8.15 8.96 6.82 9.61 6.50 8.51 6.20 7.10 8.43 6.49 6.79 7.53 6.02 7.66 8 64 6.48 8.66 8 67 6.59 6.49 8.20 0,57 8.30 8.94 8 47 6.30 8.13 8.67 9.07 6.66 9.27 6.03 10.72 10.76 8 28 9.96 10,97 10.77 9.17 8.17 6.67 8.49 8.42 6.54 8.33 8.29 7.70 8.36 8 30 7.45 8.50 8.60 6.64 0 56 9.37 6.66 10.28 6.34 7,06 8.22 7.64 6.36 7.60 0.79 8.65 8-79 8.74 9 13 8.56 9.36 10.46 11.14 13.74 10.87 11.32 10.07 10.23 11.60 14.85 11.57 12.86 14.04 12.73 13.26 13.16 11.49 14.04 13.71 10.81 10.68 10.32 10.32 11.21 11.51 11.16 13.26 17.28 36.81 22.45 11.86 11.21 11.30 12.45 12.23 13.74 11.76 11.80 11.38 6.84 11.69 11.77 12.19 10.44 11.12 10.65 11.98 11.76 12.04 12.30 11.91 11, n 11.38 11.86 11.51 12.36 11.74 11.61 11.27 12.02 12.58 12.01 12.85 12.39 14.86 14.92 11 48 13.81 16.21 14.63 12.71 11.33 12.02 11.77 11,60 11.85 11.55 11.60 10.66 11.63 11.61 10.33 11.79 11.93 12.26 13.26 13.00 12.46 14,25 11,57 11.04 11.40 11.01 11.69 10.65 12.19 11.90 12.16 12.12 12.65 11.89 12.96 14.60 15 45 16.81 14.87 15.50 13.78 14.00 15.88 20.32 15.63 17.60 19.22 17.43 16.15 18.01 15,72 19.22 18.76 14.79 14.62 14.13 14.12 15.34 15.76 15.32 16.14 23.64 55.62 31.65 16.24 15.34 15.47 17.04 16.74 18.81 16.14 16.16 15.57 13.47 16.00 16.11 16.68 14.26 15.22 14.58 16.40 16.09 10.48 16.64 16.29 16.10 16.57 16.26 15.75 16.96 16,07 15.75 15.43 16.46 17.22 16.44 17.58 16.95 20.34 20.42 15.71 18.60 20.82 20.43 17.40 16.50 10.45 16.10 16.98 16 22 15.80 16.74 14 62 15.02 15.76 14.13 16.14 16.33 16.77 18.14 17.79 17.05 19.50 16.84 15.10 15.60 16,07 16-87 14.43 16.69 16.41 16.69 16.58 17,32 16.26 17.76 19 64 21.14 25.37 20.06 20.90 16.59 16.69 21.42 27.41 21.36 23.74 25.92 23.51 24.48 24.30 21.21 25.92 25.31 19.05 19.72 19.06 19.05 20.69 21.24 20.67 24.47 31.89 68.72 41.67 21.90 20.70 20.86 22.90 22.59 25.37 21.77 21.79 21.00 18.17 21.58 21.73 22.50 19.28 20.62 19.67 22.12 21.71 22.23 22.72 21.66 21.72 21.00 21.94 21-24 22.86 21.66 21.24 20.81 22.20 23.23 22.16 23.72 22.87 27.44 27.55 21.20 25.49 28.06 27.67 23.47 20.61 22.19 21.72 21.56 21.87 21.32 21.23 19.72 21.47 21.25 19.07 21.77 22.03 22.63 24.48 24.00 23.00 26.31 21.36 20.37 21.04 20.33 21.40 19.47 22.61 22.14 22.51 22.37 23.36 21.96 23.96 26.77 28.51 33.70 26.65 27.76 24 69 26.09 26.44 36.40 28,37 31,63 34.43 31.22 32.52 32,27 26.16 34.43 33.62 26.49 26.19 25.31 26.30 27.46 28.21 27.46 32.50 42.36 81.62 53.72 26.09 27.49 27.71 30.63 30.00 33.69 26.61 26.94 27.89 24.13 28.67 28.87 29.86 25.60 27,26 26.12 26.36 28.63 29.63 30.17 29.19 28.85 27.69 29.14 28.21 30.39 20.79 26.21 27.64 29.46 30.85 29.45 31.60 30.37 36.44 36.50 28.15 33.66 37.30 36.61 31.17 27.77 29.48 28 85 28.63 29.05 28.32 26.20 26.19 26.52 26.23 25.32 28.91 29.25 30.05 32.51 31.87 30.54 34.94 28.37 27.00 27.95 27.00 28.43 25.66 29.90 29.40 29.90 29.71 31,03 29.17 31.82 35.55 37.67 44.05 34.63 36.29 32.27 32.80 37.18 47.59 37.00 41.21 45.00 40.81 42.50 42.19 36.82 45.00 43.95 34.63 34.24 33.08 33.07 35.92 36.88 35.88 42.49 55.37 87.03 67.08 36.02 35.93 36.22 39.90 39.21 44.04 37.60 37.63 36.46 31.54 37.47 37.73 39.06 33.47 35.63 34.16 38.41 37.69 38.60 39.44 38.16 37.71 36.46 36.09 36.66 39.72 37.64 36.68 36.14 36 54 40.33 38.50 41.10 39.70 47.64 47.63 36.80 44.26 48.76 47.06 40.76 36.30 36.53 37.71 37.43 37.98 37.01 36.86 34.23 37.28 36.00 33.10 37.79 36.24 39 28 42.49 41.66 39.93 45.67 37.09 35.37 36.53 36.30 37.16 33.60 39.08 36.43 39.06 36.64 40.56 38.12 41.^ 48.47 49.50 Table 2.04 Cage NH 3 Concentrations for 10 Days with Increased Cage Temperature Volume II - Section III - Rankings of Results of Whole Room Runs Page II • 37 3. RANKINGS OF RESULTS OF WHOLE ROOM RUNS 3.1 Introduction The following tables present rankings of the results of the CFD room simulations based on Room (scientists’ breathing zone) and cages mean values for CO 2 and NH3 (day 4) concentrations, temperature, and relative humidity. The tables start with the case with the lowest value and list the rest in ascending order. The case number should be used to identify all details of the case. The supply diffuser type, exhaust location, supply flow rate, and supply discharge temperature are given to provide a quick overview of the case. Discussion of these tables can be found in volurpe I, section 3.3. VoSume II -38 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 3.01 Ranking of Cases with respect to Room Temperature Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room Temp CC) Room Temp CF) Case 66 Lx)w Ind Low 5 6.6 43.9 18.6 65.6 Case 62 Low Ind Low 5 6.6 43.9 19.1 66.5 Case 53 Radial Ceiling 15 18.8 65.8 19.8 67.6 Case 55 Low Ind Ceiling 15 18.8 65.8 19.8 67.6 Case 54 Slot Ceiling 15 18.8 65.8 19.8 67.6 Case 36 Low Ind Ceiling 15 17.5 63.5 19.9 67.8 Case 35 Slot Ceiling 15 17.5 63.5 20.0 67.9 Case 34 Radial Ceiling 15 17.5 63.5 20.0 67.9 Case 07 Low Ind Ceiling 15 18.8 65.8 20.0 67.9 Case 98 Low ind Ceiling 15 17.5 63.5 20.0 68.0 Case 96 Radial Ceiling 15 17.5 63.5 20.0 68.0 Case 97 Slot Ceiling 15 17.5 63.5 20.0 68.0 Case 16 Radial Ceiling 15 18.8 65.8 20.1 68.1 Case 46 Radial Ceiling 15 18.8 65.8 20.2 68.3 Case 67 Low Ind Low 10 14.8 58.6 20.2 68.3 Case 19 Radial Ceiling 15 18.8 65.8 20.2 68.4 Case 18 Low Ind Ceiling 15 18.8 65.8 20.2 68.4 Case 17 Slot Ceiling 15 18.8 65.8 20.2 68.4 Case 94 Slot Ceiling 15 17.5 63.5 20.2 68.4 Case 95 Low Ind Ceiling 15 17.5 63.5 20.2 68.4 Case 43 Low Ind (rot 90°) Ceiling 15 18.8 65.8 20.2 68.4 Case 47 Radial Ceiling 15 18.8 65.8 20.3 68.5 Case 93 Radial Ceiling 15 17.5 63.5 20.3 68.5 Case 45 Radial Ceiling 15 18.8 65.8 20.3 68.5 Case 60 Low Ind High 15 18.8 65.8 20.3 68.5 Basecase Radial Ceiling 15 18.8 65.8 20.3 68.5 Case 25 Radial Ceiling 15 18.8 65.8 20.3 68.5 Case 27 Low Ind Ceiling 15 18.8 65.8 20.3 68.6 Case 56 Radial High 15 18.8 65.8 20.3 68.6 Case 59 Slot Low 15 18.8 65.8 20.3 68.6 Case 63 Low Ind Low 10 14.8 58.6 20.3 68.6 Case 90 Radial Ceiling 15 18.8 65.8 20.4 68.7 Case 77 Slot Ceiling 15 18.8 65.8 20.4 68.7 Case 76 Radial Ceiling 15 18.8 65.8 20.4 68.7 Case 21 Low Ind Ceiling 15 18.8 65.8 20.4 68.7 Case 92 Low Ind Ceiling 15 18.8 65.8 20.4 68.7 Case 26 Slot Ceiling 15 18.8 65.8 20.4 68.7 Case 78 Low Ind Ceiling 15 18.8 65.8 20.4 68.7 Case 04 Slot Ceiling 15 18.8 65.8 20.4 68.8 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 20.4 68.8 Case 91 Slot Ceiling 15 18.8 65.8 20.5 68.8 Case 50 Radial Ceiling 15 18.8 65.8 20.5 68.8 Case 52 Radial Ceiling 15 18.8 65.8 20.5 68.8 Case 08 Low Ind High 15 18.8 65.8 20.5 68.9 Case 42 Low Ind Ceiling x4 15 18.8 65.8 20.5 68.9 Case 51 Radial Ceiling 15 18.8 65.8 20.5 69.0 Case 05 Slot High 15 18.8 65.8 20.5 69.0 Case 41 Slot Ceiling x4 15 18.8 65.8 20.5 69.0 Case 40 Radial Ceiling x4 15 18.8 65.8 20.5 69.0 Case 49 Radial Ceiling 15 18.8 65.8 20.5 69.0 Case 20 Slot Ceiling 15 18.8 65.8 20.6 69.0 Case 48 Radial Ceiling 15 18.8 65.8 20.6 69.1 Case 02 Radial High 15 18.8 65.8 20.7 69.2 1 Case 37 Radial Ceiling/Low 50/50 15 18.8 65.8 20.7 69.3 Volume II - Section III - Rankings of Results of Whole Room Runs Paee II - 39 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room Temp (°C) Room Temp (°F) Case 85 Radial Low 15 17.5 63.5 20.7 69.3 Case 38 Slot Ceiling/Low 50/50 15 18,8 65.8 20.8 69.4 Case 39 Lx)w Ind Ceiling/Low 50/50 15 18.8 65.8 20.8 69.4 Case 65 Low Ind Low 15 17.5 63.5 20.8 69.5 Case 13 Slot Low 15 20.7 69.3 20.8 69.5 Case 10 Radial Ceiling 15 20.7 69.3 20.9 69.7 Case 72 Radial High x4/ Low x2 15 18.8 65.8 21,1 69.9 Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 21.1 70.0 Case 75 Radial Ceiling/Low 50/50 15 18.8 65,8 21.1 70.0 Case 73 Slot High x4 / Low x2 15 18.8 65.8 21.1 70.0 Case 14 Low Ind Ceiling 15 20.7 69.3 21.1 70,0 Case 12 Slot Ceiling 15 20.7 69.3 21.1 70.0 Case 64 Low Ind Low 20 18.9 66.0 21.2 70.1 Case 86 Slot Low 15 17.5 63.5 21.2 70.1 Case 29 Low Ind Low 5 11 51.8 21.2 70.1 Case 15 Low Ind Low 15 20.7 69.3 21.2 70.2 Case 68 Low Ind Low 20 18,9 66.0 21.2 70.2 Case 22 Radial Low 15 18.8 65.8 21.2 70.2 Case 69 Radial High x4 / Low x4 15 18,8 65.8 21.3 70.3 Case 82 Radial 2 Door exhausts 15 18.8 65.8 21.3 70.3 Case 11 Radial Low 15 20.7 69.3 21.3 70.4 Case 31 Radial Low 15 17.5 63.5 21.4 70.5 Case 83 Slot 2 Door exhausts 15 17.5 63,5 21.4 70.6 Case 70 Slot High x4 / Low x4 15 18.8 65.8 21.4 70.6 Case 84 Low Ind 2 Door exhausts 15 17.5 63.5 21.5 70.7 Case 71 Low Ind High x4 / Low x4 15 18.8 65.8 21.6 70,8 Case 57 Radial Low 15 18.8 65.8 21.7 71.1 Case 23 Slot Low 15 18.8 65.8 21.8 71.2 Case 58 Slot High 15 18.8 65.8 21.8 71.2 Case 79 Radial 2 Door exhausts 15 18.8 65.8 21.9 71.5 Case 32 Slot Low 15 17.5 63.5 22.0 71.5 Case 33 Low Ind Low 15 17.5 63.5 22.0 71.5 Case 61 Low Ind Low 15 18.8 65.8 22.0 71.7 Case 03 Radial Low 15 18.8 65.8 22.1 71.7 Case 80 Slot 2 Door exhausts 15 18.8 65.8 22.1 71.8 Case 24 Low Ind Low 15 18.8 65.8 22. 1 71.8 Case 06 Slot Low 15 18.8 65.8 22.3 72.1 Case 28 Low Ind Low 10 16.8 62.2 22.3 72.1 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 22.5 72.4 Case 87 Radial Low 15 19.2 66.6 22.5 72.4 Case 88 Slot Low 15 19.2 66.6 22.6 72.6 Case 09 Low Ind Low 15 18.8 65,8 22.6 72.6 Case 30 Low Ind Low 20 19.8 67.6 22.6 72.6 Case 89 Low Ind Low 15 19.2 66.6 22.9 73.3 Volume II -40 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 3.02 Ranking of Cases with respect to Cage Temperature Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°0 Supply Temperature (°F) Cage Temp rc ) Cage Temp (“F) Case 66 Low Ind Low 5 6,6 43.9 20.6 69.1 Case 62 Low Ind Low 5 6.6 43.9 20.9 69.6 Case 35 Slot Ceiling 15 17.5 63.5 21.3 70.4 Case 34 Radial Ceiling 15 17.5 63.5 21.4 70.4 Case 36 Low Ind Ceiling 15 17.5 63.5 21.5 70.6 Case 55 Low Ind Ceiling 15 18.8 65.8 21.6 70.9 Case 97 Slot Ceiling 15 17.5 63.5 21.6 71.0 Case 54 Slot Ceiling 15 18.8 65.8 21.7 71.0 Case 53 Radial Ceiling 15 18.8 65.8 21.7 71.0 Case 98 Low ind Ceiling 15 17.5 63.5 21.7 71.1 Case 19 Radial Ceiling 15 18.8 65.8 21.7 71.1 Case 96 Radial Ceiling 15 17.5 63.5 21.8 71.2 Case 94 Slot Ceiling 15 17.5 63.5 21.9 71.3 Case 67 Low Ind Low 10 14.8 58.6 21.9 71.4 Case 75 Radial Ceiling/Low 50/50 15 18.8 65.8 21.9 71.4 Case 60 Low Ind High 15 18.8 65.8 21.9 71.4 Case 63 Low Ind Low 10 14.8 58.6 21.9 71.4 Case 90 Radial Ceiling 15 18.8 65.8 21,9 71.5 Case 95 Low Ind Ceiling 15 17.5 63.5 21.9 71.5 Case 93 Radial Ceiling 15 17.5 63.5 21.9 71.5 Case 92 Low Ind Ceiling 15 18.8 65.8 22.0 71.6 Case 91 Slot Ceiling 15 18.8 65.8 22.0 71.6 Case 43 Low Ind (rot 90°) Ceiling 15 18.8 65.8 22.0 71.6 Case 46 Radial Ceiling 15 18.8 65.8 22.0 71.6 Case 21 Low Ind Ceiling 15 18.8 65.8 22.0 71.6 Case 59 Slot Low 15 18.8 65.8 22.0 71.6 Basecase Radial Ceiling 15 18.8 65.8 22.1 71.7 Case 18 Low Ind Ceiling 15 18.8 65.8 22.1 71.7 Case 45 Radial Ceiling 15 18.8 65.8 22.1 71.7 Case 17 Slot Ceiling 15 18.8 65.8 22.1 71.7 Case 20 Slot Ceiling 15 18.8 65.8 22.1 71.7 Case 47 Radial Ceiling 15 18.8 65.8 22. 1 71.7 Case 78 Low Ind Ceiling 15 18.8 65.8 22.1 71.8 Case 56 Radial High 15 18.8 65.8 22.1 71.8 Case 27 Low Ind Ceiling 15 18.8 65.8 22.1 71.8 Case 48 Radial Ceiling 15 18.8 65.8 22.1 71.8 Case 76 Radial Ceiling 15 18.8 65.8 22.1 71.8 Case 26 Slot Ceiling 15 18.8 65.8 22.1 71.8 Case 77 Slot Ceiling 15 18.8 65.8 22.1 71.9 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 22.2 71.9 Case 04 Slot Ceiling 15 18.8 65.8 22.2 71.9 Case 50 Radial Ceiling 15 18.8 65.8 22.2 71.9 Case 49 Radial Ceiling 15 18.8 65.8 22.2 71.9 Case 40 Radial Ceiling x4 15 18.8 65.8 22.2 71.9 Case 42 Low Ind Ceiling x4 15 18.8 65.8 22.2 71.9 Case 51 Radial Ceiling 15 18.8 65.8 22.2 71.9 Case 25 Radial Ceiling 15 18.8 65.8 22.2 72.0 Case 83 Slot 2 Door exhausts 15 17.5 63.5 22.2 72.0 Case 08 Low Ind High 15 18.8 65,8 22.2 72.0 Case 41 Slot Ceiling x4 15 18.8 65,8 22.2 72.0 Case 37 Radial Ceiling/Low 50/50 15 18.8 65.8 22.3 72.1 Case 05 Slot High 15 18.8 65.8 22.3 72.1 Case 82 Radial 2 Door exhausts 15 18.8 65.8 22.3 72.1 Case 86 I Slot Low 15 17.5 63.5 22.3 72.1 Volume II - Section III - Rankings of Results of Whole Room Runs Paee II - 41 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Cage Temp (°C) Cage Temp (°F) Case 02 Radial High 15 18.8 65.9 22.3 72.2 Case 85 Radial Low 15 17.5 63.5 22.4 72.3 Case 38 Slot Ceiling/Low 50/50 15 18.8 65.8 22.4 72.3 Case 39 Low Ind Ceiling/Low 50/50 15 18.8 65.8 22.4 72.3 Case 65 Low Ind Low 15 17.5 63.5 22.4 72.4 Case 07 Low Ind Ceiling 15 18.8 65.8 22.5 72.4 Case 52 Radial Ceiling 15 18.8 65.8 22.5 72.5 Case 22 Radial Low 15 18.8 65.8 22.5 72.5 Case 84 Low Ind 2 Door exhausts 15 17.5 63.5 22.5 72.5 Case 72 Radial High x4 / Low x2 15 18.8 65.8 22.5 72.6 Case 73 Slot High x4 / Low x2 15 18.8 65.8 22.6 72.7 Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 22.6 72.7 Case 29 Low Ind Low 5 II 51.8 22.6 72.8 Case 87 Radial Low 15 19.2 66.6 22.7 72.8 Case 64 Low Ind Low 20 189 66.0 22.7 72.8 Case 31 Radial Low 15 17.5 63.5 22.7 72.8 Case 23 Slot Low 15 18.8 65.8 22.7 72.9 Case 69 Radial High x4 / Low x4 15 18.8 65.8 22.7 72.9 Case 11 Radial Low 15 20.7 69.3 22.7 72.9 Case 68 Low Ind Low 20 18.9 66.0 22.8 73.0 Case 13 Slot Low 15 20.7 69.3 22.8 73.0 Case 14 Low Ind Ceiling 15 20.7 69.3 22.8 73.0 Case 16 Radial Ceiling 15 18.8 65.8 22.9 73.1 Case 70 Slot High x4 / Low x4 15 18.8 65.8 22.9 73.2 Case 32 Slot Low 15 17.5 63.5 22.9 73.2 Case 12 Slot Ceiling 15 20.7 69.3 22.9 73.2 Case 71 Low Ind High x4 / Low x4 15 18.8 65.8 23.0 73.3 Case 57 Radial Low 15 18.8 65.8 23.0 73.4 Case 58 Slot High 15 18.8 65.8 23.1 73.5 Case 79 Radial 2 Door exhausts 15 18.8 65.8 23.1 73.6 Case 61 Low Ind Low 15 18.8 65.8 23.1 73.6 Case 33 Low Ind Low 15 17.5 63.5 23.2 73.7 Case 24 Low Ind Low 15 18.8 65.8 23.2 73.7 Case 80 Slot 2 Door exhausts 15 18.8 65.8 23.2 73.8 Case 10 Radial Ceiling 15 20.7 69.3 23.2 73.8 Case 15 Low Ind Low 15 20.7 69.3 23.2 73.8 Case 28 Low Ind Low 10 16.8 62.2 23.3 74.0 Case 03 Radial Low 15 18.8 65.8 23.4 74.0 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 23.4 74.1 Case 06 Slot Low 15 18.8 65.8 23.4 74.2 Case 88 Slot Low 15 19.2 66.6 23.5 74.4 Case 09 Low Ind Low 15 18.8 65.8 23.6 74.5 Case 30 Low Ind Low 20 19.8 67.6 23.6 74.6 Case 89 Low Ind Low 15 19.2 66.6 23.7 74.6 Volume II -42 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 3.03 Ranking of Cases with respect to Room Relative Humidity Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room RH ( percent) Case 89 Low Ind Low 15 19.2 66.6 47.1 Case 88 Slot Low 15 19,2 66.6 48.1 Case 30 Low Ind Low 20 19.8 67.6 48.2 Case 09 Low Ind Low 15 18.8 65.8 48.4 Case 87 Radial Low 15 19.2 66.6 48.4 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 48.7 Case 06 Slot Low 15 18.8 65.8 49.2 Case 28 Low Ind Low 10 16.8 62.2 49.5 Case 03 Radial Low 15 18.8 65.8 49.7 Case 24 Low Ind Low 15 18.8 65.8 49.8 Case 80 Slot 2 Door exhausts 15 18.8 65.8 49.8 Case 61 Low Ind Low 15 18.8 65.8 49.8 Case 79 Radial 2 Door exhausts 15 18.8 65.8 50.3 Case 32 Slot Low 15 17.5 63.5 50.5 Case 58 Slot High 15 18.8 65.8 50.6 Case 33 Low Ind Low 15 17.5 63.5 50.7 Case 23 Slot Low 15 18.8 65.8 50.7 Case 57 Radial Low 15 18.8 65,8 50.7 Case 71 Low Ind High x4 / Low x4 15 18.8 65.8 51.2 Case 70 Slot High x4 / Low x4 15 18.8 65.8 51.4 Case 11 Radial Low 15 20.7 69.3 52.0 Case 69 Radial High x4 / Low x4 15 18.8 65.8 52.2 Case 31 Radial Low 15 17.5 63.5 52.3 Case 15 Low Ind Low 15 20.7 69.3 52.3 Case 84 Low Ind 2 Door exhausts 15 17.5 63.5 52,3 Case 22 Radial Low 15 18.8 65.8 52.3 Case 12 Slot Ceiling 15 20.7 69.3 52.3 Case 73 Slot High x4 / Low x2 15 18.8 65.8 52.6 Case 83 Slot 2 Door exhausts 15 17.5 63,5 52.6 Case 68 Low Ind Low 20 18.9 66.0 52.7 Case 14 Low Ind Ceiling 15 20.7 69.3 52.7 Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 52.7 Case 75 Radial Ceiling/Low 50/50 15 18.8 65.8 52.7 Case 82 Radial 2 Door exhausts 15 18.8 65.8 52.8 Case 72 Radial High x4 / Low x2 15 18,8 65.8 52.8 Case 64 Low Ind Low 20 18.9 66.0 52.9 Case 86 Slot Low 15 17.5 63.5 53.1 Case 10 Radial Ceiling 15 20.7 69.3 53.1 Case 29 Low Ind Low 5 11 51.8 53.2 Case 13 Slot Low 15 20.7 69.3 53.5 Case 38 Slot Ceiling/Low 50/50 15 18.8 65.8 53.9 Case 39 Low Ind Ceiling/Low 50/50 15 18.8 65.8 54.0 Case 37 Radial Ceiling/Low 50/50 15 18.8 65.8 54.2 Case 02 Radial High 15 18.8 65.8 54,3 Case 40 Radial Ceiling x4 15 18.8 65.8 54.4 Case 65 Low Ind Low 15 17,5 63.5 54.4 Case 48 Radial Ceiling 15 18.8 65.8 54.5 Case 49 Radial Ceiling 15 18.8 65.8 54.6 Case 05 Slot High 15 18.8 65.8 54.6 Case 41 Slot Ceiling x4 15 18.8 65.8 54.6 Case 85 Radial Low 15 17.5 63.5 54.7 Case 20 Slot Ceiling 15 18.8 65.8 54.8 Case 42 Low Ind Ceiling x4 15 18.8 65.8 54.8 Case 51 Radial Ceiling 15 18.8 65.8 54.8 Volume II - Section III - Rankings of Results of Whole Room Runs Paee II - 43 Case Name Supply DiffOser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room RH ( percent) Case 50 Radial Ceiling 15 18.8 65.8 54.9 Case 91 Slot Ceiling 15 18.8 65.8 55.0 Case 08 Low Ind High 15 18.8 65.8 55.0 Case 26 Slot Ceiling 15 18.8 65.8 55.0 Case 59 Slot Low 15 18.8 65.8 55.0 Case 52 Radial Ceiling 15 18.8 65.8 55.0 Case 92 Low Ind Ceiling 15 18.8 65.8 55.0 Case 78 Low bd Ceiling 15 18.8 65.8 55.1 Case 04 Slot Ceiling 15 18.8 65.8 55.1 Case 77 Slot Ceiling 15 18.8 65.8 55.2 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 55.2 Case 76 Radial Ceiling 15 18.8 65.8 55.2 Case 21 Low bd Ceiling 15 18.8 65.8 55.2 Case 90 Radial Ceiling 15 18.8 65.8 55.4 Basecase Radial Ceiling 15 18.8 65.8 55.4 Case 56 Radial High 15 18.8 65.8 55.5 Case 47 Radial Ceiling 15 18.8 65.8 55.5 Case 27 Low bd Ceiling 15 18.8 65.8 55.5 Case 25 Radial Ceiling 15 18.8 65.8 55.6 Case 45 Radial Ceiling 15 18.8 65.8 55.6 Case 60 Low bd High 15 18.8 65.8 55.6 Case 43 Low bd (rot 90°) Ceiling 15 18.8 65.8 55.8 Case 95 Low bd Ceiling 15 17.5 63.5 55.9 Case 46 Radial Ceiling 15 18.8 65.8 55.9 Case 19 Radial Ceiling 15 18.8 65.8 55.9 Case 93 Radial Ceiling 15 17.5 63.5 55.9 Case 17 Slot Ceiling 15 18.8 65.8 56.0 Case 18 Low bd Ceiling 15 18.8 65.8 56.0 Case 16 Radial Ceiling 15 18.8 65.8 56.2 Case 94 Slot Ceiling 15 17.5 63.5 56.3 Case 07 Low bd Ceiling 15 18.8 65.8 56.7 Case 63 Low bd Low 10 14.8 58.6 56.8 Case 98 Low ind Ceiling 15 17.5 63.5 56.9 Case 96 Radial Ceiling 15 17.5 63.5 57.0 Case 97 Slot Ceiling 15 17.5 63.5 57.0 Case 67 Low bd Low 10 14.8 58.6 57.2 Case 35 Slot Ceiling 15 17.5 63.5 57.4 Case 55 Low bd Ceiling 15 18.8 65.8 57.4 Case 54 Slot Ceiling 15 18.8 65.8 57.4 Case 53 Radial Ceiling 15 18.8 65.8 57.5 Case 34 Radial Ceiling 15 17.5 63.5 57.5 Case 36 Low bd Ceiling 15 17.5 63.5 57.7 Case 62 Low bd Low 5 6.6 43.9 62.6 Case 66 Low bd Low 5 6.6 43.9 64.4 Volume II -44 Ventilation Desi 2 n Handbook on Animal Research Facilities Using Static Microisolators Table 3.04 Ranking of Cases with respect to Cage RH Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (“F) Cage RH ( percent) Case 62 Low Ind Low 5 6.6 43.9 52.8 Case 66 Low Ind Low 5 6.6 43.9 53.0 Case 84 Low Ind 2 Door exhausts 15 17.5 63.5 53.0 Case 29 Low Ind Low 5 11 51.8 55.8 Case 87 Radial Low 15 19.2 66.6 56.3 Case 64 Low Ind Low 20 18.9 66.0 56.5 Case 23 Slot Low 15 18.8 65.8 56.9 Case 69 Radial High x4 / Low x4 15 18.8 65.8 57.1 Case 68 Low Ind Low 20 18.9 66.0 57.5 Case 32 Slot Low 15 17.5 63.5 57.5 Case 57 Radial Low 15 18.8 65.8 58.3 Case 79 Radial 2 Door exhausts 15 18.8 65.8 58.6 Case 33 Low Ind Low 15 17.5 63.5 58.7 Case 24 Low bd Low 15 18.8 65.8 58.7 Case 80 Slot 2 Door exhausts 15 18.8 65.8 59.1 Case 28 Low Ind Low 10 16.8 62.2 59.1 Case 03 Radial Low 15 18.8 65.8 60.2 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 60.2 Case 06 Slot Low 15 18.8 65.8 60.2 Case 88 Slot Low 15 19.2 66.6 60.4 Case 09 Low Ind Low 15 18.8 65.8 60.6 Case 30 Low Ind Low 20 19.8 67.6 60.9 Case 89 Low Ind Low 15 19.2 66.6 60.9 Case 22 Radial Low 15 18.8 65.8 61.1 Case 31 Radial Low 15 17.5 63.5 61.4 Case 58 Slot High 15 18.8 65.8 61.4 Case 61 Low Ind Low 15 18.8 65.8 61.6 Case 71 Low Ind High x4 / Low x4 15 18.8 65.8 61.6 Case 10 Radial Ceiling 15 20.7 69.3 61.7 Case 15 Low Ind Low 15 20.7 69.3 61.7 Case 70 Slot High x4 / Low x4 15 18.8 65.8 61.8 Case 82 Radial 2 Door exhausts 15 18.8 65.8 61.9 Case 72 Radial High x4 / Low x2 15 18.8 65.8 61.9 Case 86 Slot Low 15 17.5 63.5 62.0 Case 02 Radial High 15 18.8 65.8 62.0 Case 41 Slot Ceiling x4 15 18.8 65.8 62.3 Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 62.3 Case 37 Radial Ceiling/Low 50/50 15 18.8 65.8 62.4 Case 38 Slot Ceiling/Low 50/50 15 18.8 65.8 62.4 Case 05 Slot High 15 18.8 65.8 62.5 Case 20 Slot Ceiling 15 18.8 65.8 62.6 Case 65 Low Ind Low 15 17.5 63.5 62.7 Case 04 Slot Ceiling 15 18.8 65.8 62.7 Case 42 Low Ind Ceiling x4 15 18.8 65.8 62.8 Case 92 Low Ind Ceiling 15 18.8 65.8 62.8 Case 85 Radial Low 15 17.5 63.5 62.8 Case 39 Low Ind Ceiling/Low 50/50 15 18.8 65.8 62.9 Case 73 Slot High x4 / Low x2 15 18.8 65.8 62.9 Case 90 Radial Ceiling 15 18.8 65.8 63.3 Case 91 Slot Ceiling 15 18.8 65.8 63.4 Case 11 Radial Low 15 20.7 69.3 63.6 Case 12 Slot Ceiling 15 20.7 69.3 63.6 Case 50 Radial Ceiling 15 18.8 65.8 63.7 Case 18 Low Ind Ceiling 15 18.8 65.8 63.8 Volume II - Section III - Rankings of Results of Whole Room Runs Page II - 45 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Cage RH ( percent) Case 08 Low Ind High 15 18.8 65.8 63.8 Case 17 Slot Ceiling 15 18.8 65.8 63.9 Case 16 Radial Ceiling 15 18.8 65.8 64.0 Case 76 Radial Ceiling 15 18.8 65.8 64.1 Case 21 Low Ind Ceiling 15 18.8 65.8 64.1 Case 95 Low Ind Ceiling 15 17.5 63.5 64.1 Case 77 Slot Ceiling 15 18.8 65.8 64.2 Case 14 Low Ind Ceiling 15 20.7 69.3 64.2 Case 97 Slot Ceiling 15 17.5 63.5 64.2 Case 94 Slot Ceiling 15 17.5 63.5 64.4 Case 56 Radial High 15 18.8 65.8 64.4 Case 43 Low Ind (rot 90°) Ceiling 15 18.8 65.8 64.6 Case 19 Radial Ceiling 15 18.8 65.8 64.6 Case 78 Low Ind Ceiling 15 18.8 65.8 64.7 Case 52 Radial Ceiling ■ 15 18.8 65.8 64.8 Case 40 Radial Ceiling x4 15 18.8 65.8 64.8 Case 45 Radial Ceiling 15 18.8 65.8 64.9 Case 75 Radial Ceiling/Low 50/50 15 18.8 65.8 64.9 Case 63 Low Ind Low 10 14.8 58.6 64.9 Case 93 Radial Ceiling 15 17.5 63.5 65.0 Case 47 Radial Ceiling 15 18.8 65.8 65.1 Case 49 Radial Ceiling 15 18.8 65.8 65.2 Case 46 Radial Ceiling 15 18.8 65.8 65.2 Case 51 Radial Ceiling 15 18.8 65.8 65.3 Case 35 Slot Ceiling 15 17.5 63.5 65.5 Case 67 Low Ind Low 10 14.8 58.6 65.8 Case 98 Low ind Ceiling 15 17.5 63.5 65.8 Case 96 Radial Ceiling 15 17.5 63.5 65.8 Case 54 Slot Ceiling 15 18.8 65.8 66.0 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 66.0 Case 48 Radial Ceiling 15 18.8 65.8 66.1 Case 07 Low Ind Ceiling 15 18.8 65.8 66.4 Case 13 Slot Low 15 20.7 69.3 66.5 Case 36 Low Ind Ceiling 15 17.5 63.5 66.5 Base case Radial Ceiling 15 18.8 65.8 66.8 Case 83 Slot 2 Door exhausts 15 17.5 63.5 66.8 Case 59 Slot Low 15 18.8 65.8 66.9 Case 34 Radial Ceiling 15 17.5 63.5 67.2 Case 55 Low Ind Ceiling 15 18.8 65.8 67.8 Case 53 Radial Ceiling 15 18.8 65.8 68.8 Case 60 Low Ind High 15 18.8 65.8 69.1 Case 25 Radial Ceiling 15 18.8 65.8 73.0 Case 27 Low Ind Ceiling 15 18.8 65.8 74.8 Case 26 Slot Ceiling 15 18.8 65.8 81.2 Volume II -46 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 3.05 Ranking of Cases with respect to Room CO2 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room CO 2 (ppm) Case 59 Slot Low 15 18.8 65.8 15 Case 12 Slot Ceiling 15 20.7 69.3 17 Case 40 Radial Ceiling x4 15 18.8 65.8 21 Case 70 Slot High x4 / Low x4 15 18.8 65.8 24 Case 10 Radial Ceiling 15 20.7 69.3 29 Case 26 Slot Ceiling 15 18.8 65.8 35 Basecase Radial Ceiling 15 18.8 65.8 36 Case 13 Slot Low 15 20.7 69.3 37 Case 16 Radial Ceiling 15 18.8 65.8 37 Case 73 Slot High x4 / Low x2 15 18.8 65.8 37 Case 47 Radial Ceiling 15 18.8 65.8 40 Case 92 Low Ind Ceiling 15 18.8 65.8 41 Case 46 Radial Ceiling 15 18.8 65.8 43 Case 87 Radial Low 15 19.2 66.6 45 Case 11 Radial Low 15 20.7 69.3 48 Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 48 Case 75 Radial Ceiling/Low 50/50 15 18.8 65.8 48 Case 88 Slot Low 15 19.2 66.6 48 Case 15 Low Ind Low 15 20.7 69.3 48 Case 72 Radial High x4 / Low x2 15 18.8 65.8 48 Case 05 Slot High 15 18.8 65.8 49 Case 77 Slot Ceiling 15 18.8 65.8 50 Case 41 Slot Ceiling x4 15 18.8 65.8 51 Case 69 Radial High x4 / Low x4 15 18.8 65.8 51 Case 71 Low Ind High x4 / Low x4 15 18.8 65.8 51 Case 49 Radial Ceiling 15 18.8 65.8 52 Case 14 Low Ind Ceiling 15 20.7 69.3 52 Case 45 Radial Ceiling 15 18.8 65.8 52 Case 50 Radial Ceiling 15 18.8 65.8 55 Case 76 Radial Ceiling 15 18.8 65.8 55 Case 78 Low Ind Ceiling 15 18.8 65.8 55 Case 60 Low Ind High 15 18.8 65.8 57 Case 07 Low Ind Ceiling 15 18.8 65.8 57 Case 55 Low Ind Ceiling 15 18.8 65.8 57 Case 91 Slot Ceiling 15 18.8 65.8 57 Case 57 Radial Low 15 18.8 65.8 57 Case 53 Radial Ceiling 15 18.8 65.8 58 Case 37 Radial Ceiling/Low 50/50 15 18.8 65.8 59 Case 19 Radial Ceiling 15 18.8 65.8 59 Case 54 Slot Ceiling 15 18.8 65.8 60 Case 25 Radial Ceiling 15 18.8 65.8 60 Case 22 Radial Low 15 18.8 65.8 61 Case 27 Low Ind Ceiling 15 18.8 65.8 61 Case 21 Low Ind Ceiling 15 18.8 65.8 62 Case 04 Slot Ceiling 15 18.8 65.8 62 Case 89 Low Ind Low 15 19.2 66.6 62 Case 38 Slot Ceiling/Low 50/50 15 18.8 65.8 62 Case 23 Slot Low 15 18.8 65.8 63 Case 42 Low Ind Ceiling x4 15 18.8 65.8 63 Case 30 Low Ind Low 20 19.8 67.6 64 Case 08 Low Ind High 15 18.8 65.8 64 Case 03 Radial Low 15 18.8 65.8 64 Case 48 Radial Ceiling 15 18.8 65.8 65 Case 02 Radial High 15 18.8 65.8 65 Volume II - Section III - Rankings of Results of Whole Room Runs Page II - 47 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room CO 2 (ppm) Case 52 Radial Ceiling 15 18.8 65.8 65 Case 43 Low Ind (rot 90°) Ceiling 15 18.8 65.8 65 Case 51 Radial Ceiling 15 18.8 65.8 67 Case 58 Slot High 15 18.8 65.8 68 Case 61 Low Ind Low 15 18.8 65.8 69 Case 56 Radial High 15 18.8 65.8 69 Case 95 Low Ind Ceiling 15 17.5 63.5 69 Case 39 Low Ind Ceiling/Low 50/50 15 18.8 65.8 71 Case 06 Slot Low 15 18.8 65.8 72 Case 90 Radial Ceiling 15 18.8 65.8 72 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 74 Case 20 Slot Ceiling 15 18.8 65.8 75 Case 17 Slot Ceiling 15 18.8 65.8 78 Case 18 Low Ind Ceiling 15 18.8 65.8 79 Case 79 Radial 2 Door exhausts 15 18.8 65.8 81 Case 24 Low Ind Low 15 18.8 65.8 81 Case 98 Low ind Ceiling 15 17.5 63.5 83 Case 93 Radial Ceiling 15 17.5 63.5 86 Case 09 Low Ind Low 15 18.8 65.8 88 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 89 Case 80 Slot 2 Door exhausts 15 18.8 65.8 90 Case 68 Low Ind Low 20 18.9 66.0 93 Case 64 Low Ind Low 20 18.9 66.0 93 Case 96 Radial Ceiling 15 17.5 63.5 100 Case 31 Radial Low 15 17.5 63.5 107 Case 97 Slot Ceiling 15 17.5 63,5 1 1 1 Case 28 Low Ind Low 10 16.8 62.2 1 13 Case 82 Radial 2 Door exhausts 15 18.8 65.8 1 16 Case 94 Slot Ceiling 15 17.5 63.5 1 17 Case 35 Slot Ceiling 15 17.5 63.5 1 22 Case 86 Slot Low 15 17.5 63,5 124 Case 32 Slot Low 15 17.5 63.5 125 Case 85 Radial Low 15 17.5 63.5 132 Case 65 Low Ind Low 15 17.5 63.5 133 Case 36 Low Ind Ceiling 15 17.5 63.5 134 Case 34 Radial Ceiling 15 17.5 63.5 136 Case 29 Low Ind Low 5 11 51.8 140 Case 33 Low Ind Low 15 17.5 63.5 143 Case 84 Low Ind 2 Door exhausts 15 17.5 63.5 152 Case 83 Slot 2 Door exhausts 15 17.5 63.5 167 Case 67 Low Ind Low 10 14.8 58.6 201 Case 63 Low Ind Low 10 14.8 58.6 215 Case 66 Low Ind Low 5 6.6 43.9 318 Case 62 Low Ind Low 5 6.6 43.9 342 Volume II -48 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 3.06 Ranking of Cases with respect to Cage CO2 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Cage COj (ppm) Case 87 Radial Low 15 19.2 66.6 1391 Case 88 Slot Low 15 19.2 66.6 1550 Case 03 Radial Low 15 18.8 65.8 1568 Case 92 Low Ind Ceiling 15 18.8 65.8 1631 Case 89 Low Ind Low 15 19.2 66.6 1636 Case 79 Radial 2 Door exhausts 15 18.8 65.8 1647 Case 23 Slot Low 15 18.8 65.8 1652 Case 06 Slot Low 15 18.8 65.8 1652 Case 20 Slot Ceiling 15 18.8 65.8 1656 Case 52 Radial Ceiling 15 18.8 65.8 1665 Case 22 Radial Low 15 18.8 65.8 1665 Case 82 Radial 2 Door exhausts • 15 18.8 65.8 1667 Case 30 Low Ind Low 20 19.8 67.6 1667 Case 90 Radial Ceiling 15 18.8 65.8 1673 Case 41 Slot Ceiling x4 15 18.8 65.8 1690 Case 02 Radial High 15 18.8 65,8 1703 Case 80 Slot 2 Door exhausts 15 18.8 65.8 1707 Case 04 Slot Ceiling 15 18.8 65.8 1709 Case 91 Slot Ceiling 15 18.8 65.8 1709 Case 37 Radial Ceiling/Low 50/50 15 18.8 65,8 1715 Case 97 Slot Ceiling 15 17.5 63.5 1725 Case 09 Low Ind Low 15 18.8 65.8 1725 Case 19 Radial Ceiling 15 18.8 65,8 1725 Case 42 Low Ind Ceiling x4 15 18.8 65.8 1728 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 1741 Case 05 Slot High 15 18.8 65.8 1742 Case 32 Slot Low 15 17.5 63.5 1747 Case 28 Low Ind Low 10 16.8 62.2 1749 Case 69 Radial High x4 / Low x4 15 18.8 65.8 1750 Case 83 Slot 2 Door exhausts 15 17.5 63.5 1756 Case 94 Slot Ceiling 15 17.5 63.5 1757 Case 35 Slot Ceiling 15 17,5 63.5 1761 Case 29 Low Ind Low 5 1 1 51.8 1764 Case 38 Slot Ceiling/Low 50/50 15 18.8 65.8 1776 Case 72 Radial High x4 / Low x2 15 18.8 65.8 1790 Case 18 Low Ind Ceiling 15 18.8 65.8 1791 Case 64 Low Ind Low 20 18.9 66.0 1808 Case 21 Low Ind Ceiling 15 18.8 65.8 1808 Case 17 Slot Ceiling 15 18.8 65.8 1815 Case 95 Low Ind Ceiling 15 17.5 63.5 1822 Case 93 Radial Ceiling 15 17.5 63.5 1822 Case 68 Low Ind Low 20 18.9 66.0 1838 Case 75 Radial Ceiling/Low 50/50 15 18.8 65.8 1839 Case 50 Radial Ceiling 15 18.8 65.8 1840 Case 39 Low Ind Ceiling/Low 50/50 15 18.8 65.8 1848 Case 84 Low Ind 2 Door exhausts 15 17.5 63.5 1853 Case 57 Radial Low 15 18.8 65.8 1855 Case 43 Low Ind (rot 90°) Ceiling 15 18.8 65.8 1855 Case 76 Radial Ceiling 15 18.8 65.8 1857 Case 54 Slot Ceiling 15 18.8 65.8 1869 Case 08 Low Ind High 15 18.8 65.8 1871 Case 36 Low Ind Ceiling 15 17.5 63.5 1878 Case 77 Slot Ceiling 15 18.8 65.8 1881 Case 24 Low Ind Low 15 18.8 65.8 1883 Volume II - Section III - Rankings of Results of Whole Room Runs Page II - 49 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Cage CO 2 (ppm) Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 1884 Case 56 Radial High 15 18.8 65.8 1886 Case 34 Radial Ceiling 15 17.5 63.5 1896 Case 86 Slot Low 15 17.5 63.5 1917 Case 78 Low Ind Ceiling 15 18.8 65,8 1919 Case 45 Radial Ceiling 15 18.8 65.8 1929 Case 85 Radial Low 15 17.5 63.5 1930 Case 31 Radial Low 15 17.5 63.5 1938 Case 46 Radial Ceiling 15 18.8 65.8 1943 Case 98 Low ind Ceiling 15 17.5 63.5 1955 Case 70 Slot High x4 / Low x4 15 18.8 65.8 1955 Case 96 Radial Ceiling 15 17.5 63.5 1957 Case 47 Radial Ceiling 15 18.8 65.8 1961 Case 73 Slot High x4 / Low x2 15 18.8 65.8 1966 Case 71 Low Ind High x4 / Low x4 15 18.8 65.8 1968 Case 33 Low Ind Low 15 17.5 63.5 1974 Case 40 Radial Ceiling x4 15 18.8 65.8 1975 Case 58 Slot High 15 18.8 65.8 1990 Case 65 Low Ind Low 15 17.5 63.5 2002 Case 49 Radial Ceiling 15 18.8 65.8 2025 Case 55 Low Ind Ceiling 15 18.8 65.8 2039 Case 61 Low Ind Low 15 18.8 65.8 2042 Case 51 Radial Ceiling 15 18.8 65.8 2045 Case 63 Low Ind Low 10 14.8 58.6 2081 Case 48 Radial Ceiling 15 18.8 65.8 2099 Case 11 Radial Low 15 20.7 69.3 2118 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 2118 Case 10 Radial Ceiling 15 20.7 69.3 2119 Case 15 Low Ind Low 15 20.7 69.3 2119 Case 67 Low Ind Low 10 14.8 58.6 2144 Basecase Radial Ceiling 15 18.8 65.8 2158 Case 59 Slot Low 15 18.8 65.8 2161 Case 12 Slot Ceiling 15 20.7 69.3 2208 Case 53 Radial Ceiling 15 18.8 65.8 2212 Case 16 Radial Ceiling 15 18.8 65.8 2213 Case 14 Low Ind Ceiling 15 20.7 69.3 2215 Case 66 Low Ind Low 5 6.6 43.9 2318 Case 07 Low Ind Ceiling 15 18.8 65.8 2321 Case 62 Low Ind Low 5 ■ 6.6 43.9 2325 Case 60 Low Ind High 15 18.8 65.8 2354 Case 13 Slot Low 15 20.7 69.3 2491 Case 25 Radial Ceiling 15 18.8 65.8 3008 Case 27 Low Ind Ceiling 15 18.8 65.8 3171 Case 26 Slot Ceiling 15 18.8 65.8 3975 Volume II -50 Ventilation Desien Handbook on Animal Research Facilities Using Static Microisolators Table 3.07 Ranking of Cases with respect to Room NH 3 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room NH .1 (day 4) (ppm) Case 59 Slot Low 15 18.8 65.8 0.04 Case 12 Slot Ceiling 15 20.7 69.3 0.04 Case 70 Slot High x4 / Low x4 15 18.8 65.8 0.06 Case 40 Radial Ceiling x4 15 18.8 65.8 0.06 Case 10 Radial Ceiling 15 20.7 69.3 0.07 Case 73 Slot High x4 / Low x2 15 18.8 65.8 0.09 Case 16 Radial Ceiling 15 18.8 65.8 0.10 Case 87 Radial Low 15 19.2 66.6 0.10 Case 92 Lx)w Ind Ceiling 15 18.8 65.8 0.10 Basecase Radial Ceiling 15 18.8 65.8 0.11 Case 13 Slot Low 15 20.7 69.3 0.11 Case 88 Slot Low 15 19.2 66.6 0.1 1 Case 47 Radial Ceiling 15 18.8 65.8 0.1 1 Case 15 Low Ind Low 15 20.7 69.3 0.1 1 Case 72 Radial High x4 / Low x2 15 18.8 65.8 0.11 Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 0.11 Case 69 Radial High x4 / Low x4 15 18.8 65.8 0.12 Case 46 Radial Ceiling 15 18.8 65.8 0.12 Case 71 Low Ind High x4 / Low x4 15 18.8 65.8 0.12 Case 05 Slot High 15 18.8 65.8 0.12 Case 41 Slot Ceiling x4 15 18.8 65.8 0.12 Case 11 Radial Low 15 20.7 69.3 0.12 Case 57 Radial Low 15 18.8 65.8 0.13 Case 75 Radial Ceiling/Low 50/50 15 18.8 65.8 0.13 Case 77 Slot Ceiling 15 18.8 65.8 0.13 Case 14 Low Ind Ceiling 15 20.7 69.3 0.14 Case 22 Radial Low 15 18.8 65.8 0.14 Case 89 Low Ind Low 15 19.2 66.6 0.14 Case 50 Radial Ceiling 15 18.8 65.8 0.14 Case 37 Radial Ceiling/Low 50/50 15 18,8 65.8 0.14 Case 23 Slot Low 15 18.8 65.8 0.14 Case 45 Radial Ceiling 15 18,8 65.8 0.14 Case 49 Radial Ceiling 15 18.8 65.8 0.14 Case 30 Low Ind Low 20 19.8 67.6 0.14 Case 03 Radial Low 15 18.8 65.8 0.14 Case 76 Radial Ceiling 15 18.8 65.8 0.14 Case 91 Slot Ceiling 15 18.8 65.8 0.14 Case 78 Low Ind Ceiling 15 18.8 65.8 0.15 Case 38 Slot Ceiling/Low 50/50 15 18.8 65.8 0.15 Case 04 Slot Ceiling 15 18.8 65.8 0.15 Case 02 Radial High 15 18.8 65.8 0.15 Case 42 Low Ind Ceiling x4 15 18.8 65.8 0.16 Case 58 Slot High 15 18.8 65.8 0.16 Case 19 Radial Ceiling 15 18.8 65.8 0.16 Case 61 Low Ind Low 15 18.8 65.8 0.16 Case 26 Slot Ceiling 15 18.8 65.8 0.16 Case 06 Slot Low 15 18.8 65.8 0.16 Case 21 Low Ind Ceiling 15 18.8 65.8 0.16 Case 07 Low Ind Ceiling 15 18.8 65.8 0.16 Case 08 Low Ind High 15 18.8 65.8 0.16 Case 54 Slot Ceiling 15 18.8 65.8 0.17 Case 43 Low Ind (rot 90°) Ceiling 15 18.8 65.8 0.17 Case 55 Low Ind Ceiling 15 18.8 65.8 0.17 Case 52 Radial Ceiling 15 18.8 65.8 0.18 Volume II - Section III - Rankings of Results of Whole Room Runs Paee 11-51 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Room NHj (day 4) (ppm) Case 39 Low Ind Ceiling/Low 50/50 15 18.8 65.8 0.18 Case 90 Radial Ceiling 15 18.8 65.8 0.18 Case 60 Low Ind High 15 18.8 65.8 0.18 Case 95 Low Ind Ceiling 15 17.5 63.5 0.18 Case 20 Slot Ceiling 15 18.8 65.8 0.18 Case 79 Radial 2 Door exhausts 15 18.8 65.8 0.18 Case 24 Low Ind Low 15 18.8 65.8 0.18 Case 56 Radial High 15 18.8 65.8 0.18 Case 48 Radial Ceiling 15 18.8 65.8 0.18 Case 51 Radial Ceiling 15 18.8 65.8 0.18 Case 53 Radial Ceiling 15 18.8 65.8 0.18 Case 09 Low Ind Low 15 18.8 65.8 0.20 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 0.20 Case 17 Slot Ceiling 15 18.8 65.8 0.20 Case 80 Slot 2 Door exhausts 15 18.8 65.8 0.20 Case 18 Low Ind Ceiling 15 18.8 65.8 0.20 Case 68 Low Ind Low 20 18.9 66.0 0.21 Case 64 Low Ind Low 20 18.9 66.0 0.21 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 0.21 Case 25 Radial Ceiling 15 18.8 65.8 0.22 Case 98 Low ind Ceiling 15 17.5 63.5 0.23 Case 93 Radial Ceiling 15 17.5 63.5 0.24 Case 27 Low Ind Ceiling 15 18.8 65.8 0.24 Case 31 Radial Low 15 17.5 63.5 0.24 Case 28 Low Ind Low 10 16.8 62.2 0.25 Case 82 Radial 2 Door exhausts 15 18.8 65.8 0.27 Case 32 Slot Low 15 17.5 63.5 0.28 Case 96 Radial Ceiling 15 17.5 63.5 0.28 Case 97 Slot Ceiling 15 17.5 63.5 0.29 Case 86 Slot Low 15 17.5 63.5 0.29 Case 94 Slot Ceiling 15 17.5 63.5 0.31 Case 29 Low Ind Low 5 1 1 51.8 0.32 Case 33 Low Ind Low 15 17.5 63.5 0.32 Case 85 Radial Low 15 17.5 63.5 0.33 Case 65 Low Ind Low 15 17.5 63.5 0.33 Case 35 Slot Ceiling 15 17.5 63.5 0.34 Case 84 Low Ind 2 Door exhausts 15 17.5 63.5 0.34 Case 36 Low Ind Ceiling 15 17.5 63.5 0.39 Case 34 Radial Ceiling 15 17.5 63.5 0.41 Case 83 Slot 2 Door exhausts 15 17.5 63.5 0.49 Case 67 Low Ind Low 10 14.8 58.6 0.57 Case 63 Low Ind Low 10 14.8 58.6 0.58 Case 66 Low Ind Low 5 6.6 43.9 0.72 Case 62 Low Ind Low 5 6.6 43.9 0.77 Volume II -52 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Table 3.08 Ranking of Cases with respect to Cage NH 3 (Day 4) Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Cage NHj (day 4) (ppm) Case 87 Radial Low 15 19.2 66.6 3.13 Case 88 Slot Low 15 19.2 66.6 3.49 Case 03 Radial Low 15 18.8 65.8 3.53 Case 89 Low Ind Low 15 19.2 66.6 3.68 Case 79 Radial 2 Door exhausts 15 18.8 65.8 3.70 Case 23 Slot Low 15 18.8 65.8 3,71 Case 06 Slot Low 15 18.8 65.8 3.72 Case 30 Low Ind Low 20 19.8 67.6 3.75 Case 22 Radial Low 15 18.8 65.8 3.77 Case 80 Slot 2 Door exhausts 15 18.8 65,8 3.84 Case 09 Low Ind Low 15 18.8 65.8 3.88 Case 81 Low Ind 2 Door exhausts 15 18.8 65.8 3.92 Case 82 Radial 2 Door exhausts 15 18.8 65.8 3.92 Case 32 Slot Low 15 17.5 63.5 3.93 Case 28 Low Ind Low 10 16.8 62.2 3.93 Case 69 Radial High x4 / Low x4 15 18.8 65.8 3.94 Case 29 Low Ind Low 5 1 1 51.8 3.97 Case 92 Low Ind Ceiling 15 18.8 65.8 4.02 Case 02 Radial High 15 18.8 65.8 4.03 Case 20 Slot Ceiling 15 18,8 65.8 4.04 Case 41 Slot Ceiling x4 15 18.8 65.8 4.06 Case 64 Low Ind Low 20 18.9 66.0 4.07 Case 37 Radial Ceiling/Low 50/50 15 18.8 65.8 4.13 Case 68 Low Ind Low 20 18.9 66.0 4.13 Case 84 Low Ind 2 Door exhausts 15 17,5 63.5 4.17 Case 57 Radial Low 15 18.8 65.8 4.17 Case 04 Slot Ceiling 15 18.8 65.8 4.18 Case 72 Radial High x4 / Low x2 15 18.8 65.8 4.22 Case 90 Radial Ceiling 15 18.8 65.8 4.22 Case 05 Slot High 15 18.8 65.8 4.23 Case 24 Low Ind Low 15 18.8 65,8 4.24 Case 42 Low Ind Ceiling x4 15 18.8 65,8 4.25 Case 38 Slot Ceiling/Low 50/50 15 18.8 65.8 4.28 Case 91 Slot Ceiling 15 18.8 65.8 4.33 Case 33 Low Ind Low 15 17,5 63.5 4,44 Case 31 Radial Low 15 17.5 63.5 4.45 Case 52 Radial Ceiling 15 18.8 65.8 4.49 Case 74 Low Ind High x4 / Low x2 15 18.8 65.8 4.53 Case 97 Slot Ceiling 15 17.5 63.5 4.54 Case 86 Slot Low 15 17.5 63.5 4.54 Case 71 Low Ind High x4 / Low x4 15 18.8 65,8 4.56 Case 58 Slot High 15 18.8 65.8 4.58 Case 39 Low Ind Ceiling/Low 50/50 15 18.8 65.8 4.58 Case 70 Slot High x4 / Low x4 15 18.8 65.8 4.58 Case 19 Radial Ceiling 15 18.8 65.8 4.61 Case 18 Low Ind Ceiling 15 18.8 65.8 4.62 Case 94 Slot Ceiling 15 17.5 63,5 4.66 Case 17 Slot Ceiling 15 18.8 65.8 4.71 Case 61 Low Ind Low 15 18.8 65.8 4.73 Case 21 Low Ind Ceiling 15 18.8 65.8 4.73 Case 50 Radial Ceiling 15 18.8 65.8 4.73 Case 85 Radial Low 15 17.5 63.5 4.76 Case 95 Low Ind Ceiling 15 17.5 63.5 4.77 Volume II - Section III - Rankings of Results of Whole Room Runs Page II - 53 Case Name Supply Diffuser Type Exhaust Location and Number Supply ACH Supply Temperature (°C) Supply Temperature (°F) Cage NHj (day 4) (ppm) Case 08 Lx)w Ind High 15 18.8 65.8 4.83 Case 76 Radial Ceiling 15 18.8 65.8 4.85 Case 73 Slot High x4 / Low x2 15 18.8 65.8 4.87 Case 65 Low Ind Low 15 17.5 63.5 4.90 Case 35 Slot Ceiling 15 17.5 63.5 4.90 Case 77 Slot Ceiling 15 18.8 65.8 4.94 Case 10 Radial Ceiling 15 20.7 69.3 4.94 Case 15 Low Ind Low 15 20.7 69.3 4.94 Case 43 Low Ind (rot 90°) Ceiling 15 18.8 65.8 4.96 Case 93 Radial Ceiling 15 17.5 63.5 4.97 Case 75 Radial Ceiling/Low 50/50 15 18.8 65.8 4.98 Case 56 Radial High 15 18.8 65.8 5.01 Case 83 Slot 2 Door exhausts 15 17.5 63.5 5.15 Case 78 Low Ind Ceiling 15 18.8 65.8 5.16 Case 66 Low Ind Low 5 6 6 43.9 5.21 Case 45 Radial Ceiling 15 18.8 65.8 5.22 Case 62 Low Ind Low 5 6.6 43.9 5.23 Case 54 Slot Ceiling 15 18.8 65.8 5.32 Case 40 Radial Ceiling x4 15 18.8 65.8 5.33 Case 46 Radial Ceiling 15 18.8 65.8 5.35 Case 47 Radial Ceiling 15 18.8 65.8 5.36 Case 11 Radial Low 15 20.7 69.3 5.42 Case 36 Low Ind Ceiling 15 17.5 63.5 5.44 Case 98 Low ind Ceiling 15 17.5 63.5 5.50 Case 96 Radial Ceiling 15 17.5 63.5 5.51 Case 49 Radial Ceiling 15 18.8 65.8 5.56 Case 51 Radial Ceiling 15 18.8 65.8 5.64 Case 63 Low Ind Low 10 14.8 58.6 5.64 Case 12 Slot Ceiling 15 20.7 69.3 5.66 Case 34 Radial Ceiling 15 17.5 63.5 5.66 Case 16 Radial Ceiling 15 18.8 65.8 5.76 Case 14 Low Ind Ceiling 15 20.7 69.3 5.82 Case 48 Radial Ceiling 15 18.8 65.8 5.98 Case 44 Radial (rot 90°) Ceiling 15 18.8 65.8 6.03 Case 67 Low Ind Low 10 14.8 58.6 6.03 Case 55 Low Ind Ceiling 15 18.8 65.8 6.22 Basecase Radial Ceiling 15 18.8 65.8 6.33 Case 59 Slot Low 15 18.8 65.8 6.38 Case 07 Low Ind Ceiling 15 18.8 65.8 6.71 Case 53 Radial Ceiling 15 18.8 65.8 7.02 Case 13 Slot Low 15 20.7 69.3 7.21 Case 60 Low Ind High 15 18.8 65.8 7.56 Case 25 Radial Ceiling 15 18.8 65.8 1 1.05 Case 27 Low Ind Ceiling 15 18.8 65.8 12.31 Case 26 Slot Ceiling 15 18.8 65.8 18.42 Volume II - Section IV - Whole Room Simulation Database Page II - 55 4. WHOLE ROOM SIMULATION DATABASE 4.1 Description of Simulation Data Entry The first page contains a listing of the room geometry and ventilation configuration together with a picture. Summary result data is then presented for both average rack and room breathing zones. Temperature and CO 2 are presented as single figures whilst NH 3 values are shown over a ten day period. Please refer to Volume I: Section 4. 1.2. 8 . 2 for an explanation of the relationship between NH 3 generation rates and cage relative humidity. The second page shows three histogram population distributions of temperature, contamination (concentration) and relative humidity in the cage occupied zone of all cages in the room. The histograms show the number of cages (frequency) which have values that fall in the range between two values marked on the horizontal axis with the upper-value indicated. For example, in the sample histogram on the next page the column labeled 22.2 indicates that just over 40 cages have temperatures between 22°C and 22.2°C. Volume II - 56 Ventilation Pesi 2 n Handbook on Animal Research Facilities Using Static Microisolators Cascname BasecasC Descriplion t'xhausi Eihaust "S Room cage and ventilation SuppK Supply Discharge Supply Discharge I \haust Configuration Temperature (°C) Rll C ..nripii ati.m Temperature (®0 RH arrangement Radial 18. » 61% Ceiling > Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room .NUce in R.K.m Pressurisation ON On v\all Single loss 21100 gr neg lOOcfm Analysis Results Cage Occupied Z^ine Tempenturc CO. RH “C (ppm) Mean 22 06 7! 70 2158 66 78% SD ruo 072 359 2 60®-'.. Max. 22 99 73 38 3133 75 U6“/. Cage Occupied Zone NHj (ppm) Dav t 2 3 4 S S 7 8 9 i9 Mean 1 15 2 18 3 37 6.33 10 of) 15.82 23 78 29 51 35.35 38.09 Max. 1 67 3.17 4 89 9 19 1547 22 96 34 53 42 84 51 32 55 30 Room Breathing Zone Teoste nature CO, RH »c (ppn> Mean 20 291 68 52 36 55.371',. S.D 0.46 0 82 20 Max. 21 78 71 20 1.37 R(Him Breathing Zone NHj (ppm) Dsv t 2 3 4 S 7 8 9 19 Mean U.02 0t>4 OOh 0 1 1 0 18 0 2(5 0.4U 049 0 59 O.o3 Max. 0 07 0 14 021 0 41) 0.f)8 1 01 1 51 I 88 2 25 243 J > J Cage occupied zone summary result data Room breathing zone summary result data 15 10 Sample histrogram population distribution Volume II - Section IV - Whole Room Simulation Database Page 11-57 4.2 Simulation Data Entries The following section presents data entries for each of the CFD animal research facility simulations. Volume II - 58 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename BaSCCaSC Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Temperature CO 2 RH (ppm) Mean 22.06 71.70 2T5S 0^ OC c S.D. 0.40 0.72 559 2.60% Max. 2199 73.38 3133 75.06% Cage Occupied Zone NHj (ppm) Day I 2 3 5 5 6 7 8 9 TO Mean 1.15 2.18 3.37 6.33 10.66 15.82 23.78 29.51 35.35 38.09 Max. 1.67 3.17 4.89 9t9 15.47 22.96 34.53 42.84 51.32 55.30 Room Breathing Zone Temperature CO 3 , RH T (ppm) Mean 20.291 68.52 36 55.37% 0.46 0.82 20 Max. 21.78 71.20 137 Room Breathing Zone NHj (ppm) Day 1 2 3 3 5 s 7 8 9 ID Mean 0.02 0.04 0.06 0.1 1 0.18 0.26 0.40 0.49 0.59 (I63 Max. 0.07 0.14 on 0.40 0.68 1.01 1.51 1.88 2.25 2.43 Volume II - Section IV - Whole Room Simulation Database Page II - 59 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NH, 1 785000 418 2 785000 795 3 785000 1225 4 785000 1922 5 785000 2848 6 785000 3443 7 785000 8409 8 785000 8483 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 60 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 02 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% High 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-61 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO, NHj 1 785000 410 2 785000 795 5 785(XX) 1225 4 785(XX) 1860 5 785(XX) TFTT 6 785000 3847 7 785000 5401 8 785(XK) 7122 9 785tXX) 9214 10 785(XX) 11384 Cage occupied zone average relative humidity (%) distribution Volume II -62 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 03 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Analysis Results ^agTn«:upietl Zone Temperature RH — T sp (ppm) Mean 22.06 71.71 64 49.73% S.D. 0.37 0.66 25 Max. 23.37 74.07 170 Room Breathing Zone NH3 (ppm) Day i 2 3 3 5 6 7 S' 5 IG Mean 0.03 0.06 0.10 0.14 0.20 0.28 (T3?i 0.52 (169 (I90 Max. 0.09 0.17 0.27 0.38 0.54 0.75 1.02 1.38 1.83 2.39 Volume II - Section IV - Whole Room Simulation Database Page 11-63 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO, NII3 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 7850OO 3443 7 785000 4712 8 785000 6356 785OO0 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II - 64 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 04 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On 7*ag?7yccupie^Ton^ Analysis Results Temperature — — RH — (ppm) Mean 20.42 68.76 62 55.14% S.D. 0.17 0.0 26 Max. 21.18 70.12 170 Room Breathing Zone NH3 (ppm) Day 1 2 3 3 5 6 7 8 9 ID VI can o.o5 0.06 0.10 0.15 0.23 0.32 0.46 0.60 0.77 04 Max. 0.09 0.17 0.26 0.42 0^2 0:59 1.27 1.65 TTO 2.56 Volume II - Section IV - Whole Room Simulation Database Page II - 65 Histogram Distributions Cage occupied zone average temperature (°C) distribution n n ri ri rt ri ri ri Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 785000 411 2 785000 795 J 785000 1225 4 785000 1922 5 785000 2888 6 785000 4114 7 785000 5857 8 785000 7629 9 785000 9725 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 66 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 05 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% High 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Temperature n p RH op (ppm) Mean 212B 72.10 1742 62.51% S.b. 0.25 0.45 276 2.09% Max. 22.86 73.15 2333 67.21% Cage Occupied Zone NH3 (ppm) T5ay 1 2 3 4 5 5 7 10 Mean 0.91 1.76 2.72 4.23 6.32 12.75 16.66 TT.31 26.14 Max. 1.22 2.36 3.64 5.67 8.46 12.03 17.07 22.30 28.53 34.99 Room Breathing Zone Volume II - Section IV - Whole Room Simulation Database Page 11-67 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO, Ml3 1 785000 411 2 783000 795 3 785000 1225 4 785000 1906 5 785000 2848 6 785000 4048 7 785000 5744 a 785000 7504 9 785000 9599 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II - 68 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 06 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On lied Zor Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 69 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO2 NH3 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 8 785000 6356 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 70 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 07 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Temperature ^0, RH ^ (ppm) Mean 19.96 67.93 57 56.73% S.D. 0.68 1.22 82 Max. 2220 71.96 371 Room Breathing Zone NH3 (ppm) 15ay i 2 3 3 5 5 7 g 5 Mean 0.03 0.06 0.09 0.16 0.27 0.41 0.61 0.76 (T9T (199 Max. 0.20 0.38 0.58 1.07 1.79 2.65 3.97 T94 194 6.46 Volume II - Section IV - Whole Room Simulation Database Page 11-71 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm ) Day COj NH3 1 785000 418 1 785000 795 5 785000 1225 4 785000 2270 5 785000 3791 6 785000 5612 7 785000 8409 8 785000 10466 9 785000 12587 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 72 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 08 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% High 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tage Occupied Zone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 73 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NH3 1 785000 415 2 785000 795 3 785000 1225 4 785000 2027 5 785000 c^ 0 6 785000 4565 7 785000 6625 8 785000 8483 0 785000 105^6 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 74 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 09 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone upiec Analysis Results Temperature CO, RH ^ cp (ppm) Mean 22.57 72 62 88 48.37% S.b. 0.60 1.08 31 Max. 23.76 74.76 277 Room Breathing Zone NH3 (ppm) Day 1 3 4 5 6 7 8 9 ID Mean 0.05 0l)9 0.14 0.20 0.28 ojS 0.53 0.71 OO 1.23 Max. 0.14 OS 03 02 0.88 1.21 1.66 2.24 IM Volume II - Section IV - Whole Room Simulation Database Page I! - 75 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO, NHj 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2485 6 785(')0(:) 3443 7 785000 4712 H 785000 6356 0 785OO0 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 76 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 10 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 20.7 54% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation OFF On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On TagTCccupieS^onT' Analysis Results Temperature CO, — RH — ^F (ppm) Mean 20.92 69.66 29 53.13% Js.b. 0.39 0.70 44 Max. 22.21 71.98 158 Room Breathing Zone NH3 (ppm) bay j 2 3 4 5 5 7 g 9 10 Mean 0.02 (I03 0.05 0.07 0.10 0.14 (Ti^ 0:25 0.33 UaI Max] 0.08 (Tre 0.25 0.37 0.53 0.75 1.05 1.39 1.81 T29 Volume II - Section IV - Whole Room Simulation Database Page II - 77 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO^ NHj 1 785000 410 2 785000 795 J 785000 1225 4 785000 l83i 5 785000 2655 6 785000 3728 7 785000 5148 8 785000 6897 0 785000 8987 10 785000 irm Cage occupied zone average relative humidity (%) distribution Volume II - 78 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 11 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 20.7 54% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation OFF On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On (a ge Uccupiel^onr Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 79 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NII3 1 7850OO 413 2 785000 795 3 785000 1225 4 785000 2008 5 7S500O UTT 6 785000 4486 7 785000 6491 8 785000 8334 785000 10436 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 80 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 12 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 20.7 54% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation OFF On wall Single 1050 21000 gr neg lOOcfm Analysis Results ( age OccupTe^Ton? Temperature RH (ppm) .Mean 21^1 73.24 2208 63.65% S.D. 07 07 m 2.7S% Max. UM 74.45 5024 69.07% Cage Occupied Zone NH3 (ppm) Hiy 1 2 3 3 5 5 7 8 9 lU Mean 1.16 2.23 3.45 5.66 OS 12.66 18.32 23.52 29.43 34.67 Max. 1.59 3.06 4.72 7.75 rrui 17.33 25.09 32.20 40.30 47.47 Room Breathing Zone Volume II - Section IV - Whole Room Simulation Database Page II -81 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO2 NH3 1 785000 413 2 785000 795 5 785000 1225 4 785000 2011 5 785000 3121 6 785000 4500 7 785000 6514 8 785000 8360 9 7850()0 10462 to 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II - 82 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 13 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 20.7 54% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation OFF On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage OccupTe^^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 83 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day COj NHj 1 7S5MU 4l3 2 735000 795 3 785000 1225 4 735600 2273 5 785000 5798 6 785000 5624 7 785000 8430 8 785000 10490 *) 7350OO 13611 10 785000 11384 Cage occupied zone average relative humidity (%) di3tribution Volume II - 84 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 14 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 20.7 54% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation OFF On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On T^ag^Ccajpie^^onT Analysis Results Temperature O p — RH — (ppm) Mean 21.12 70.01 52 52.68% S.D. 0.17 0.31 23 Max. 22.07 71.72 220 Room Breathing Zone NH3 (ppm) Day 1 2 3 5 5 5 7 8 9 ID iVlean 0.03 0.05 (ToB 0.14 0.21 0.31 (O? (OB 0.72 p bo Max. 0.12 0.22 (04 0.58 0.91 1.32 1.93 2.45 3.04 5.52 Volume II - Section IV - Whole Room Simulation Database Page II - 85 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NH3 1 785000 414 2 785000 795 i 785000 1225 4 785000 2061 5 785000 3249 6 785O0O 4713 7 785000 6877 8 785000 8763 9 785000 10869 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II - 86 Ventilation Design Handbook on Animal Research Facilities Using Static IMicroisolators Casename Case 15 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 20.7 54% Low 22 50% Change Rack Rack Number of fotal mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation OFF On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Fop On "^ag^ncajpiet^onT Analysis Results Temperature — — RH — T T (ppm) Mean 2331 73,84 TiO 61.71% S.D. 08 (I5l 376 3.14% Max. 23.88 74,98 3201 68.32% Cage Occupied Zone NH3 (ppm) Day 1 2 3 4 5 6 7 S 9 Mean 1.11 2.14 3.31 4,94 IM 10.06 14.03 18.61 24.25 30.72 Max. 1.67 3.24 5.00 7.47 10.83 15.20 21.20 28.12 36.65 46.42 Room Breathing Zone Temperature co; RH T (ppm) Mean 21.21 70.19 48 52.32% S.D. 0.16 0.29 44 Max. 21.94 71.50 306 Room Breathing 2^ne NH3 (ppm) Day 1 2 3 4 5 6 7 8 9 jg Mean 0.03 0.05 0.07 0.1 1 0.16 0.23 0.32 0.42 0.55 otO Max. 0.3 1 0.48 0.71 1.03 1.45 2.02 2.69 3.50 4.43 Volume II - Section IV - Whole Room Simulation Database Page 11-87 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NH 3 1 785000 410 2 785000 795 3 785000 1225 4 785000 1832 5 78^000 2655 6 785000 3728 7 785000 5198 8 785000 6897 9 785000 8987 to 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 88 Ventilation Design Handbook on Animal Research Facilities Using Static IMicroisolators Casename Case 16 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr pos lOOcfm Room ACH 15 Cage Condition Top On Analysis Results Temperature CO, — RH — 0 n 0 T! (ppm) Mean 20.07 68.12 37 56.18% S.D. 129 2.33 47 Max. 23.06 73.52 251 Room Breathing Zone NH3 (ppm) Day I 2 3 4 5 6 7 g g 10 Mean 0.02 OT0 0.06 0.10 0.15 0.22 0.31 DaD (I50 DIE Max. 0.13 0.25 0.3^) 0.65 1.02 T4S 2.16 2.76 3.43 5I5D Volume II - Section IV - Whole Room Simulation Database Page 11-89 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Cage occupied zone average relative humidity (%) distribution Contamination conversion factors (kg/kg ppm) Volume II - 90 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 17 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr pos lOOcfm Room ACH 15 Cage Condition Top On Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 91 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Cage occupied zone average relative humidity (%) distribution Contamination conversion factors (kg/kg ^ ppm) Volume II - 92 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 18 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr pos lOOcfm Room ACH 15 Cage Condition Top On Cage OccupIecTZonT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-93 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day COj NHj i 785000 413 2 785000 795 3 785000 1225 4 785000 2024 5 785000 3152 6 785000 4552 7 785000 6603 8 785000 8458 *) 785000 10562 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 94 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 19 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On ^tge^orupied z!one Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 95 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 415 2 785000 795 5 785000 1225 4 785000 2100 5 785000 ~UW 6 785000 4879 7 785000 7160 8 785000 9078 f) 785000 1 1186 10 785000 TilP" Cage occupied zone average relative humidity (%) distribution 0 Volume II - 96 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 20 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage OccupTetTZonT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 97 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NH3 1 785000 411 2 785000 795 3 785000 1225 4 785O0O 1915 5 785000 "?S'7'1 ' 6 785000 4087 7 785000 5810 785000 7576 *) 785000 9672 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution 0 Volume II - 98 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 21 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Analysis Results Cage OccupieT^n^”” Volume II - Section IV - Whole Room Simulation Database Page 11-99 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 7k5000 414 2 785000 795 3 785000 1225 4 785000 :>054 5 785000 6 785000 4683 7 785000 6826 a 785000 8706 *) 785000 10811 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 100 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 22 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On (L'age ^iccupied Zone Analysis Results Temperature RH 5 ^ - Cp (ppm) Mean 21.24 70.24 61 52.34% S.b. 0.71 1.27 28 Max. 26:S2 80.28 217 Room Breathing Zone NH3 (ppm) Day 1 2 3 4 6 7 8 10 Mean 0.03 0.06 0.10 0.14 0.20 0.27 0.37 0.50 0.66 05 Max. 0.11 0.22 0.34 0.49 0.69 0.97 1.33 1.78 2.36 05 Volume II - Section IV - Whole Room Simulation Database Page II - 101 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 2 785000 705 3 785000 1225 4 785000 1779 5 785000 5516 6 785000 3498 7 785000 4807 8 785000 6461 9 785000 8548 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 102 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 23 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Analysis Results Temperature —TUT — RH — (ppm) Mean 72.86 1^52 60.16% S.D. (ITt 0.85 375 2.57% Max. 2535 74.38 2552 66.04% Cage Occupied Zone NH3 (ppm) T5ay 1 2 3 3 5^ 5 7 S 5 TO j Mean P bo O'. 1.67 2.58 3.71 5.22 7.24 Wi 13.37 17.76 23.22 Max. 1.35 23E 5.08 5.74 837 11.19 15.32 20.67 27.45 35.88 Room Breathing Zone Volume II - Section IV - Whole Room Simulation Database Page II - 103 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 785000 408 2 7})5000 705 3 7850OO \ns 4 785000 1766 5 785000 "ITST 6 7850O0 3445 7 7850OO 4712 H 785000 6356 !) 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II - 104 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 24 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Low 22 50% Change Raek Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Temf^rature —CO— RH ^ —sp (ppm) Mean 22.10 71.78 81 49,75% S.D. (Tts in 47 Max. TTtS 82.00 W Room Breathing Zone NH3 (ppm) Day ^71 2 3 4 6 7 8 g— - Mean O b 00 0.13 oTS 0.26 0.36 0.49 0.66 OS 1.15 Max. 0.22 0.42 0.67 0.06 1.35 1.87 2.56 3.46 430 on Volume II - Section IV - Whole Room Simulation Database Page 11-105 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day COj NHj 1 785000 o 00 2 785O00 795 i 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 8 785000 6356 *) 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 106 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 25 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Sealed TagiTncoIpie^^onr Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-107 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 429 2 785000 795 3 785000 1225 4 785000 2885 5 785000 5386 6 78500O 8256 7 785000 129lil 8 785000 15479 0 7^5000 17641 It) 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 108 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 26 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Sealed TagTOccupieTZon^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 109 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO 2 NII3 1 785000 443 2 785000 795 3 785000 1225 4 785000 3639 5 785000 7341 6 785000 1 1497 7 785000 18443 8 785000 21621 9 785000 23835 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 110 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 27 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Conflguration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Sealed Cage OccupIeTTonr" Analysis Results Temperature CO, — mr~ ^ !5p (ppm) Mean 20.31 68.56 61 55.54% S.b. 0:25 045 26 Max. 21.95 71.51 230 Room Breathing Zone NH3 (ppm) Day 1 2 3 4 5 6 7 8 9 10 Mean 0151 0.06 OTO 013 015 010 1.10 1.31 1.48 1.10 Max. 0.13 013 0.37 — oir~ 1.77 2.72 310 6A\ 5.77 510 Volume II - Section IV - Whole Room Simulation Database Page II- III Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day o o NHj 1 785000 432 2 785000 795 3 785000 1225 4 785000 3049 5 785000 5811 6 785000 8960 7 785000 14119 8 785000 16814 9 785000 18988 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume 11-112 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 28 Description Supply Conflguration Low Ind Supply Discharge Temperature (°C) 16.8 Supply Discharge RH 69% Exhaust Configuration Low Exhaust Exhaust Temperature (°C) RH 22 50% Change Station ON Rack Rack Number of Total mass of Room Orientation Density Mice in Room Mice in Room Pressurisation On wall Single 1050 21000 gr neg lOOcfm Room ACH 10 Cage Condition Top On Cage Occupied Zone Analysis Results Temj»rature CO, RH — — (ppm) Mean 22.28 72.10 113 49.45% S.D. (151 LTT) 29 Max. 531S 74.09 307 Room Breathing Zone NH3 (ppm) Day 3 4. ; :■ .-,5. : 7 , 7 S Mean 0.06 im O.U( (Il5 T05 (T5S nT~^ 1.59 Max. O.M 0.4S 0.69 0.97 1.35 1.84 — mr- — 05“ 4.31 Volume II - Section IV - Whole Room Simulation Database Page II- 113 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 8 785000 6356 9 785000 8442 10 785000 1 1384 0 Volume 11-114 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 29 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 11 65% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 5 Cage Condition Top On 7ageT5ccupie^^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 1 15 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 408 2 785000 795 3 7^5000 |755 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 7^5000 6356 9 7J)5000 §442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume 11-116 Ventilation Design Handbook on Animal Research Facilities Using Static Micro iso la tors Casename Case 30 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 19.8 57% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room Acn 20 Cage Condition Top On Tag^Hccupie^^on^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II- 117 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NH 3 1 785000 408 2 785000 795 3 785000 \115 4 785000 1766 5 7ii5oOO 2485 6 785000 3443 7 785000 4712 ii 785000 6356 9 785000 8442 10 7!)5000 11384 Cage occupied zone average relative humidity (%) distribution Volume II - 1 18 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 31 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 17.5 66% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Double 2100 42000 gr neg lOOcfm Analysis Results 7age^ccupTeT5Ionr Volume II - Section IV - Whole Room Simulation Database Page II - 119 Histogram Distributions Cage occupied zone average temperature (°C) distribution (N (N ppm) Day COj NH 3 1 785000 410 2 785000 795 3 785000 1755 4 785000 2346 5 785000 im' 6 785000 7 7S5000 8066 8 785000 11085 9 785000 13210 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -126 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 35 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 17,5 66% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tage^ccupietT^onT Analysis Results Temf^rature CO, RH T ^ (ppm) Mean 19.96 67.92 122 57.37% S.D. 1.01 l.$2 50 Max. 78.67 201 Room Breathing Zone NH3 (ppm) Day 1 2 3 5 6 7 8 Mean 0.06 (Til OTO 01^ 035 032 1.21 1.52 T35 2.06 Max. 0.15 0.20 0.45 0.81 1.32 1.05 2.80 150 53T Volume II - Section IV - Whole Room Simulation Database Page 11-127 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 416 1 785000 ~1W 3 785000 1225 4 785000 2185 5 785000 3571 6 785000 5246 7 7850OO 7787 H 785000 9774 785000 118^9 10 7})5O00 11384 Cage occupied zone average relative humidity (%) distribution Volume 11-128 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 36 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 17.5 66% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Petp Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On ^'age O caipie^Ton^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 129 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day COj NHj I 7i)5000 418 2 785000 795 3 7MDI5U ~T7IT 4 785000 2274 5 785000 3802 6 785000 5629 7 785000 8450 785O00 10500 9 785000 12620 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -130 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 37 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling/Low 50/50 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On ^ag^HmipIe^Ton^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 131 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 785000 411 2 785000 795 3 785000 1225 4 785000 1891 5 7H5O00 2808 6 785000 3983 7 785000 5632 8 785000 7379 7^<50()() 9473 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -132 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 38 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling/Low 50/50 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage OccupieT^one" Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 133 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 411 2 785000 795 3 785O0O 1725 4 785000 1807 5 785000 2811 6 785000 3086 7 785000 5630 s 785O00 7386 9 785000 9481 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -134 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 39 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Ceiling/Low 50/50 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On 7ag^5ccupie^^one Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 135 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj i 785000 4l7 2 785000 795 3 785000 15:55 4 785000 1044 5 7i)500O 5047 6 785000 4515 7 785000 6023 K 785000 781 5 9 785000 09l 1 to 785000 THM Cage occupied zone average relative humidity (%) distribution 0 Volume II -136 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 40 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Conflguration Temperature (°C) RH Radial 18.8 61% Ceiling x4 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^Cmipie^^on^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 137 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 415 2 785000 705 i 785000 1225 4 785000 2118 5 785000 3396 6 785000 4957 7 785000 7504 8 785000 9257 9 785000 11537 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -138 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 41 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling x4 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 139 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 78500() 411 2 785000 795 3 785000 1225 4 755UM 18f)5 5 785000 2701 6 785000 3054 7 785000 5584 & 785000 7325 785000 9419 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -140 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 42 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Ceiling x4 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^Occupie^^o upiec Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 141 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 7850OO 411 1 785000 705 3 785000 1225 4 785000 1930 5 7S5000 2910 6 785000 4151 7 78500O 5910 » 785000 769k 785000 TJW 10 7850O0 1 lt§4 Cage occupied zone average relative humidity (%) distribution Volume II -142 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 43 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind (rot 90°) 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage OmjpIeTTonT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-143 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 415 2 78500O 705 3 785000 1225 4 785000 2097 5 785000 3342 6 785000 4867 7 785000 7139 H 785000 9054 9 785000 11163 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -144 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 44 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial (rot 90°) 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Volume II — Section IV - Whole Room Simulation Database Page II - 145 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 417 2 785000 795 3 785000 1225 4 785000 2233 5 785000 3696 6 785000 5454 7 785000 8140 8 785000 10167 9 785000 12285 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -146 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 45 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg 50cfm Room ACH 15 Cage Condition Top On Tag^CccupieJTonr Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-147 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj .'I. 785000 415 2 785000 795 3 785000 1225 4 785000 2127 5 785000 3419 6 785000 4995 7 785000 7358 8 785000 9298 9 785000 11408 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -148 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 46 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Conflguration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neutral Room ACH 15 Cage Condition Top On TagTCccupIeS^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 149 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 416 2 785000 795 3 785000 1225 4 785000 2160 5 785000 3506 6 785000 5139 7 785000 7604 8 785000 9571 9 785000 11684 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -150 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 47 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr pos 50cfm Room Acn 15 Cage Condition Top On Tag^ncajpie^^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 151 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO 2 NHj 1 785000 415 2 785000 795 3 785000 1225 4 785000 2146 5 785000 3470 6 785000 5079 7 785000 7501 8 785000 9456 9 785000 11568 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -152 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 48 Description Supply Configuration Radial Supply Discharge Temperature (°C) 18.8 Supply Discharge RH 61% Exhaust Configuration Ceiling Exhaust Exhaust Temperature (°C) RH 22 50% Change Rack Rack Station Orientation Density ON (swapped with radDn wall Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On Tage^ccupIeTZon^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II- 153 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 417 2 785000 795 3 785000 1225 4 785000 2237 5 785000 3704 6 785000 5468 7 785000 8165 8 785000 10195 9 785000 12313 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -154 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 49 Description Supply Configuration Radial Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Temperature (°C) RH Configuration Temperature (°C) RH 18.8 61% Ceiling 22 50% Change Rack Rack Station Orientation Density ON (swapped with racOn wall Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On Tage^ccupieTTonT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 155 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO 2 NHj 1 785000 416 2 785000 795 3 785000 1225 4 785000 2156 5 785000 3496 6 785000 5123 7 785000 7576 8 785000 9540 9 785000 11652 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -156 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 50 Description Supply Configuration Radial Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Temperature (°C) RH Configuration Temperature (°C) RH 18.8 61% Ceiling 22 50% Change Rack Rack Station Orientation Density ON (swapped with racDn wall Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On TagTCmipieS^onr' Analysis Results Volume n - Section IV - Whole Room Simulation Database Page II -157 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 413 2 785000 795 3 785000 1225 4 785000 2019 5 785000 3139 6 785000 4531 7 785000 6567 8 785000 8418 9 785000 10521 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -158 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 51 Description Supply Configuration Radial Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Temperature (°C) RH Configuration Temperature (°C) RH 18.8 61% Ceiling 22 50% Change Rack Rack Station Orientation Density ON (swapped with racDn wall Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II- 159 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 416 2 785000 795 3 785000 1225 4 785000 2166 5 785000 3521 6 785000 5163 7 785000 7645 8 785000 9617 9 785000 1 1730 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -160 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 52 Description Supply Configuration Radial Supply Discharge Supply Discharge Temperature (°C) RH 18.8 61% Exhaust Configuration Ceiling Exhaust Exhaust Temperature (°C) RH 22 50% Change Rack Rack Station Orientation Density ON (swapped with radDn wall Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On Tage^ccupTeS^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 161 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 415 2 785000 795 3 785000 1225 4 785000 2118 5 785000 3396 6 785000 4956 7 785000 7292 8 785000 9224 9 785000 11334 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -162 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 53 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On T^ag^Cccupie^TonT Analysis Results Temperature COj — RB (ppm) Mean 19.77 67.59 58 57.45% S.D. 0.16 0.:58 24 Max. 21.55 70.80 168 Room Breathing Zone NH3 (ppm) Day I 2 3 4 5 6 7 S 9 IQ Mean p o 0.06 o.oO 0.1 8 0.32 0.40 0.74 (T9T TUt 1.10 Max. 0.17 UlE 0.53 0.93 1.40 2.14 TE2 JM 3.18 Volume II - Section IV - Whole Room Simulation Database Page 11-163 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO 2 NHj 1 785000 422 2 785000 795 3 785000 1225 4 785000 2490 5 785000 4362 6 785000 6558 7 785000 10023 8 785000 12260 9 785000 14396 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -164 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 54 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18,8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage OccupieJTon^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 165 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 417 2 785000 795 3 785000 1225 4 785000 2233 5 785000 3694 6 785000 5451 7 785000 8136 8 785000 10163 9 785000 12281 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -166 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 55 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18,8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On TagTCccupIeTTonT' Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 167 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO 2 NHj 1 785000 420 2 785000 795 3 785000 1225 4 785000 2395 5 785000 4115 6 785000 6148 7 785000 9324 8 785000 1 1483 9 785000 13612 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -168 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 56 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% High 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^HmlpIe^^on^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-169 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day o p NHj 1 785000 414 2 785000 795 3 785000 1225 4 785000 2084 5 785000 3309 6 785000 4812 7 785000 7047 8 785000 8952 9 785000 11059 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -170 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 57 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18,8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 171 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO 2 NHj I 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 8 785000 6356 9 785000 8442 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution 0 Volume 11-172 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 58 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% High 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^HcaipIe^Ton^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 173 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 409 2 785000 795 3 785000 1225 4 785000 1806 5 785000 2586 6 785000 3614 7 785000 5004 8 785000 6681 9 785000 8770 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -174 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 59 Description Supply Supply Discharge Supply Discharge Exh^st Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On tSginnicnipieTZJ upie Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 175 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO 2 NHj 1 785000 419 2 785000 795 3 785000 1225 4 785000 2318 5 785000 3916 6 785000 5819 7 785000 8763 S 785000 10860 9 785000 12984 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -176 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 60 Description Supply Configuration Low Ind Supply Discharge Temperature (°C) 18.8 Supply Discharge RH 61% Exhaust Configuration High Exhaust Exhaust Temperature (°C) RH 22 50% Change Station ON alt design Rack Orientation On wall Rack Density Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On TagrCccupien^onr" Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-177 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 422 2 785000 795 3 785000 1225 4 785000 2520 5 785000 4439 6 785000 6686 7 785000 10242 8 785000 12503 9 785000 14641 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -178 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 61 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On TagrT5ccup!eT2Ion^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 179 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO2 NH3 1 785000 409 2 785000 795 3 785000 1225 4 785000 1818 5 785000 2618 6 785000 3668 7 785000 5095 8 785000 6782 9 785000 8872 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -180 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 62 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 6.6 89% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Double 2100 42000 gr neg lOOcfm Room ACH 5 Cage Condition Top On Cage Occuple^^on^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 181 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 Nllj 1 785000 415 2 785000 795 3 785000 1225 4 785000 2129 5 785000 3425 6 785000 5004 7 785000 7374 8 785000 9315 9 785000 1 1426 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -182 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 63 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Conflguration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 14.8 79% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Double 2100 42000 gr neg lOOcfm Analysis Results TTag^Cccupie^^onT Volume II - Section IV - Whole Room Simulation Database Page II - 183 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO^ NHj 1 785000 415 2 785000 795 3 785000 1225 4 785000 2129 5 785000 3425 6 785000 5004 7 785000 7374 8 785000 9315 9 785000 11426 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -184 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 64 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.9 60% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Double 2100 42000 gr neg lOOcfm Analysis Results TagTCccupieTTon? Volume II - Section IV - Whole Room Simulation Database Page II - 185 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day O o NHj 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 8 785000 6356 9 785000 8442 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -186 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 65 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 17.5 66% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 187 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 411 2 785000 795 3 785000 1225 4 785000 1921 5 785000 2886 6 785000 41 1 1 7 785000 5850 8 785000 7622 9 785000 9718 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -188 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 66 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 6.6 89% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp Double 2100 42000 gr neg lOOcfm Room ACH 5 Cage Condition Top On T^geTJccupiedZoiie Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 189 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day o p NHj 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 8 785000 6356 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -190 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 67 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 14.8 79% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp Double 2100 42000 gr neg lOOcfm Room ACH 10 Cage Condition Top On TagTCccupien^onT Analysis Results Temperature — RH — T ^ (ppm) Mean 20.19 68.35 201 57.23% S.D. 0.52 0.93 90 Max. 21.51 70.72 623 Room Breathing Zone NH3 (ppm) Day I 2 3 4 5 6 7 8 9 10 Mean 0.11 TIU on 0.57 on 1.37 2M 2.55 3.10 3 . 42 ' Max. 0.33 0.63 (W7 1.75 4.25 6.32 7n 0:00 ion Volume II - Section IV - Whole Room Simulation Database Page II - 191 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 417 2 785000 795 3 785000 1225 4 785000 2209 5 785000 3633 6 785000 5350 7 785000 7963 8 785000 9970 9 785000 12087 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -192 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 68 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Conflguration Temperature (°C) RH Low Ind 18.9 60% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp Double 2100 42000 gr neg lOOcfm Room ACH 20 Cage Condition Top On Analysis Results Cage Occupied Zone Volume II - Section IV - Whole Room Simulation Database Page 11-193 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO 2 NHj 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 8 785000 6356 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -194 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 69 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Conflguration Temperature (°C) RH Conflguration Temperature (°C) RH Radial 18.8 61% High x4 / Low x4 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tage^iTcupIe^^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-195 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO 2 NHj 1 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 e 785000 3443 7 785000 4712 8 785000 6356 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -196 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 70 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% High x4 / Low x4 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On TagTCceuple^Ton^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-197 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO 2 NHj ,.t 785000 410 2 785000 795 3 785000 1225 4 785000 1839 5 785000 2673 C 785000 3758 7 785000 5250 8 785000 6954 9 785000 9045 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -198 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 71 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% High x4 / Low x4 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Analysis Results T^ag^CccupIe^^onT Volume II - Section IV - Whole Room Simulation Database Page 11-199 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 409 2 785000 795 3 785000 1225 4 785000 1821 5 785000 2626 6 785000 3680 7 785000 5116 8 785000 6805 9 785000 8894 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -200 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 72 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% High x4 / Low x2 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Analysis Results Tag^dmlple^^onr Volume II - Section IV - Whole Room Simulation Database Page II - 201 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ppm) Day CO2 NH3 1 785000 418 2 785000 795 3 785000 1225 4 785000 2303 5 785000 3876 6 785000 5752 7 785000 8650 8 785000 10734 9 785000 12856 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -202 Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 73 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% High x4 / Low x2 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tage^ccupIeTZonr" Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 203 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 785000 412 2 785000 795 3 785000 1225 4 785000 1947 5 785000 2952 6 785000 4221 7 785000 6038 8 785000 7831 9 785000 9929 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -204 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 74 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% High x4 / Low x2 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^nccupiet^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 205 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 785(M)0 41 1 2 785000 795 3 7^5000 1225 4 785000 IW 5 785000 2800 6 785000 3969 7 785000 5609 a 785000 7353 9 785000 9447 10 7ii5000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -206 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 75 Description Supply Configuration Radial Supply Discharge Temperature (°C) 18.8 Supply Discharge RH 61 % Exhaust Configuration Ceiling/Low 50/50 Exhaust Temperature (°C) 22 Exhaust RH 50% Change Station ON alt design Rack Orientation On wall Rack Density Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On TTag^Cccupie^TonT Analysis Results Temperature — UJT~ — RH — ^ cp (ppm) Mean 2 1 .09 69.96 48 52.74% S.b. (HI ^ 22 Max. 22.37 72.26 247 Room Breathing Zone NH3 (ppm) Day I 2 3 5 6 7 g 9 10 Mean 0.03 0.05 0T)7 (TTl — m — — OT“ 0715 0.57 urn Max. 0,1 3 0.25 0.30 OTt — n5s~ 1.57 Tn 193 3.60 Volume II - Section IV - Whole Room Simulation Database Page II - 207 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 7t^5O0O 415 2 785000 795 3 785000 1225 4 785000 2127 5 785000 3420 6 78500O 4996 1 785000 7360 8 785000 9 785000 TTTRJ 10 7S5000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -208 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 76 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Analysis Results iL'age i?5ccupled Zone Volume II - Section IV - Whole Room Simulation Database Page II - 209 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 7S5O0O 414 2 785000 795 3 785000 i:>55 4 785000 2049 5 785o0o 3218 785000 4662 7 785000 6791 H 785000 8667 9 785000 10772 10 7!)5000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -210 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 77 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Analysis Results TageTJcajpieT^nT Volume II - Section IV - Whole Room Simulation Database Page 11-211 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 78500O 414 2 785000 795 3 785000 1225 4 785000 >060 5 785000 5247 6 785000 4710 7 78$O00 6872 8 785000 8758 9 785000 10864 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -212 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 78 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH ConTiguration Temperature (°C) RH Low Ind 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage OccupTeTZonT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page 11-213 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 415 2 785O00 795 3 785000 1225 4 785000 2109 5 785000 ~i7rr 6 785000 4919 7 785000 7229 8 785000 9154 785000 11263 10 785O00 11584 Cage occupied zone average relative humidity (%) distribution Volume II -214 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 79 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% 2 Door exhausts 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On TagTC^upied itone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 215 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day COj NH 3 1 7i)5()00 408 1 785000 795 3 785000 1225 4 785000 1766 5 785000 2485 6 785000 7 785000 4712 8 7850O0 9 785000 8445 10 7S5000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -216 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 80 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% 2 Door exhausts 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On ^agTCccupIen^o upie Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 217 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NH 3 1 785OO0 40i} 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785O00 3443 7 785000 47 1 7 H 785000 6356 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -218 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 81 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 18.8 61% 2 Door exhausts 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On ^ag^CccupIeTZone upieo Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 219 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 408 2 785000 795 3 7J15000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 785000 6356 “9~ 785000 8442 ~nr 71)5000 11584 Cage occupied zone average relative humidity (%) distribution Volume II -220 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 82 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RII Configuration Temperature (°C) RH Radial 18.8 61 % 2 Door exhausts 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tage^ccupi^r^onr Volume II - Section IV - Whole Room Simulation Database Page II - 221 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 410 2 785000 795 3 785000 \ns 4 785000 1848 5 78?00O 2605 6 785000 3795 7 785000 5313 » 785000 7024 785000 9115 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -222 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 83 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Conflguration Temperature (°C) RH Slot 17.5 66% 2 Door exhausts 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupie^^nT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 223 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NH, 1 785000 418 1 785000 795 5 785000 1225 4 785000 7303 5 785000 3876 6 785000 5752 7 785000 8650 785000 10734 9 12856 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -224 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 84 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 17.5 66% 2 Door exhausts 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On 7ag^5ccupie3T^on^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 225 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 7$50O() 408 2 7i)5()O0 795 3 785000 1225 4 785000 1766 5 748? 6 7t)5()O0 344? 7 7t)5000 4712 a 7i)5000 6356 9 785000 8442 10 "7^'0()U 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -226 Design Handbook on Animal Research Facilities Using Static Microisolators Cusename Case 85 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Conflguration Temperature (°C) RH Conflguration Temperature (°C) RH Radial 17,5 66% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On TagTCccupietTronT' Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 227 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 4ll 2 7^5000 795 3 7i<5000 1225 4 785000 1935 5 785000 2922 6 7^5000 4171 7 785000 5950 8 785000 7736 785000 9833 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -228 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 86 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Conflguration Temperature (°C) RH Configuration Temperature (°C) RH Slot 17.5 66% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Dccupie^^n^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 229 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO 2 NHj 1 7Ji5000 4l0 2 785000 705 3 785000 12^5 4 785000 1859 5 78500O 2776 6 785000 3846 7 785000 5400 » 785000 7121 785000 9213 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -230 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 87 Description Supply Supply Diseharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Conflguration Temperature (°C) RH Radial 19.2 59% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Reduced 700 14000 gr neg lOOcfm Room ACH 15 Cage Condition Top On ^gTCccupIeTTonT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page ll - 231 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day CO^ NHj 1 7S5O0O 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 » 7j)5000 6356 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -232 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 88 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 19.2 59% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Reduced 700 14000 gr neg lOOcfm Analysis Results 7"ag^0mipieT^onr Volume II - Section IV - Whole Room Simulation Database Page II - 233 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day C 02 NHj 1 785O0O 40S 2 785000 795 3 785000 1225 4 785O00 1766 5 785000 2483 6 785O0O 7 785000 4712 a 785000 6356 9 785000 8442 10 785000 TUM Cage occupied zone average relative humidity (%) distribution Volume II -234 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 89 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Conflguration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 19.2 59% Low 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Reduced 700 14000 gr neg lOOcfm Room ACH 15 Cage Condition Top On 7ageT5ccupie^Ton^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 235 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj I 785000 408 2 785000 795 3 785000 1225 4 785000 1766 5 785000 248$ 6 785000 3443 7 785000 4712 a 785000 6356 785000 ~mr 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -236 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 90 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp all 5 on 1 wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On c’age Occupl^^one Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 237 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO^ NHj 1 785000 412 1 785OO0 795 3 785000 1225 4 785000 1982 5 785000 3043 6 7850O0 4371 7 785000 6295 it 785000 8116 9 785000 10216 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -238 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 91 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 18.8 61% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp all 5 on 1 wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^CcoIpIeT^on^ Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 239 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 785000 412 2 785000 795 3 785000 1225 4 785000 1987 5 785000 3056 6 785000 4394 7 785000 6333 8 785000 8158 785000 10259 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -240 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 92 Description Supply Configuration Low Ind Supply Discharge Temperature (°C) 18.8 Supply Discharge Exhaust RH Configuration 61% Ceiling Exhaust Exhaust Temperature (°C) RH 22 50% Change Station ON Rack Rack Number of Total mass of Room Orientation Density Mice in Room Mice in Room Pressurisation Perp all 5 on 1 wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 241 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 4l 1 2 785O0O 795 3 785000 1225 4 785000 5 7k5o0O 2916 6 785000 4161 7 785000 5936 8 7^5000 7716 785000 9814 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -242 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 93 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 17.5 66% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp all 5 on 1 wall Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tagrnccupie3Tonr" Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 243 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) E>ay COj NHj i 7850OO 415 2 785000 795 3 785000 1225 4 785000 :)i4i 5 785000 6 785000 5058 7 785000 7466 K 785000 94l8 9 785000 11529 10 785000 TUP’ Cage occupied zone average relative humidity (%) distribution Volume II -244 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 94 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 17.5 66% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp all 5 on 1 wall Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On TagTCcajp^enZonT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 245 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 414 2 7^5000 795 3 785000 1225 4 785000 OT 80 5 7850OO 5200 6 785000 4705 7 785000 7017 B 785000 8910 9 785000 11026 10 78 5 DOT 11584 Cage occupied zone average relative humidity (%) distribution Volume II -246 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 95 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 17.5 66% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON Perp all 5 on 1 wall Double 2100 42000 gr neg lOOcfm Analysis Results Tag^nccupie^TonT Volume II - Section IV - Whole Room Simulation Database Page II - 247 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 414 2 78500d 795 J 785000 1225 4 785OO0 2054 s 785000 3231 6 785000 4684 7 785000 6827 K 785000 "StUT 9 785000 10814 10 785000 TOM Cage occupied zone average relative humidity (%) distribution Volume II -248 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 96 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Radial 17.5 66% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp all 5 on 1 wall Double 2100 42000 gr neg lOOcfm Analysis Results Tage^coipIe3T5onr Volume II - Section IV - Whole Room Simulation Database Page II - 249 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 417 2 785000 795 3 785000 1225 4 785000 2210 5 785000 3636 6 785000 5355 7 785000 7972 8 785000 9 785O00 12097 10 785000 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -250 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 97 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Slot 17.5 66% Ceiling 22 50% Change Raek Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp all 5 on 1 wall Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Cage Occupied Zone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 251 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 414 2 785000 795 5 785000 1225 4 785000 2065 5 785000 3259 6 785000 4730 7 785O00 6907 8 785000 8796 9 785000 10902 10 785000 1 1384 Cage occupied zone average relative humidity (%) distribution Volume II -252 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 98 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low ind 17.5 66% Ceiling 22 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp all 5 on 1 wall Double 2100 42000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^nccupie^Tone Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 253 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg -> ppm) Day COj NHj 1 7i)5000 417 2 785000 795 5 785000 \ns 4 785000 5 785000 3636 6 785000 5355 7 785000 7971 a 785(K)0 9979 9 785000 12096 10 785006 11384 Cage occupied zone average relative humidity (%) distribution 0 Volume II -254 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 99 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Conflguration Temperature (°C) RH Radial 22 49% Ceiling 26 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON On wall Single 1050 21000 gr neg lOOcfm Room ACH 15 Cage Condition Top On Tag^Tccupiet^onT Analysis Results Volume II - Section IV - Whole Room Simulation Database Page II - 255 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 408 2 785000 795 3 7850OO 1^55 4 785000 1766 5 785000 2483 6 785000 3443 7 785000 4712 s 785000 6356 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume 11-256 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 100 Description Supply Configuration Radial Supply Discharge Supply Discharge Temperature (°C) RH 22 49% Exhaust Configuration Low Exhaust Exhaust Temperature (°C) RH 25 50% Change Rack Rack Station Orientation Density ON On wall Single Number of Mice in Room 1050 Total mass of Mice in Room 21000 gr Room Pressurisation neg lOOcfm Room ACH 15 Cage Condition Top On 7agn5mIpie^^onr Analysis Results Volume II - Section IV - Whole Room Simulation Database Page !I - 257 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day COj NHj 1 785000 2 785000 795 3 7^5000 1225 4 785000 TfW 5 TSW 2483 6 785000 7 785000 4712 8 785000 ~5JW 9 785000 8442 10 785000 11384 Cage occupied zone average relative humidity (%) distribution Volume II -258 Design Handbook on Animal Research Facilities Using Static Microisolators Casename Case 101 Description Supply Supply Discharge Supply Discharge Exhaust Exhaust Exhaust Configuration Temperature (°C) RH Configuration Temperature (°C) RH Low Ind 22 49% Low 25 50% Change Rack Rack Number of Total mass of Room Station Orientation Density Mice in Room Mice in Room Pressurisation ON alt design Perp Double 1050 21000 gr neg lOOcfm Room ACH 5 Cage Condition Top On Cage O ccuple^^r Analysis Results Temperature — usr~ — m — T (ppm) Mean 26,24 79.22 284 39.78% S.D. 1.52 2.74 00 Max. 28.22 82.79 793 Room Breathing Zone NH3 (ppm) 15iy 1 2 3 3 5 5 7 g 5 m Mean 0.15 (120 on on 0:00 1.25 1.71 230 2.06 TOO Max. on 0.80 1.24 1.78 2.51 00 TIE 6.42 11.15 Volume II - Section IV - Whole Room Simulation Database Page I! - 259 Histogram Distributions Cage occupied zone average temperature (°C) distribution Cage occupied zone average contamination (kg/kg) distribution Contamination conversion factors (kg/kg ^ ppm) Day CO, NHj 1 7850O0 408 2 7«500() 795 3 785000 1225 4 7850O0 1766 5 785000 TtST 6 785000 3443 7 785000 4712 8 785000 6356 785000 8447 10 785000 11384 Cage occupied zone average relative humidity (%) distribution • LIBRARY NIH Amazing Rest Amazing Research. Amazing Heip. http;/ /nihlibrary.nih.gov 1 0 Center Drive Bethesda, MD 20892-1150 301-496-1080 U. S. DEPARTMENT OF HEALTH AND HUMAN SERVICES NATIONAL INSTITUTES OF HEALTH BETHESDA, MARYLAND